Destructing biofilms by cationic dextran through phase transition

Li, Yurong; Wang, Shaocong; Xing, Zhen; Niu, Yiming; Liao, Zhencheng; Lü, Yang; Qiu, Junni; Zhang, Junfeng; Wang, Chunming; Dong, Lei

doi:10.1016/j.carbpol.2021.118778

Cited by 11 publications

(5 citation statements)

References 65 publications

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“…Zeta potential plays a crucial role in determining the physiological stability of cationic polymers. For instance, cationic dextrans such as CDI-dextran (C-Dex) and EDTA-dextran (EDTA-Dex), which possess a zeta potential of approximately 30-35 mV, have demonstrated the ability to disrupt biofilms and effectively control wound infections [27]. However, the zeta potential of dextran PEI imidazole (DPI) was found to be lower, ranging from 16-22 mV, which is below the values observed in this study [28].…”

Section: Preparation Of Positively Charged Is-dextran With Peicontrasting

confidence: 59%

Preparation and Characterization of a Novel Morphosis of Dextran and Its Derivatization with Polyethyleneimine

Jiang,

Sun,

et al. 2023

Molecules

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Dextran, a variant of α-glucan with a significant proportion of α-(1,6) bonds, exhibits remarkable solubility in water. Nonetheless, the precipitation of dextran has been observed in injection vials during storage. The present study aimed to establish a technique for generating insoluble dextran and analyze its structural properties. Additionally, the potential for positively ionizing IS-dextran with polyethyleneimine was explored, with the ultimate objective of utilizing IS-dextran-PEI as a promising support for enzyme immobilization. As a result, IS-dextran was obtained by the process of slow evaporation with an average molecular weight of 6555 Da and a yield exceeding 60%. The calculated crystallinity of IS-dextran, which reaches 93.62%, is indicative of its irregular and dense structure, thereby accounting for its water insolubility. Furthermore, positive charge modification of IS-dextran, coupled with the incorporation of epichlorohydrin, resulted in all zeta potentials of IS-dextran-PEIs exceeding 30 mV, making it a promising supporting factor for enzyme immobilization.

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Section: Preparation Of Positively Charged Is-dextran With Peicontrasting

confidence: 59%

Preparation and Characterization of a Novel Morphosis of Dextran and Its Derivatization with Polyethyleneimine

Jiang,

Sun,

et al. 2023

Molecules

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“…Cationic polymers were synthesized according to a previously reported method [ 17 , 36 ]. Briefly, for cDex, 0.3 g of dextran dissolved in DMSO (30 ml) was incubated with 0.9 g of CDI for activation.…”

Section: Methodsmentioning

confidence: 99%

Intracellular mRNA phase separation induced by cationic polymers for tumor immunotherapy

Xing

Xue²,

Ma³

et al. 2022

J Nanobiotechnol

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The formation of biomolecular condensates via liquid‒liquid phase separation (LLPS) is an advantageous strategy for cells to organize their subcellular compartments for diverse functions. Recent findings suggest that RNA or RNA-related LLPS techniques have potential for the development of new cellular regulation strategies. However, manipulating RNA LLPS in living cells has great challenges. Herein, we report that cationic polymers (CPs) have strong RNA LLPS-inducing activity. By introducing CPs into living cells or RNA solutions, significant RNA LLPS was verified through confocal imaging, turbidity assays, and fluorescence recovery after photobleaching (FRAP) tests. Among them, turbidity kinetics determinations indicated that the hydrophilic positively charged amino groups on the CPs play essential roles in RNA phase separation. Moreover, the LLPS induced by the cationic polymers dramatically changed the gene expression patterns in the cells. Interestingly, we found that TGFβ1 mRNA was highly encapsulated in the RNA droplets, which lowered the immunosuppressive capability of the tumor cells and triggered marked antitumor reactions in a mouse breast cancer model. Thus, we present here the CP-based modulation of RNA LLPS as a novel transcriptional manipulation method with potential for cancer immunotherapy drug development. Graphical Abstract

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“…Recognizing dextran's affinity for the polysaccharides in the biofilm matrix, Li et al employed cationic dextran to disrupt the intrinsic electrostatic interaction in the biofilm matrix. The biofilm matrix, maintained by the positively charged Pel (positively charged polysaccharide) and eDNA, underwent a gel-to-sol phase transition when disrupted by the cationic dextran, leading to a collapse of the biofilm's structural integrity (Figure 3f) [165]. This particular form of cationic dextran showed enhanced antibacterial capability against P. aeruginosa biofilms, positioning it as a promising cationic substance to combat bacterial biofilm through inducing phase transitions.…”

Section: Dextran Antimicrobial Nanoparticlesmentioning

confidence: 99%

“…[160]); (f) a diagram illustrating the disruption of bacterial biofilm by cationic dextran through the phase transition (Ref. [165]); (g) schematic representation of the preparation of Lipid@PLL-PS NPs and Lipid@DOTAP-PS NPs using the electrostatically driven layer-by-layer method (Ref. [156]).…”

Section: Amphiphilic Cationic Copolymer Antimicrobial Nanoparticlesmentioning

confidence: 99%

Nanotechnology-Based Drug Delivery Systems to Control Bacterial-Biofilm-Associated Lung Infections

Guo,

Mao,

Ran

et al. 2023

Pharmaceutics

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Airway mucus dysfunction and impaired immunological defenses are hallmarks of several lung diseases, including asthma, cystic fibrosis, and chronic obstructive pulmonary diseases, and are mostly causative factors in bacterial-biofilm-associated respiratory tract infections. Bacteria residing within the biofilm architecture pose a complex challenge in clinical settings due to their increased tolerance to currently available antibiotics and host immune responses, resulting in chronic infections with high recalcitrance and high rates of morbidity and mortality. To address these unmet clinical needs, potential anti-biofilm therapeutic strategies are being developed to effectively control bacterial biofilm. This review focuses on recent advances in the development and application of nanoparticulate drug delivery systems for the treatment of biofilm-associated respiratory tract infections, especially addressing the respiratory barriers of concern for biofilm accessibility and the various types of nanoparticles used to combat biofilms. Understanding the obstacles facing pulmonary drug delivery to bacterial biofilms and nanoparticle-based approaches to combatting biofilm may encourage researchers to explore promising treatment modalities for bacterial-biofilm-associated chronic lung infections.

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Destructing biofilms by cationic dextran through phase transition

Cited by 11 publications

References 65 publications

Preparation and Characterization of a Novel Morphosis of Dextran and Its Derivatization with Polyethyleneimine

Preparation and Characterization of a Novel Morphosis of Dextran and Its Derivatization with Polyethyleneimine

Intracellular mRNA phase separation induced by cationic polymers for tumor immunotherapy

Nanotechnology-Based Drug Delivery Systems to Control Bacterial-Biofilm-Associated Lung Infections

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