Detailed analysis of the fatty acid composition of six plant-pathogenic bacteria

Wiedmaier‐Czerny, Nina; Schroth, Dorothee; Topman-Rakover, Shiri; Brill, Aya; Burdman, Saul; Hayouka, Zvi; Vetter, Walter

doi:10.1016/j.jchromb.2020.122454

Cited by 7 publications

(10 citation statements)

References 40 publications

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“…A bacterial fatty acid (BFA) input is usually represented by a combination of branched fatty acids, transfatty acids, odd-carbon number fatty acids, and nC14:1, nC16:1, nC18:1, etc. unsaturated fatty acids (Kaneda 1991, Mosto an et al 2019, Parkes et al 1993, Sushchik et al 2007, Volkman 1986, Wiedmaier-Czerny et al 2021, Zou et al 2004) .…”

Section: Discussionmentioning

confidence: 99%

Origin and Factors Effecting Dissolved Organic Matter in Karst Spring, Southwest China

Liao

Shen

Sun

et al. 2021

Preprint

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The dissolved biomarker composition of water samples was detected and quantified through gas chromatography-mass spectrometry to investigate the source of organic matter and its influencing factors in a subtropical monsoon karst spring. All water samples were collected in Chongqing, southwest China, in May, June, and July 2015. An analysis of the carbon number distribution characteristics and molecular characteristic parameters indicated that the dissolved organic matter in water samples mainly came from microorganism. The average biomarker contents in the samples were controlled by multi-factor coupling and showed an increasing trend in May, June, and July; this trend was consistent with temperature and inverse with rainfall. Human activities, such as urbanization and land use type, affected the fatty acid composition of the spring water. In forested land, the main fatty acids in water were saturated normal fatty acids with carbon numbers of 14,16, and 18, respectively. The C16:1ω9 (c) fatty acid, denoting the origin of algae, was found in high abundance in the water samples of the Mudu River Basin, which contains agricultural land far from urban areas. C18:1 ω9 (t), a biomarker of Gram-negative bacteria, was detected in large quantities in the cultivated land of the Laolongdong watershed.

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Section: Discussionmentioning

confidence: 99%

Origin and Factors Effecting Dissolved Organic Matter in Karst Spring, Southwest China

Liao

Shen

Sun

et al. 2021

Preprint

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“…Fatty acid patterns of untreated strains of six bacteria (controls, Table 1 ) were recently studied by Wiedmaier-Czerny et al 23 . In brief, cultivation was performed using existing standard protocols at the Institute of Biochemistry, Food Science and Nutrition (Hebrew University of Jerusalem, Israel) 9 .…”

Section: Methodsmentioning

confidence: 99%

“…Fatty acids present in about 10 mg of freeze-dried bacteria (pellet) or about 100 mg of freeze-dried supernatant were converted into the corresponding methyl esters (FAMEs) according to Wendlinger et al 22 with slight modifications 23 . In short, 2 mL of 1% sulfuric acid in methanol was added and the samples were heated first for 90 min to 80 °C, then sonicated for 10 min and finally heated again for 30 min to 80 °C.…”

Section: Methodsmentioning

confidence: 99%

“…The final concentration for measuring was ~ 70 µg FAMEs per mL n -hexane. Internal standard (ISTD) solution (10 µL of 100 µg/mL 11:0 ethyl ester (11:0-EE) for 1 mL, prepared according to Wiedmaier-Czerny et al 23 ) was added to each sample vial. Two initially measured samples (“first treatment”, samples labelled with “ p ” in subscript, namely X. campestris p , A. citrulli p ) were extracted by accelerated solvent extraction (ASE) by three different solvents 23 .…”

Section: Methodsmentioning

confidence: 99%

“…Then, lipids were extracted from freeze-dried samples, transesterified and the resulting fatty acid methyl esters (FAMEs) were analysed by gas chromatography coupled with mass spectrometry (GC/MS) 21 , 22 . Fatty acid patterns were related to those of the (untreated) control samples 23 . Evaluation was mainly based on four groups of FA, i.e.…”

Section: Introductionmentioning

confidence: 99%

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Stable isotope analysis confirms substantial changes in the fatty acid composition of bacteria treated with antimicrobial random peptide mixtures (RPMs)

Wiedmaier‐Czerny

Schroth

Krauß

et al. 2022

Sci Rep

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Resistance of plant-pathogenic bacteria to classic antibiotics has prompted the search for suitable alternative antimicrobial substances. One promising strategy could be the use of purposely synthesized random peptide mixtures (RPMs). Six plant-pathogenic bacteria were cultivated and treated with two RPMs previously found to show antimicrobial activity mainly by bacterial membrane disruption. Here, we show that bacteria treated with RPMs showed partly remarkable changes in the fatty acid pattern while those unaffected did not. Quantitative changes could be verified by compound specific isotope analysis of δ13C values (‰). This technique was employed due to the characteristic feature of stronger bonds between heavier isotopes in (bio)chemical reactions. As a proof of concept, the increase in abundance of a fatty acid group after RPM treatment was accompanied with a decrease in the 13C content and vice versa. We propose that our findings will help designing and synthesizing more selective antimicrobial peptides.

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LC–MS/MS-based phospholipid profiling of plant-pathogenic bacteria with tailored separation of methyl-branched species

Rudt,

Faist,

Schwantes

et al. 2024

Anal Bioanal Chem

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Plant-pathogenic bacteria are one of the major constraints on agricultural yield. In order to selectively treat these bacteria, it is essential to understand the molecular structure of their cell membrane. Previous studies have focused on analyzing hydrolyzed fatty acids (FA) due to the complexity of bacterial membrane lipids. These studies have highlighted the occurrence of branched-chain fatty acids (BCFA) alongside normal-chain fatty acids (NCFA) in many bacteria. As several FA are bound in the intact phospholipids of the bacterial membrane, the presence of isomeric FA complicates lipid analysis. Furthermore, commercially available reference standards do not fully cover potential lipid isomers. To address this issue, we have developed a reversed-phase high-performance liquid chromatography (RP-HPLC) method with tandem mass spectrometry (MS/MS) to analyze the phospholipids of various plant-pathogenic bacteria with a focus on BCFA containing phospholipids. The study revealed the separation of three isomeric phosphatidylethanolamines (PE) depending on the number of bound BCFA to NCFA. The validation of the retention order was based on available reference standards in combination with the analysis of hydrolyzed fatty acids through gas chromatography with mass spectrometry (GC/MS) after fractionation. Additionally, the transferability of the retention order to other major lipid classes, such as phosphatidylglycerols (PG) and cardiolipins (CL), was thoroughly examined. Using the information regarding the retention behavior, the phospholipid profile of six plant-pathogenic bacteria was structurally elucidated. Furthermore, the developed LC–MS/MS method was used to classify the plant-pathogenic bacteria based on the number of bound BCFA in the phospholipidome. Graphical Abstract

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Detailed analysis of the fatty acid composition of six plant-pathogenic bacteria

Cited by 7 publications

References 40 publications

Origin and Factors Effecting Dissolved Organic Matter in Karst Spring, Southwest China

Origin and Factors Effecting Dissolved Organic Matter in Karst Spring, Southwest China

Stable isotope analysis confirms substantial changes in the fatty acid composition of bacteria treated with antimicrobial random peptide mixtures (RPMs)

LC–MS/MS-based phospholipid profiling of plant-pathogenic bacteria with tailored separation of methyl-branched species

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