Desulfomarina profundi gen. nov., sp. nov., a novel mesophilic, hydrogen-oxidizing, sulphate-reducing chemolithoautotroph isolated from a deep-sea hydrothermal vent chimney

Abstract

A novel mesophilic, strictly anaerobic, chemolithoautotrophic sulphate-reducing bacterium, designated strain KT2T, was isolated from a deep-sea hydrothermal vent chimney at the Suiyo Seamount in the Izu-Bonin Arc. Strain KT2T grew at 25–40 °C (optimum 35 °C) and pH 5.5–7.0 (optimum 6.6) in the presence of 25–45 g l−1 NaCl (optimum 30 g l−1). Growth occurred with molecular hydrogen as the electron donor and sulphate, thiosulphate, and sulphite as the electron acceptors. The isolate utilized CO2 as the sole carbon source for chemolithoautotrophic growth on H2. Glycerol, succinate, fumarate, malate, glutamate, or casamino acids could serve as an alternative electron donor in the presence of CO2. Malate, citrate, glutamate, and casamino acids were used as fermentative substrates for weak growth. The G+C content of genomic DNA was 46.1 %. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain KT2T is a member of the family Desulfobulbaceae , showing a sequence similarity of 94.3 % with Desulforhopalus singaporensis . Phylogenomic analysis based on concatenated 156 single-copy marker genes confirmed the same topology as the 16S rRNA gene phylogeny. The ANI and AAI values between strain KT2T and related genera of the family Desulfobulbaceae were 65.6–68.6 % and 53.1–62.9 %. Based on the genomic, molecular, and physiological characteristics, strain KT2T represents a novel genus and species within the family Desulfobulbaceae , for which the name Desulfomarina profundi gen. nov., sp. nov. is proposed, with KT2T (=JCM 34118T = DSM 111364T) as the type strain.

Physiological and comparative proteomic characterization of Desulfolithobacter dissulfuricans gen. nov., sp. nov., a novel mesophilic, sulfur-disproportionating chemolithoautotroph from a deep-sea hydrothermal vent

“…Sample collection and subsampling were performed as described previously ( Hashimoto et al, 2021 ). Briefly, the chimney structure was obtained at the Suiyo Seamount, at a depth of 1,383 m ( Supplementary Figure 1 ).…”

“…The extraction of genomic DNA and determination and alignment of the sequence of the PCR product (1,490 bp) were performed as described previously ( Hashimoto et al, 2021 ). A phylogenetic tree was constructed as mentioned in ( Nagata et al, 2017 ).…”

“…Cells were observed using a light microscope (BX53, Olympus, Tokyo, Japan). Electron micrographs were captured using a transmission electron microscope (Tecnai G2 20; FEI, Hillsboro, OR) as described previously ( Hashimoto et al, 2021 ).…”

“…Cellular fatty acids, respiratory quinones, and polar lipids of strain GF1 T were extracted and analyzed as described previously ( Hashimoto et al, 2021 ). Cells of the late-exponential growth phase in MMJFE medium at 35°C were used.…”

“…The whole genome sequencing and the contamination and quality check of the genome were conducted as described in ( Hashimoto et al, 2021 ). The functional annotation was performed with DFAST ( Tanizawa et al, 2018 ) and RAST v2.0 ( Aziz et al, 2008 ).…”