Destabilization of ROS metabolism in tomato roots as a phytotoxic effect of meta -tyrosine

Andrzejczak, Olga; Krasuska, Urszula; Olechowicz, Joanna; Staszek, Paweł; Ciąćka, Katarzyna; Bogatek, Renata; Hebelstrup, Kim H.; Gniazdowska, Agnieszka

doi:10.1016/j.plaphy.2017.12.024

Cited by 13 publications

(17 citation statements)

References 33 publications

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“…Rapid increase in H 2 O 2 level after R5 treatment (about 50% higher compared to control) represents the typical reaction to allelochemicals since the similar effect was annotated after application of numerous other allelochemicals (Soltys et al 2011(Soltys et al , 2014Guan et al 2014;Krasuska et al 2016;Andrzejczak et al 2018). Different increments of H 2 O 2 roots and shoots content could be explained by differential activation of enzymatic antioxidant defense system, observed in wheat shoot and root in the previous study (Dmitrović et al 2015a).…”

Section: Discussionsupporting

confidence: 50%

Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate

et al. 2018

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Chenopodium murale L. is an invasive weed species significantly interfering with wheat crop. However, the complete nature of its allelopathic influence on crops is not yet fully understood. In the present study, the focus is made on establishing the relation between plant morphophysiological changes and oxidative stress, induced by allelopathic extract. Phytotoxic medium of C. murale hairy root clone R5 reduced the germination rate (24% less than control value) of wheat cv. Nataša seeds, as well as seedling growth, diminishing shoot and root length significantly, decreased total chlorophyll content, and induced abnormal root gravitropism. The R5 treatment caused cellular structural abnormalities, reflecting on the root and leaf cell shape and organization. These abnormalities mostly included the increased number of mitochondria and reorganization of the vacuolar compartment, changes in nucleus shape, and chloroplast organization and distribution. The most significant structural changes were observed in cell wall in the form of amoeboid protrusions and folds leading to its irregular shape. These structural alterations were accompanied by an oxidative stress in tissues of treated wheat seedlings, reflected as increased level of HO and other ROS molecules, an increase of radical scavenging capacity and total phenolic content. Accordingly, the retardation of wheat seedling growth by C. murale allelochemicals may represent a consequence of complex activity involving both cell structure alteration and physiological processes.

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Section: Discussionsupporting

confidence: 50%

Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate

et al. 2018

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“…Exposition of tomato to meta -tyrosine (NPAA released into the environment as root exudates of fescues) led to an increased GSNOR activity and higher abundance of the protein (Krasuska et al, 2017). Although the morphological consequences of CAN supplementation in tomato seedlings were similar to that observed after meta -tyrosine (Andrzejczak et al, 2018), its impact on GSNOR was opposite. We noticed slightly higher content of the protein, only small inhibition of the enzyme after short-term treatment with CAN and constant activity as the experiment was prolonged.…”

Section: Discussionmentioning

confidence: 61%

“…It cannot be excluded that relatively high total antioxidant capacity in CAN-exposed roots may be due to a stimulation of biosynthesis of other nonenzymatic antioxidants, particularly phenolic compounds. Phenols accumulation was observed in tomato roots treated with meta -tyrosine, and we proposed that it could be regarded as a protective mechanism against stress induced by this NPAA (Andrzejczak et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Canavanine-Induced Decrease in Nitric Oxide Synthesis Alters Activity of Antioxidant System but Does Not Impact S-Nitrosoglutathione Catabolism in Tomato Roots

et al. 2019

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Canavanine (CAN) is a nonproteinogenic amino acid synthesized in legumes. In mammalians, as arginine analogue, it is an inhibitor of nitric oxide synthase (NOS) activity. The aim of this study was to investigate the impact of CAN-induced nitric oxide level limitation on the antioxidant system and S-nitrosoglutathione (GSNO) metabolism in roots of tomato seedlings. Treatment with CAN (10 or 50 µM) for 24–72 h led to restriction in root growth. Arginine-dependent NOS-like activity was almost completely inhibited, demonstrating direct effect of CAN action. CAN increased total antioxidant capacity and the level of sulphydryl groups. Catalase (CAT) and superoxide dismutase (SOD) activity decreased in CAN exposed roots. CAN supplementation resulted in the decrease of transcript levels of genes coding CAT (with the exception of CAT1). Genes coding SOD (except MnSOD and CuSOD) were upregulated by CAN short treatment; prolonged exposition to 50-µM CAN resulted in downregulation of FeSOD, CuSOD, and SODP-2. Activity of glutathione reductase dropped down after short-term (10-µM CAN) supplementation, while glutathione peroxidase activity was not affected. Transcript levels of glutathione reductase genes declined in response to CAN. Genes coding glutathione peroxidase were upregulated by 50-µM CAN, while 10-µM CAN downregulated GSHPx1. Inhibition of NOS-like activity by CAN resulted in lower GSNO accumulation in root tips. Activity of GSNO reductase was decreased by short-term supplementation with CAN. In contrast, GSNO reductase protein abundance was higher, while transcript levels were slightly altered in roots exposed to CAN. This is the first report on identification of differentially nitrated proteins in response to supplementation with nonproteinogenic amino acid. Among nitrated proteins differentially modified by CAN, seed storage proteins (after short-term CAN treatment) and components of the cellular redox system (after prolonged CAN supplementation) were identified. The findings demonstrate that due to inhibition of NOS-like activity, CAN leads to modification in antioxidant system. Limitation in GSNO level is due to lower nitric oxide formation, while GSNO catabolism is less affected. We demonstrated that monodehydroascorbate reductase, activity of which is inhibited in roots of CAN-treated plants, is the protein preferentially modified by tyrosine nitration.

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“…commutata and F. rubra spp. rubra ) plants [ 74 ]. The protein nitration level measured as a content of 3-nitro-tyrosine increased in roots of tomato plants after their treatment with meta -tyrosine ( Table S1 ) [ 73 ] and fluctuated depending on the duration of the treatment, modifying the intracellular formation of RNS (mainly ONOO − ) after canavanine application [ 75 , 110 ].…”

Section: Induction Of Oxidative Stress As a Secondary Mode Of Action Of Phytotoxinsmentioning

confidence: 99%

ROS Metabolism Perturbation as an Element of Mode of Action of Allelochemicals

et al. 2021

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The allelopathic interaction between plants is one of the elements that influences plant communities. It has been commonly studied by applying tissue extracts onto the acceptors or by treating them with isolated allelotoxins. Despite descriptive observations useful for agricultural practice, data describing the molecular mode of action of allelotoxins cannot be found. Due to the development of -omic techniques, we have an opportunity to investigate specific reactive oxygen species (ROS)-dependent changes in proteome or transcriptome that are induced by allelochemicals. The aim of our review is to summarize data on the ROS-induced modification in acceptor plants in response to allelopathic plants or isolated allelochemicals. We present the idea of how ROS are involved in the hormesis and plant autotoxicity phenomena. As an example of an -omic approach in studies of the mode of action of allelopatic compounds, we describe the influence of meta-tyrosine, an allelochemical exudated from roots of fescues, on nitration—one of nitro-oxidative posttranslational protein modification in the roots of tomato plants. We conclude that ROS overproduction and an induction of oxidative stress are general plants’ responses to various allelochemicals, thus modification in ROS metabolisms is regarded as an indirect mode of action of allelochemicals.

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Destabilization of ROS metabolism in tomato roots as a phytotoxic effect of meta -tyrosine

Cited by 13 publications

References 33 publications

Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate

Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate

Canavanine-Induced Decrease in Nitric Oxide Synthesis Alters Activity of Antioxidant System but Does Not Impact S-Nitrosoglutathione Catabolism in Tomato Roots

ROS Metabolism Perturbation as an Element of Mode of Action of Allelochemicals

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