Detailed analysis of hydrocarbon groups in diesel range petroleum fractions with on-line coupled supercritical fluid chromatography–gas chromatography–mass spectrometry

Pál, Róbert; Juhász, Miklós; Stumpf, Árpád

doi:10.1016/s0021-9673(98)00505-6

Cited by 16 publications

(2 citation statements)

References 17 publications

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“…Petroleum diesel oil is a complex mixture of hydrocarbons that typically contain between 8 and 21 carbon atoms per molecule. The organic composition of diesel includes n-alkanes, branched alkanes, saturated cycloalkanes, PAHs, alkylated PAHs and alkylbenzenes which account for 27.90%, 53.87%, 7.72%, 0.26%, 3.70% and 6.55%, respectively, to its composition (Pál et al 1998). Based on the ability of the P39a exopolymer to effectively emulsify a range of individual hydrocarbon species (Fig.…”

Section: Discussionmentioning

confidence: 99%

Production and characterisation of a marine Halomonas surface-active exopolymer

Gutiérrez

Morris

Ellis

et al. 2019

Appl Microbiol Biotechnol

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During screening for novel emulsifiers and surfactants, a marine gammaproteobacterium, Halomonas sp. MCTG39a, was isolated and selected for its production of an extracellular emulsifying agent, P39a. This polymer was produced by the new isolate during growth in a modified Zobell's 2216 medium amended with 1% glucose, and was extractable by cold ethanol precipitation. Chemical, chromatographic and nuclear magnetic resonance spectroscopic analysis confirmed P39a to be a high-molecularweight (~261,000 g/mol) glycoprotein composed of carbohydrate (17.2%) and protein (36.4%). The polymer exhibited high emulsifying activities against a range of oil substrates that included straight-chain aliphatics, mono-and alkyl-aromatics and cycloparaffins. In general, higher emulsification values were measured under low (0.1 M PBS) compared to high (synthetic seawater) ionic strength conditions, indicating that low ionic strength is more favourable for emulsification by the P39a polymer. However, as observed with other bacterial emulsifying agents, the polymer emulsified some aromatic hydrocarbon species, as well as refined and crude oils, more effectively under high ionic strength conditions, which we posit could be due to steric adsorption to these substrates as may be conferred by the protein fraction of the polymer. Furthermore, the polymer effected a positive influence on the degradation of phenanthrene by other marine bacteria, such as the specialist PAH-degrader Polycyclovorans algicola. Collectively, based on the ability of this Halomonas high-molecular-weight glycoprotein to emulsify a range of pure hydrocarbon species, as well as refined and crude oils, it shows promise for the bioremediation of contaminated sites.

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Section: Discussionmentioning

confidence: 99%

Production and characterisation of a marine Halomonas surface-active exopolymer

Gutiérrez

Morris

Ellis

et al. 2019

Appl Microbiol Biotechnol

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“…Combined methods such as: HPLC-GC, GC-GC, GC×GC (two-dimensional GC), or supercritical fluid chromatogra-phy (SFC) are commonly used to improve the quality of analysis. Pál et al (1998) used SFC-GC/MS for de-tailed analysis of different hydrocarbon groups in the range of petroleum fractions and Mao et al (2009) estimated eco-toxicity of petroleum hydrocarbon mixtures in soil using HPLC-GC×GC. All these analytical methods have been modified in order to eliminate ma-trix interferences, reduce the amount of solvent used, find alternative and less toxic solvents, simplify ana-lytical procedures amongst other requirements.…”

Section: Introductionmentioning

confidence: 99%

Determination of total petroleum hydrocarbons in soil from different locations using infrared spectrophotometry and gas chromatography

Paíga

Mendes²,

Albergaria

et al. 2012

Chemical Papers

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Total petroleum hydrocarbons (TPH) are important environmental contaminants which are toxic to human and environmental receptors. Several analytical methods have been used to quantify TPH levels in contaminated soils, specifically through infrared spectrometry (IR) and gas chromatogra-phy (GC). Despite being two of the most used techniques, some issues remain that have been inadequately studied: a) applicability of both techniques to soils contaminated with two distinct types of fuel (petrol and diesel), b) influence of the soil natural organic matter content on the results achieved by various analytical methods, and c) evaluation of the performance of both techniques in analyses of soils with different levels of contamination (presumably non-contaminated and po-tentially contaminated). The main objectives of this work were to answer these questions and to provide more complete information about the potentials and limitations of GC and IR techniques. The results led us to the following conclusions: a) IR analysis of soils contaminated with petrol is not suitable due to volatilisation losses, b) there is a significant influence of organic matter in IR analysis, and c) both techniques demonstrated the capacity to accurately quantify TPH in soils, irrespective of their contamination levels.

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Supercritical Fluid Chromatography

Yuan

Olesik

2000

Encyclopedia of Analytical Chemistry

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Supercritical fluid chromatography (SFC) employs fluids that are raised above their critical pressure (P c ) and critical temperature (T c ) as mobile phases. Carbon dioxide, CO 2 , is the supercritical fluid most commonly used as a mobile phase for SFC. It is capable of solvating mostly nonpolar analytes. Cosolvents, such as methanol and acetonitrile, are added toincrease the polarity of the analytes that can be analyzed by SFC. SFC is most commonly practiced using a normal‐phase retention mechanism. In other words, the stationary phase is typically more polar than the mobile phase and the retention order is in increasing order of polarity. The advantages of SFC over liquid chromatography (LC) include higher efficiency separations and faster speed of analysis. The advantage that SFC has over gas chromatography (GC) is that SFC can efficiently separate thermally labile compounds. The disadvantage of SFC is that the polarity of the mobile phase is limited. Highly polar solutes are not soluble in supercritical fluids, even when modifiers are added. SFC is commonly used to separate petroleum distillates and by‐products, moderately polar pesticides and herbicides, and more recently for the separation of chiral compounds, such as pharmaceutical drugs.

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Detailed analysis of hydrocarbon groups in diesel range petroleum fractions with on-line coupled supercritical fluid chromatography–gas chromatography–mass spectrometry

Cited by 16 publications

References 17 publications

Production and characterisation of a marine Halomonas surface-active exopolymer

Production and characterisation of a marine Halomonas surface-active exopolymer

Determination of total petroleum hydrocarbons in soil from different locations using infrared spectrophotometry and gas chromatography

Supercritical Fluid Chromatography

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