Desmogleins 1, 3, and E-cadherin immunohistochemical expression within mucocutaneous pemphigus vulgaris

Alshami, Muhanad L.; Aswad, Fawaz; Abdullah, Bashar H.

doi:10.11604/pamj.2022.42.186.35429

Cited by 4 publications

(4 citation statements)

References 33 publications

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“…The data displayed a right-skewed distribution, with the mean age around 45.36±10.60 years. The control group displayed a trend of decreasing ages, consistent with findings from previous research conducted in Iraq [ 1 ] and China [ 18 ], where the average age fell within the range of 41 to 44 years. These results contrast other findings [ 19 ], which suggested an average age range of 50 to 55 years.…”

Section: Discussionsupporting

confidence: 88%

“…Autoantibodies targeting desmoglein 3 and desmoglein 1 (Dsg3 and Dsg1) in the blood of PV patients lead to acantholysis, preventing keratinocytes from adhering to each other. These autoantibodies play a crucial role in the pathophysiology of PV, often leading to the rapid transformation of blisters into ulcers or bleeding erosions within 24 hours of eruption [ 1 ]. Globally, PV has an incidence ranging from 0.07 to 1.6 per 100,000 individuals and is associated with a mortality rate over 3.5 times higher than the general population.…”

Section: Introductionmentioning

confidence: 99%

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Cut-off values for IL-21 and IL-23 as biochemical markers for pemphigus vulgaris

Al-Hasnawi,

AL-Drobie

2023

JMedLife

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Pemphigus vulgaris (PV) is a potentially fatal mucocutaneous autoimmune disease characterized by severe skin lesions. Interleukin-21 (IL-21) and IL-23 have been linked to several autoimmune inflammatory diseases that may have a critical role in PV immunopathogenesis, including T-helper 17 (Th17) development. This study aimed to compare the serum levels of IL-21 and IL-23 in patients with PV and healthy controls. This case-control study included 90 participants (45 patients and 45 controls). Serum IL-21 and IL-23 were measured using the Sandwich-ELISA method provided by Sunlong Biotech, China. The findings revealed statistically significant results for IL-21 O.D. and Conc. (p=0.012*) and highly significant results for IL-23 O.D. and Conc. (p=0.000**). Furthermore, cut-off values were established for IL-21 (O.D.=0.071 pg/mL, Conc.=6.468 pg/mL) and IL-23 (O.D.=0.141 pg/mL, Conc.=6.745 pg/mL). These results indicate a potential association between PV and IL-21, IL-23, and the identified cut-off values. The particular roles of cytokines and how they can be utilized to treat PV require more investigation. To our knowledge, this was the first study to detect a cut-off point for IL-21 and IL-23 that may be used as novel and cost-effective biochemical markers for disease diagnosis.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Cut-off values for IL-21 and IL-23 as biochemical markers for pemphigus vulgaris

Al-Hasnawi,

AL-Drobie

2023

JMedLife

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“…The ST18 gene has emerged as a potential contributor, potentially increasing the risk of PV in certain populations, and could represent a new potential molecular target for the treatment of disease. PV is a relatively rare disease, affecting approximately 0.07 to 1.6 per 100,000 people annually, with uneven geographic distribution [ 1 ]. It has been associated with a 2.3-3.3-fold increased fatality rate compared to the general population, primarily due to complications such as pneumonia or sepsis [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Assessing ST18 gene polymorphisms (rs17315309, rs2304365) in Iraqi patients with Pemphigus vulgaris

Ali Al-Hasnawi,

AL-Drobie

2024

JMedLife

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“…The CDH1 gene, located on chromosome 16q22.1, encodes the 120-kDa E-cadherin protein, a member of the cadherin family responsible for calcium-dependent cell adhesion and tissue organization [9] . E-cadherin interacts with catenins to achieve this function and has five extracellular domains with calcium-binding sites critical for adhesion stabilization [10,11] .…”

Section: Introductionmentioning

confidence: 99%

Estimation of gene expression level of CDH1 as a predisposing factor for metastasis in localized and locally advanced and metastatic breast cancer Iraqi patients

Abdallah,

Aziz,

Alsammarraie

2023

Trends Immunother.

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Breast cancer (BC) is a prevalent malignancy among women, ranking as the second most commonly diagnosed cancer globally. Notably, a substantial proportion of breast cancer-related fatalities, up to 90 percent, are attributed to the development of distant organ metastases. While Cadherin1 (CDH1) has conventionally been considered a tumor-suppressor gene in cancer research, recent investigations have unequivocally revealed that both CDH1 and its encoded E-cadherin exhibit oncogenic characteristics. The primary focus of this case-control study is to ascertain the involvement of the CDH1 gene in a specific subset of Iraqi female patients. A total of ninety patients sought diagnosis and treatment at the Oncology Teaching Hospital in Medical City and the Oncology Unit at Al-Yarmouk Teaching Hospital in Baghdad. In addition, we included 30 apparently healthy individuals as blood control subjects and another 30 women with benign breast tumors as tissue control subjects for the study. In the initial phase of the study, we conducted a serological analysis using enzyme-linked immunosorbent assay (ELISA) to detect the concentration of E-cadherin in serum samples from two groups of BC patients. The group with locally advanced and metastatic BC exhibited a significantly higher E-cadherin concentration (963.4 ± 89.8 pg/mL) compared to the group with localized BC. In the second part of the research, qRT-PCR was performed to analyze the expression of the CDH1 gene across all sample types. CDH1 was shown to have the greatest fold expression (2.550 ± 0.164) in cases with locally progressed and metastatic BC. Compared to the seemingly healthy control group, the fold expression for localized BC was 1.456 ± 0.055, and for malignant tissue it was 1.886 ± 0.08621. In conclusion, this study provides compelling evidence supporting CDH1 as an oncogene in BC. The significance of CDH1 in BC tumorigenesis underscores its potential for the development of novel detection biomarkers and targeted therapeutic approaches for BC treatment. Notably, CDH1 exhibited elevated expression levels in BC tissues and demonstrated an association with an unfavorable distant metastasis-free survival outcome.

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Desmogleins 1, 3, and E-cadherin immunohistochemical expression within mucocutaneous pemphigus vulgaris

Cited by 4 publications

References 33 publications

Cut-off values for IL-21 and IL-23 as biochemical markers for pemphigus vulgaris

Cut-off values for IL-21 and IL-23 as biochemical markers for pemphigus vulgaris

Assessing ST18 gene polymorphisms (rs17315309, rs2304365) in Iraqi patients with Pemphigus vulgaris

Estimation of gene expression level of CDH1 as a predisposing factor for metastasis in localized and locally advanced and metastatic breast cancer Iraqi patients

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