Desulfovibrio tunisiensis sp. nov., a novel weakly halotolerant, sulfate-reducing bacterium isolated from exhaust water of a Tunisian oil refinery

Gam, Zouhaier Ben Ali; Oueslati, Ridha; Abdelkafi, Slim; Casalot, Laurence; Tholozan, Jean Luc; Labat, Marc

doi:10.1099/ijs.0.000943-0

Cited by 35 publications

(12 citation statements)

References 37 publications

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“…The description is the same as that for Desulfovibrio tunisiensis given by Ben Ali Gam et al . (2009) [136]. The type strain is RB22 T (=DSM 19275=JCM 15076).…”

Section: Discussionmentioning

confidence: 99%

Proposal to reclassify the proteobacterial classes Deltaproteobacteria and Oligoflexia, and the phylum Thermodesulfobacteria into four phyla reflecting major functional capabilities

Waite

Chuvochina

Pelikan

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

922

304

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The class Deltaproteobacteria comprises an ecologically and metabolically diverse group of bacteria best known for dissimilatory sulphate reduction and predatory behaviour. Although this lineage is the fourth described class of the phylum Proteobacteria , it rarely affiliates with other proteobacterial classes and is frequently not recovered as a monophyletic unit in phylogenetic analyses. Indeed, one branch of the class Deltaproteobacteria encompassing Bdellovibrio-like predators was recently reclassified into a separate proteobacterial class, the Oligoflexia . Here we systematically explore the phylogeny of taxa currently assigned to these classes using 120 conserved single-copy marker genes as well as rRNA genes. The overwhelming majority of markers reject the inclusion of the classes Deltaproteobacteria and Oligoflexia in the phylum Proteobacteria . Instead, the great majority of currently recognized members of the class Deltaproteobacteria are better classified into four novel phylum-level lineages. We propose the names Desulfobacterota phyl. nov. and Myxococcota phyl. nov. for two of these phyla, based on the oldest validly published names in each lineage, and retain the placeholder name SAR324 for the third phylum pending formal description of type material. Members of the class Oligoflexia represent a separate phylum for which we propose the name Bdellovibrionota phyl. nov. based on priority in the literature and general recognition of the genus Bdellovibrio. Desulfobacterota phyl. nov. includes the taxa previously classified in the phylum Thermodesulfobacteria , and these reclassifications imply that the ability of sulphate reduction was vertically inherited in the Thermodesulfobacteria rather than laterally acquired as previously inferred. Our analysis also indicates the independent acquisition of predatory behaviour in the phyla Myxococcota and Bdellovibrionota, which is consistent with their distinct modes of action. This work represents a stable reclassification of one of the most taxonomically challenging areas of the bacterial tree and provides a robust framework for future ecological and systematic studies.

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“…The description is the same as that for Desulfovibrio tunisiensis given by Ben Ali Gam et al . (2009) [136]. The type strain is RB22 T (=DSM 19275=JCM 15076).…”

Section: Discussionmentioning

confidence: 99%

Proposal to reclassify the proteobacterial classes Deltaproteobacteria and Oligoflexia, and the phylum Thermodesulfobacteria into four phyla reflecting major functional capabilities

Waite

Chuvochina

Pelikan

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

922

304

View full text Add to dashboard Cite

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“…The conditions under which sporulation was tested included growth in the absence of a carbon source or in the presence of yeast extract. Growth under anaerobic conditions was determined as described previously [18,19]. Gram reaction was determined using the BioMérieux Gram stain Kit according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Isolation of a novel amylase and lipase-producing Pseudomonas luteola strain: study of amylase production conditions

et al. 2014

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An amylase and lipase producing bacterium (strain C2) was enriched and isolated from soil regularly contaminated with olive washing wastewater in Sfax, Tunisia. Cell was aerobic, mesophilic, Gram-negative, motile, non-sporulating bacterium, capable of growing optimally at pH 7 and 30°C and tolerated maximally 10% (W/V) NaCl. The predominant fatty acids were found to be C18:1ω7c (32.8%), C16:1ω7c (27.3%) and C16:0 (23.1%). Phylogenetic analysis of the 16S rRNA gene revealed that this strain belonging to the genus Pseudomonas. Strain C2 was found to be closely related to Pseudomonas luteola with more than 99% of similarity. Amylase optimization extraction was carried out using Box Behnken Design (BBD). Its maximal activity was found when the pH and temperature ranged from 5.5 to 6.5 and from 33 to 37°C, respectively. Under these conditions, amylase activity was found to be about 9.48 U/ml.

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“…Subsequently, the sample was taken by immersing copper grids coated with a carbon film for 20 min in the bacterial suspensions and washed for 5 min in distilled water and dried for three hours at room temperature (36). as electron donor and sulfate (20 mM) as previously described (43). Temperatures for growth were tested between 4 and 80 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Elemental sulfur (1%, w/v), sulfate (20 mM), sulfite (20 mM), thiosulfate (20 mM), nitrate (10 mM) and nitrite (10 mM) were tested as terminal electron acceptors. The temperature, pH and NaCl concentration ranges for the growth of P. cashew SRB007 were determined in duplicate experiments using autotrophic medium supplemented with lactate (20 mM) as electron donor and sulfate (20 mM) as previously described (43). Temperatures for growth were tested between 4 and 80 °C.…”

Section: Methodsmentioning

confidence: 99%

Pseudodesulfovibrio cashewsp. nov., a novel deep-sea sulfate-reducing bacterium, linking heavy metal resistance and sulfur cycle

Zheng

Sun

2020

Preprint

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Sulfur cycling is primarily driven by sulfate reduction mediated by sulfate-reducing bacteria (SRB) in marine sediments. The dissimilatory sulfate reduction drives the production of enormous quantities of reduced sulfide and thereby the formation of highly insoluble metal sulfides in marine sediments. Here, a novel sulfate-reducing bacterium designated Pseudodesulfovibrio cashew SRB007 was isolated and purified from the deep-sea cold seep and proposed to represent a novel species in the genus of Pseudodesulfovibrio. A detailed description of the phenotypic traits, phylogenetic status and central metabolisms of strain SRB007, allowing the reconstruction of the metabolic potential and lifestyle of a novel member of deep-sea SRB. Notably, P. cashew SRB007 showed a strong ability to resist and remove different heavy metal ions including Fe3+, Co2+, Ni2+, Cu2+, Cd2+ and Hg2+. And the dissimilatory sulfite reduction was demonstrated to contribute to the prominent removal capability of P. cashew SRB007 against different heavy metals via forming insoluble metal sulfides.

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Desulfovibrio tunisiensis sp. nov., a novel weakly halotolerant, sulfate-reducing bacterium isolated from exhaust water of a Tunisian oil refinery

Cited by 35 publications

References 37 publications

Proposal to reclassify the proteobacterial classes Deltaproteobacteria and Oligoflexia, and the phylum Thermodesulfobacteria into four phyla reflecting major functional capabilities

Proposal to reclassify the proteobacterial classes Deltaproteobacteria and Oligoflexia, and the phylum Thermodesulfobacteria into four phyla reflecting major functional capabilities

Isolation of a novel amylase and lipase-producing Pseudomonas luteola strain: study of amylase production conditions

Pseudodesulfovibrio cashewsp. nov., a novel deep-sea sulfate-reducing bacterium, linking heavy metal resistance and sulfur cycle

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