Desorption/Chemical Ionization Mass Spectrometry of Naturally Occurring Glycosides

Abstract

SummaryDesorptionlchemical ionization mass spectrometry (D/CI.-MS.) is a recently developed technique especially indicated for highly polar and non volatile compounds. Various naturally occurring glycosides such as saponins, iridoid and secoiridoid glycosides, cardenolides and flavone-0-glycosides have been investigated by this method. All the measurements were carried out on underivatized compounds. In addition to the structural informations generally furnished by field-desorption mass spectrometry (molecular ion and sugar sequence), the molecular ion and pertinent fragments of the aglycone could also be obtained.

Structural analysis of saponins from medicinal herbs using electrospray ionization tandem mass spectrometry

“…In early studies, derivatization was required for saponin analysis using electron impact (EI) MS [7][8][9]. The development of desorption chemical ionization mass spectrometry (DCI-MS) allowed analysis of saponins without derivatization, but only for saponins with ether glycosidic linkages [10,11]. Later, field desorption (FD) and fast atom bombardment (FAB) were also employed to analyze native saponins [12][13][14][15][16][17][18], providing information about molecular mass and sugar sequence by cleavage of glycosidic bonds.…”

Desorption/Chemical Ionization Mass Spectrometry of Bitter Glycosides fromGentiana1

“…These inconveniences do not arise from desorption/chemical ionization mass spectroscopy (D/CI-MS) (6). We have demonstrated that this technique can be successfully used with underivatized naturally occurring glycosides such as saponins and flavonoid-Oglycosides (7,8). The present work reports on the application of D/CI-MS to some of the most important bitter principles which have been isolated in the course of our systematic investigation of Gentiana species.…”

Mass spectrometry of iridoids

“…Some underivatized iridoids and secoiridoids (77) have been investigated by this technique with ammonia as a reagent gas. The quasimolecular ion (M + NH,)+ forms the base peak.…”