Destruction of shells and release of a protein from microcapsules consisting of non-biodegradable polyelectrolytes

Дубровский, А. В.; Kochetkova, O. Yu.; Kim, Aleksandr L.; Musin, Egor V.; Seraya, Olga; Tikhonenko, Sergey A.

doi:10.1080/00914037.2018.1429436

Cited by 8 publications

(6 citation statements)

References 24 publications

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“…pKa of PSS is 1.22 [33]). When studying the capsules obtained in CaCO 3 -spherulites containing protein, the highest level reached polymer dissociation at 22 • C in a solution at pH 7, at 37 • C capsules become more resistant to degradation [27]. In the case of capsules spongy type of effect is observed, on the contrary, an increase of the medium temperature increases dissociation of the polymer dynamics, as in the case of the salts.…”

Section: Resultsmentioning

confidence: 95%

“…In our previous work [27], the dissociation of a different type of capsules-capsules formed on protein-containing CaCO 3 -spherulites-was studied. A decrease in the dissociation of polyelectrolytes with increasing temperature was also demonstrated.…”

Section: Resultsmentioning

confidence: 99%

“…An increase in the pH of the solution to 7.5 also causes peeling of PAH, however this effect decreases approximately by a factor of 2 with an increase in temperature to 37 • C due to the ordering and compaction of the envelope, which is shown by electron microscopic studies. In addition, it showed that the encapsulated protein practically does not leave the microcapsules, regardless of the presence of salts, their concentration and pH in the medium [27]. However, for some biologically active substances, such a procedure for inclusion in microcapsules cannot be used, due to aggressive pH values during the formation of spherulites, the possibility of elution of metal ions from the molecules of EDTA metal proteins, when dissolving them, etc.…”

Section: Introductionmentioning

confidence: 99%

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Destruction of Polyelectrolyte Microcapsules Formed on CaCO3 Microparticles and the Release of a Protein Included by the Adsorption Method

2020

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The degradation of polyelectrolyte microcapsules formed on protein-free CaCO3 particles consisting of polyallylamine (PAH) and polystyrene sulfonate (PSS) and the resulting yield of protein in the presence of various salts of different concentrations, as well as at two pH values, was studied by fluorescence spectroscopy; the protein was incorporated into prepared microcapsules by adsorption. It was found that a high concentration of sodium chloride (2 M) leads to considerable dissociation of PAH, which is apparently due to the loosening of polyelectrolytes under the action of ionic strength. At the same time, 0.2 M sodium chloride and ammonium sulfate of the same ionic strength (0.1 M) exert less influence on the amount of dissociated polymer. In the case of ammonium sulfate (0.1 M), the effect is due to the competitive binding of sulfate anions to the amino groups of the polyelectrolyte. However, unlike microcapsules formed on CaCO3 particles containing protein, the dissociation of polyelectrolyte from microcapsules formed on protein-free particles increased with increasing temperature. Apparently, a similar effect is associated with the absence of a distinct shell, which was observed on microcapsules formed on protein-containing CaCO3 particles. The high level of the presence of Fluorescein isothiocyanate (FITC)-labeled Bovine Serum Albumin (BSA) in the supernatant is explained by the large amount of electrostatically bound protein and the absence of a shell that prevents the release of the protein from the microcapsules. In 2 M NaCl, during the observation period, the amount of the released protein did not exceed 70% of the total protein content in the capsules, in control samples, this value does not exceed 8%, which indicates the predominantly electrostatic nature of protein retention in capsules formed on protein-free CaCO3 particles. The increase in protein yield and peeling of PAH with increasing pH is explained by the proximity of pH 7 to the point of charge exchange of the amino group of polyelectrolyte, as a result of the dissociation of the microcapsule.

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Destruction of Polyelectrolyte Microcapsules Formed on CaCO3 Microparticles and the Release of a Protein Included by the Adsorption Method

2020

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“…As seen above, many works have been devoted to the application of PMC in various fields. However, there are significantly fewer works studying the polyelectrolyte microcapsules themselves, in particular: the stability of the PMC shell [ 55 , 56 ], its surface charge [ 53 ], and ultrastructural organization [ 57 ], PMC buffer capacity [ 58 ], and the permeability of its shell [ 59 ]. Nevertheless, their physicochemical properties, internal structure, and mutual arrangement of polyelectrolytes in the PMC shell are poorly understood, although the study and understanding of these parameters are necessary for successful encapsulation of substances and predicting the effect of various conditions on the structure of the microcapsules themselves, as well as on the encapsulated substances.…”

Section: Introductionmentioning

confidence: 99%

Behaviour of FITC-Labeled Polyallylamine in Polyelectrolyte Microcapsules

et al. 2023

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There are many studies devoted to the application of polyelectrolyte microcapsules (PMC) in various fields; however, there are significantly fewer studies devoted to the study of the polyelectrolyte microcapsules themselves. The study examined the mutual arrangement of the polyelectrolytes in 13-layered PMC capsules composed of (PAH/PSS)6PAH. The research showed that different layers of the polyelectrolyte microcapsules dissociate equally, as in the case of 13-layered PMC capsules composed of (PAH/PSS)6PAH with a well-defined shell, and in the case of 7-layered PMC capsules composed of (PAH/PSS)3PAH, where the shell is absent. The study showed that polyallylamine layers labeled with FITC migrate to the periphery of the microcapsule regardless of the number of layers. This is due to an increase in osmotic pressure caused by the rapid flow of ions from the interior of the microcapsule into the surrounding solution. In addition, FITC-polyallylamine has a lower charge density and less interaction with polystyrene sulfonate in the structure of the microcapsule. Meanwhile, the hydrophilicity of FITC-polyallylamine does not change or decreases slightly. The results suggest that this effect promotes the migration of labeled polyallylamine to a more hydrophilic region of the microcapsule, towards its periphery.

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“…In addition, a number of works show changes in the PMC morphology, their cut thickness, and the migration of their polyelectrolyte layers, depending not only on the composition of the PMC shell, the presence of an encapsulated protein, or the presence of sodium chloride, but also on the ionic strength of the solution and the temperature of the medium [ 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ]. This may also indicate a possible dependence of the buffer capacity of polyelectrolyte microcapsules on these conditions.…”

Section: Introductionmentioning

confidence: 99%

A Study of the Buffer Capacity of Polyelectrolyte Microcapsules Depending on Their Ionic Environment and Incubation Temperature

Дубровский

Kim

Musin

et al. 2022

IJMS

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Polyelectrolyte microcapsules (PMCs) are used in the development of new forms of drugs, coatings and diagnostic systems. Their buffer capacity, depending on the conditions of the medium, has not been practically studied, although it can affect the structure of both the capsule itself and the encapsulated agents. In this connection, we studied the buffer capacity of polyelectrolyte microcapsules of the composition (polystyrene sulfonate/polyallylamine)3 ((PSS/PAH)3) depending on the concentration and the type of salt in solution, as well as the microcapsule incubation temperature. It was found that the buffer capacity of microcapsules in the presence of mono- and di-valent salts of the same ionic strength did not differ practically. Increasing the NaCl concentration to 1 M led to an increase of buffer capacity of PMCs at pH ≥ 5, and an increase in NaCl concentration above 1 M did not change buffer capacity. The study of the buffer capacity of pre-heated PMCs showed that buffer capacity decreased with increasing incubation temperature, which was possibly due to the compaction of the PMCs and an increase in the number of compensated PAH sites. The addition of 1 M sodium chloride to heated PMCs presumably reversed the process described above, since an increase in the ionic strength of the solution led to an increase of the buffer capacity of the PMCs. The effects described above confirm the hypothesis put forward that the buffer properties of microcapsules are determined by uncompensated PAH regions in their composition.

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Destruction of shells and release of a protein from microcapsules consisting of non-biodegradable polyelectrolytes

Cited by 8 publications

References 24 publications

Destruction of Polyelectrolyte Microcapsules Formed on CaCO3 Microparticles and the Release of a Protein Included by the Adsorption Method

Destruction of Polyelectrolyte Microcapsules Formed on CaCO3 Microparticles and the Release of a Protein Included by the Adsorption Method

Behaviour of FITC-Labeled Polyallylamine in Polyelectrolyte Microcapsules

A Study of the Buffer Capacity of Polyelectrolyte Microcapsules Depending on Their Ionic Environment and Incubation Temperature

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