Detailed characterization of an IncFII plasmid carrying blaOXA-48 from Lebanon

Abstract

Background The spread of carbapenem-resistant Enterobacteriaceae is an important challenge and an increasing healthcare problem. OXA-48 is a class D carbapenemase that is usually localized on a conjugative plasmid belonging to the IncL incompatibility group. Methods In this study, we used a combination of short- and long-read WGS approaches and molecular typing techniques to characterize the genetic environment of the smallest reported 27 029 bp IncFII plasmid carrying blaOXA-48 (pLAU-OXA48). Results The plasmid recovered from a clinical Escherichia coli isolate was positive for blaOXA-48, which was located within the Tn6237 composite transposon. Primers targeting junctions between the IncF fragment and Tn6237 for the rapid identification of pLAU-OXA48-like plasmids were designed. Conclusions To our knowledge, this is the first report showing the complete sequence of an IncFII plasmid carrying blaOXA-48 within Tn6237 using hybrid assembly of long- and short-read sequencing.

Klebsiella Species and Enterobacter cloacae Isolates Harboring bla OXA-181 and bla OXA-48 : Resistome, Fitness Cost, and Plasmid Stability

“…Plasmid-borne AmpC genes usually lack genetic components required for regulation of AmpC expression, and therefore expression results from continuous transcription of the gene. Hence, as a β-lactamase that is inducible, regulation of its expression involves one of the genes ampR , which encodes a transcriptional regulator of the LysR family, ampG , which encodes a transmembrane permease, and ampD , which encodes cytosolic N -acetyl-anhydromuramyl- l -alanine amidase ( 31 , 32 ). This explains the presence of LysR immediately upstream of bla DHA-1 , as found in the 22-kb structure.…”

bla OXA-48-like genome architecture among carbapenemase-producing Escherichia coli and Klebsiella pneumoniae in the Netherlands

“…This suggests the presence of a potentially recently introduced set of plasmids that have not yet widely spread in the Netherlands. OXA-48 plasmids with either an IncR, IncFII or IncY replicon have only recently been described and are relatively rare [30–32]. The presence of these variable and rare bla OXA-48 -like plasmids suggest that the current OXA-48 plasmid reservoir may larger than currently reported.…”

Four Types of ST11 Novel Mutations From Increasing Carbapenem-Resistant Klebsiella pneumoniae in Guangdong, 2016–2020

“…Thus, strains that easily cause outbreaks should be given attention in Guangdong. Several studies show that OXA has gradually increased in CR-KP (Pitout et al, 2019;Moussa et al, 2020), while all ST45, ST37, and ST76 isolates contained OXA. This finding is worrisome considering that ST45, ST76, and ST37 can potentially cause outbreaks of hospital infection.…”