Desolvation Conditions for Production of Sulfasalazine Based Albumin Nanoparticles: Physical Properties

Abstract

Background: Albumin-based nanoparticles have been used as drug carriers to improve therapeutic effect. This study aimed to produce sulfasalazine (SSZ)-loaded bovine serum albumin (BSA) nanoparticles by desolvation technique and characterize their physical properties. Drug loading effect was also investigated.Methods: SSZ albumin nanoparticles were batch produced by desolvation technique and lyophilized. Physical characteristics of nanoparticles including size distribution, zeta potential and entrapment efficiency were studied.Results: Nanoparticles were spherical and typically 200-400 nm with low polydispersity (PDI). The zeta potentials of nanoparticles ranged from −21 to −41 mV, which were attributed to BSA and SSZ. The highest actual drug loading was close to 0.5%w/w. The addition of 2% sucrose during lyophilization was essential to avoid cake collapse and the particle sizes increased slightly where their PDI values were low. Although the presence of sucrose led to a slight reduction in zeta potential, it had limited effect on zeta potential of the nanoparticles. The interactions between SSZ and formulation excipients were also confirmed with FTIR and DSC analyses.Conclusion: Desolvation technique produced stable sulfasalazine-loaded nanoparticles that can be administered parenterally.

Crafting Docetaxel-Loaded Albumin Nanoparticles Through a Novel Thermal-Driven Self-Assembly/Microfluidic Combination Technology: Formulation, Process Optimization, Stability, and Bioavailability

“…DSC is a technique that monitors the physical properties of a substance by employing a controlled temperature program. 68 , 69 The DSC spectra of DTX; BSA; physical mixture of DTX, BSA, and sodium gluconate; and DTX-BSA-NPs are illustrated in Figure 8 . The endothermic peak of DTX around 170°C was found, which is consistent with the reported melting point range of DTX in literature.…”

Factors Affecting the Synthesis of Bovine Serum Albumin Nanoparticles Using the Desolvation Method

“…66,81 Furthermore, the increased viscosity reduces the number of collisions and mass transfer due to diffusion. 64 Based on the literature review in Table 2, three studies conducted by Olaitan and Chaw, 67 Wilson et al 30 and Aljabali et al 59 discussed the effect of drug concentration on characteristics of BSA nanoparticles formed. Olaitan and Chaw obtained the best characterization results at a Sulfasalazine concentration of 1%, Aljabali et al at a Piceatannol concentration of 0.125%, and Wilson et al at a 5-fluorouracil concentration of 0.25%.…”