Desorption electrospray ionization-mass spectrometry imaging of carnitine and imidazole dipeptides in pork chop tissues

Imidazole dipeptides (IDPs) are found in the skeletal muscles and brains of various animals, and they exhibit unique functions like antioxidant and antiaging properties. Despite their importance, the metabolic mechanisms and physiological roles of IDPs remain unclear. Herein, we propose a method for the simultaneous analysis of IDPs, their constituent amino acids, and taurine, which are difficult to separate using conventional labeling reagents or columns, using liquid chromatography−single quadrupole mass spectrometry with PBr column and our highly sensitive labeling reagent, 1-fluoro-2,4-dinitrophenyl-5-L-valine-N,Ndimethylethylenediamineamide (L-FDVDA). This method successfully separated histidine and carnosine enantiomers as well as L-2oxocarnosine with high antioxidant activity under the same conditions. Our labeling reagent was more stable than the other reagents and did not degrade and desorb from the analytes for at least 1 week at 4 °C. Furthermore, our method allows for the accurate analysis of IDPs, amino acids, and taurine in meats from various animal species, tissues, and breeds.

show abstract

Revolutionizing food science with mass spectrometry imaging: A comprehensive review of applications and challenges

Shen,

Wang,

Wang

et al. 2024

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

Food science encounters increasing complexity and challenges, necessitating more efficient, accurate, and sensitive analytical techniques. Mass spectrometry imaging (MSI) emerges as a revolutionary tool, offering more molecular‐level insights. This review delves into MSI's applications and challenges in food science. It introduces MSI principles and instruments such as matrix‐assisted laser desorption/ionization, desorption electrospray ionization, secondary ion mass spectrometry, and laser ablation inductively coupled plasma mass spectrometry, highlighting their application in chemical composition analysis, variety identification, authenticity assessment, endogenous substance, exogenous contaminant and residue analysis, quality control, and process monitoring in food processing and food storage. Despite its potential, MSI faces hurdles such as the complexity and cost of instrumentation, complexity in sample preparation, limited analytical capabilities, and lack of standardization of MSI for food samples. While MSI has a wide range of applications in food analysis and can provide more comprehensive and accurate analytical results, challenges persist, demanding further research and solutions. The future development directions include miniaturization of imaging devices, high‐resolution and high‐speed MSI, multiomics and multimodal data fusion, as well as the application of data analysis and artificial intelligence. These findings and conclusions provide valuable references and insights for the field of food science and offer theoretical and methodological support for further research and practice in food science.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Desorption electrospray ionization-mass spectrometry imaging of carnitine and imidazole dipeptides in pork chop tissues

Cited by 3 publications

References 29 publications

Biological properties and methods for determination of carnosine

Biological properties and methods for determination of carnosine

Simultaneous Analysis of Imidazole Dipeptides, Constituent Amino Acids, and Taurine in Meats Using the Highly Sensitive Labeling Reagent l-FDVDA and PBr Column

Revolutionizing food science with mass spectrometry imaging: A comprehensive review of applications and challenges

Contact Info

Product

Resources

About