Desmosome development in normal and reassociating cells in the early chick blastoderm

Overton, Jane

doi:10.1016/0012-1606(62)90056-8

Cited by 144 publications

(60 citation statements)

References 11 publications

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“…A possible morphological vehicle, therefore, exists for the general selective, weakly adhesive properties which cells are known to possess (Moscona, 1960;Steinberg, 1964). In view of the added accumulation of such extracellular material as pillars, partitions, strands, etc., as integral components of fixed desmosomes and hemidesmosomes, a structural basis can be envisioned for Steinberg's concept that these attachments are strong ones, supplementary to the general adhesive tendencies of a given cell (see also Overton, 1962). The participation of intracellular filaments in the formation of such strong attachment zones is probably not directly related to adhesion per se, but rather to structural support of the cell skeleton at the attachment sites.…”

Section: Concluding Commentsmentioning

confidence: 99%

“…Barr, 1964), the "septate desmosomes" in epithelia of invertebrates (Wood, 1959;Locke, 1965), and synaptic contacts between neuronal, sensory, and muscular components. Such junctions have been considered as sites of heightened cell-to-cell permeability or impulse transmission, but the adhesive tenacity of many such sites, as deduced from their persistent attachment during microdissection (Chambers and de R6nyi, 1925 [see discussion by Odland, 1958 Overton (1962) described the re-establishment of desmosomes as subsequent to the reaggregation of dissociated chick blastoderm cells and noted a correlation in increasing cell-to-cell adhesivity with that re-establishment. Whether or not the stronger attachment sites play any role in selective, aggregative adhesion, it appears probable that the quality, number, and distribution of firm attachment mechanisms at the boundaries of a differentiated cell are related to the proper maintenance of position, morphology, and function of that cell.…”

Section: Introductionmentioning

confidence: 99%

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Fine Structure of Desmosomes, Hemidesmosomes, and an Adepidermal Globular Layer in Developing Newt Epidermis

Kelly

1966

The Journal of Cell Biology

420

109

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A B S T R A C TThe skin of late embryonic, larval, and young postmetamorphic newts, Taricha torosa, has been examined with particular reference to areas of cellular attachment. Stereo electron microscopic techniques and special staining methods for extracellular materials were utilized in addition to conventional avenues of ultrastructural study to investigate the fine architecture of desmosomes, hemidesmosomes, their associated filament systems, and extracellular materials. No evidence has been found that continuity of tonofilaments between adjacent cells exists at desmosomes. Rather, most of the tonofilaments which approach desmosomes (and perhaps also hemidesmosomes) course toward the "attachment plaque" and then loop, either outside the plaque or within it, and return into the main filament tracts of the cell. These facts suggest that the filamentous framework provides intracellular tensile support while adhesion is a product of extracellular materials which accumulate at attachment sites. Evidence is presented that the extracellular material is arranged as pillars or partitions which are continuous with or layered upon the outer unit cell membrane leaflets and adjoined in a discontinuous dense midline of the desmosome. A similar analysis has been made of extracellular materials associated with hemidesmosomes along the basal surface of epidermal cells. An adepidermal globular zone, separating the basal cell boundary from the underlying basal lamina and collagenous lamellae during larval stages, has been interpreted from enzyme and solvent extraction study as a lipid-mucopolysaccharide ccmplex, the function of which remains obscure. These observations are discussed in relation to prevailing theories of cellular adhesion and epidermal differentiation. They appear consistent with the concept that a wide range of adhesive specializations exists in nature, and that the more highly organized of these, such as large desmosomes and hemidesmosomes, serve as strong, highly supported attachment sites, supplemental in function to a more generalized aggregating mechanism.

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Section: Concluding Commentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fine Structure of Desmosomes, Hemidesmosomes, and an Adepidermal Globular Layer in Developing Newt Epidermis

Kelly

1966

The Journal of Cell Biology

420

109

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“…Early morphological studies demonstrated that desmosomes are assembled rapidly on the plasma membrane of adjacent epithelial cells upon induction of ceil-cell contact (30). Significantly, assembly appeared to occur from preex-isting pools of proteins in the cytoplasm and on the plasma membrane.…”

mentioning

confidence: 99%

Regulation of desmosome assembly in MDCK epithelial cells: coordination of membrane core and cytoplasmic plaque domain assembly at the plasma membrane.

Pasdar¹,

Krzeminski²,

Nelson³

1991

The Journal of Cell Biology

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Abstract. Desmosomes are major components of the intercellular junctional complex in epithelia. They consist of at least eight different cytoplasmic and integral membrane proteins that are organized into two bioehemically and structurally distinct domains: the cytoplasmic plaque and membrane core. We showed previously that in MDCK epithelial cells major components of the cytoplasmic plaque (desmoplakin I and 1I; DPI/II) and membrane core domains (desmoglein I; DGI) initially enter a pool of proteins that is soluble in buffers containing Triton X-100, and then titrate into an insoluble pool before their arrival at the plasma membrane (Pasdar, M., and W. J. Nelson. 1988. J. Cell Biol. 106:677-685; Pasdar, M., and W. J. Nelson. 1989. J. Cell Biol. 109:163-177). We have now examined whether either the soluble or insoluble pool of these proteins represents an intracellular site for assembly and interactions between the domains before their assembly into desmosomes at the plasma membrane. Interactions between the Triton X-100-soluble pools of DPI/II and DGI were analyzed by sedimentation of extracted proteins in sucrose gradients. Results show distinct differences in the sedimentation profiles of these proteins, suggesting that they are not associated in the Triton X-100-soluble pool of proteins; this was also supported by the observation that DGI and DPI/II could not be coimmunoprecipitated in a complex with each other from sucrose gradient fractions. Immunofluorescence analysis of the insoluble pools of DPI/II and DGI, in cells in which desmosome assembly had been synchronized, showed distinct differences in the spatial distributions of these proteins. Furthermore, DPI/II and DGI were found to be associated with different elements of cytoskeleton; DPI/II were located along cytokeratin intermediate filaments, whereas DGI appeared to be associated with microtubules. The regulatory role of cytoskeletal elements in the intracellular organization and assembly of the cytoplasmic plaque and membrane core domains, and their integration into desmosomes on the plasma membrane is discussed.

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“…Other contact specializations, the zonula adhaerens and macula adhaerens (desmosorne), are not present in the young tissues, but they do appear subsequently in the embryonic tissues (5,11).…”

Section: Resultsmentioning

confidence: 99%

“…Once embryonic cells have begun a course of somatic differentiation, they are characterized by a remarkable degree of adhesiveness which can be disrupted by trypsin in calcium-poor solutions (1-3) and which seems attributable in part to desmosomelike specializations, the zonula adhaerens and macula adhaerens (4)(5). Another cell contact specialization, one which has received little attention from embryologists, is the so-called tight junction which has been reported to take the form of a zonula, macula, or fascia occludens in mature tissues (4, 10).…”

mentioning

confidence: 99%

Tight Junctions Between Cells in the Early Chick Embryo as Visualized With the Electron Microscope

Trelstad

Revel

Hay

1966

The Journal of Cell Biology

107

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Desmosome development in normal and reassociating cells in the early chick blastoderm

Cited by 144 publications

References 11 publications

Fine Structure of Desmosomes, Hemidesmosomes, and an Adepidermal Globular Layer in Developing Newt Epidermis

Fine Structure of Desmosomes, Hemidesmosomes, and an Adepidermal Globular Layer in Developing Newt Epidermis

Regulation of desmosome assembly in MDCK epithelial cells: coordination of membrane core and cytoplasmic plaque domain assembly at the plasma membrane.

Tight Junctions Between Cells in the Early Chick Embryo as Visualized With the Electron Microscope

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