Destructive and protective action of sodium dodecyl sulphate micelles on the native conformation of Bovine Serum Albumin: A study by extrinsic fluorescence probe 1-hydroxy-2-naphthaldehyde

Chemically Induced Unfolding of Bovine Serum Albumin by Urea and Sodium Dodecyl Sulfate: A Spectral Study with the Polarity‐Sensitive Charge‐Transfer Fluorescent Probe (E)‐3‐(4‐Methylaminophenyl)acrylic Acid Methyl Ester

“…The binding curve for BSA with SDS was constructed by plotting the fraction of the BSA molecules bound to SDS (a) against SDS concentration. [32] As was observed in many other cases before, this curve shows four distinct regions of binding between the SDS molecules and BSA. Initially, at very low SDS concentrations (0 to about 0.1 mm SDS), the surfactant molecules bind to some specific high-energy binding sites on BSA.…”

Spectroscopic probe analysis for exploring probe–protein interaction: A mapping of native, unfolding and refolding of protein bovine serum albumin by extrinsic fluorescence probe

“…Fluorescence spectroscopy serves as a feasible and powerful sensing tool for the investigation of local environment of the fluorophore yielding structural and dynamical information concerning the fluorophore environment [27][28][29]. Extrinsic fluorescence probe such as excited state charge, proton and electron transfer molecules can be used successfully for studying conformational changes of protein structure with variation of its solvent characteristics [30][31][32][33][34][35]. The molecule para-N,N-dimethylamino orthohydroxy benzaldehyde (PDOHBA) is one of the interesting organic molecule studied by our group which exhibits excited state coupled intramolecular charge transfer (CT) and proton transfer (PT) reaction [36].…”

Reply to comment on ‘Critical micellar concentration and protein–surfactant interaction (Comment to ‘Destructive and protective action of sodium dodecyl sulphate micelles on the native conformation of Bovine Serum Albumin: A study by extrinsic fluorescence probe 1-hydroxy-2-naphthaldehye’)’

“…4 and 5 of Ref. [1]. The authenticity of the work is itself established from the obtained values even though this was not mentioned keeping in mind readily available literature on this topic.…”

“…463 (2008) 183] aims at clarifying the points which Ragone seems to have drawn our attention to. We are thankful to Prof. Raffaele Ragone for his meticulous observation of our work and valuable comments concerning our measured critical micellar concentration (CMC) of surfactant sodium dodecyl sulphate (SDS) and the study of interaction of SDS with protein Bovine Serum Albumin (BSA) [1]. We want to mention here firstly that our work aimed at indicating the successful use of a proton transfer probe for the determination of CMC of SDS and monitor the folding and unfolding process of protein BSA using simple fluorimetric technique [2,3].…”