Detam 1 black soybean against cisplatin-induced acute ren failure on rat model via antioxidant, antiinflammatory and antiapoptosis potential

Widowati, Wahyu; Prahastuti, Sijani; Hidayat, Meilinah; Hasianna, Stella Tinia; Wahyudianingsih, Roro; Eltania, The Fransiska; Azizah, Alya Mardhotillah; Aviani, Jenifer Kiem; Subangkit, Mawar; Handayani, Rr. Anisa Siwianti; Kusuma, Hanna Sari Widya

doi:10.1016/j.jtcme.2022.01.004

Cited by 14 publications

(21 citation statements)

References 54 publications

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“…Bovine standard albumin at different concentrations and 200 mL of quick start dye reagent 1X (Bio-Rad, 5000205, USA) were added to the well along with 20 mL of sample. The plate was incubated for 5 min at room temperature and the samples’ absorbance was detected at 595 nm (Multiskan GO Microplate spectrophotometer, Thermo Fisher Scientific, USA) ( 26 27 ).…”

Section: Methodsmentioning

confidence: 99%

Protective effect of yacon leaves extract (Smallanthus sonchifolius (Poepp.) H. Rob) through antifibrosis, anti-inflammatory, and antioxidant mechanisms toward diabetic nephropathy

Widowati¹,

Tjokropranoto²,

Onggowidjaja³

et al. 2023

Research in Pharmaceutical Sciences

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Background and purpose: Diabetic nephropathy (DN) is a chronic kidney failure, which may lead to fatality. Mesangial cell proliferation, renal inflammation, stress oxidative, and fibrosis are involved in DN progression. Yacon leaves ( Smallanthus sonchifolius (Poepp.) H. Rob.) contains large amounts of phenolic compounds and it has the ability to inhibit oxidative stress, inflammation, and fibrosis. Considering the potential of yacon leaves extract (YLE), it may be used for DN treatment. This research aimed to elucidate YLE’s potential as anti-DN through anti-inflammatory, antioxidant, and antifibrosis mechanisms. Experimental approach: Mesangial cells were induced by glucose 20 mM for 5 days and treated with YLE concentrations as much as 5, 10, and 50 µg/mL. TGF-β1, TNF-α, and MDA levels were measured using the ELISA method. SMAD2, SMAD3, SMAD4, and SMAD7 gene expressions were analyzed using the qRT-PCR method. Findings/Results: YLE at 5, 10, and 50 µg/mL could reduce the levels of TGF-β1, TNF-α, and MDA compared with the DN cells model. YLE could reduce gene expressions of SMAD2, SMAD3, and SMAD4 and increase SMAD7 expression. Conclusion and implications: YLE potentially mitigated diabetic nephropathy through antifibrosis, anti-inflammatory, and antioxidant capacities.

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Section: Methodsmentioning

confidence: 99%

Protective effect of yacon leaves extract (Smallanthus sonchifolius (Poepp.) H. Rob) through antifibrosis, anti-inflammatory, and antioxidant mechanisms toward diabetic nephropathy

Widowati¹,

Tjokropranoto²,

Onggowidjaja³

et al. 2023

Research in Pharmaceutical Sciences

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“…Epigallocatechin, catechin, and epicatechin gallate [102] ↓ pERK1/2 phosphorylation ↓ oxLDL plasma levels [103] Catechin, epicatechin, quercetin, caffeic acid, and ferulic acid [107] ↓ urinary NAG activity ↓ renal histopathological lesion ↓ urea and serum creatinine [107] Ferulic acid ↓ serum BUN and creatinine levels ↓ urinary albumin concentration and urinary NAG activity ↑ CAT activity ↑ RvE1 concentration ↑ PPAR-γ gene expression [108] Ferulic acid [110] Prevented disruption of the normal renal architecture ↓ creatinine, BUN, LDH, TNFa, and KIM-1 levels [109] Gallic acid, chlorogenic acid, syringic acid, (+)-catechin, (β)-epicatechin, and rutin [112] ↓ MDA, Hyp, and Hcy [111] P-couramic, caffeic, and ferulic acids [114] ↓ serum MDA, lipid peroxidation ↑ plasma catalase and total antioxidant capacity ↑ Cr ↓ chromosomal aberrations in bone marrow cells and sperm shape abnormalities [113] Betacyanins [115] ↓ plasma creatinine, BUN, and NGAL ↓ tubular damage ↑ MDA levels ↓ Nrf2 levels ↑ NO 2 − /NO 3 − levels [115] Isoflavones (daidzein, daidzin, genistin, biochanin A, and glycitein) [116] ↓ cytoplasmic IFN-γ expression ↓ Casp-3 expression ↓ IL-6, IL-1b, TGF-b1, TLR-4, F4/80 and TNF-a in kidneys ↓ BUN and UA ↑ CAT [116] Flavonoids and terpenoids [117] ↓ blood urea and creatinine ↑ glutathione peroxidase activity [117] Table 1. Cont.…”

Section: Compound Reported Bioactivitymentioning

confidence: 99%

Exploring the Role of Phenolic Compounds in Chronic Kidney Disease: A Systematic Review

Baptista,

Paié-Ribeiro,

Almeida

et al. 2024

Molecules

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Chronic kidney disease (CKD) presents a formidable global health concern, affecting one in six adults over 25. This review explores the potential of phenolic compounds in managing CKD and its complications. By examining the existing research, we highlight their diverse biological activities and potential to combat CKD-related issues. We analyze the nutritional benefits, bioavailability, and safety profile of these compounds. While the clinical evidence is promising, preclinical studies offer valuable insights into underlying mechanisms, optimal dosages, and potential side effects. Further research is crucial to validate the therapeutic efficacy of phenolic compounds for CKD. We advocate for continued exploration of their innovative applications in food, pharmaceuticals, and nutraceuticals. This review aims to catalyze the scientific community's efforts to leverage phenolic compounds against CKD-related challenges.

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“…GTE compound contents were analyzed using LC-MS method with Hypersil Gold specifications (150 mm × 2.1 mm × 1.9 µm). TSQ Quantum Access MS/MS Triple Q (quadrupole) mass spectrometer with Electrospray Ionization (ESI) was controlled by TSQ Tune software, which operated with a positive charge [22].…”

Section: Lc-ms/ms Analysismentioning

confidence: 99%

“…PCR included priming at 25 °C for 5 min, reverse transcription at 46 °C for 20 min, RT inactivation at 95 °C for 1 min, and optimization step at 4 °C. After that, for gene expression analysis of ACE-2 and TMPRSS-2 were carried out used SYBR NO-ROX kit (Bioline, BIO-98005) and primer [22]. The primer sequences were shown in Table 1.…”

Section: Quantification Of Ace-2 and Tmprss-2 Genes Expressionmentioning

confidence: 99%

Camellia sinensis L. Extract Suppresses Inflammation on Acute Respiratory Distress Syndrome Cells Models via Decreasing IL-1ß, IL-6 and COX-2 Expressions

Widowati,

Priyandoko,

Lenny

et al. 2023

Trends Sci

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Acute Respiratory Distress Syndrome (ARDS) is one of the clinical manifestations in severe COVID-19 patients characterized by acute inflammation resulting in respiratory failure and death. Camellia sinensis L. or green tea extract has many beneficial secondary metabolites including polyphenols which have anti-inflammatory and anti-viral roles. This study was aimed to investigate the activity of green tea extract (GTE) as an anti-inflammatory on ARDS model cells. In this study, rat lung alveolar type II epithelial cells line (L2) was induced by lipopolysaccharide (LPS) to mimic the inflammation process in ARDS. These cells were then treated with GTE to determine the GTE effectiveness in reducing the inflammation. The GTE polyphenol constitutions were first confirmed using Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS). The cytotoxic assay was conducted using MTS assay kit to determine the safe range of GTE concentrations that used in the next assay. The effectiveness of the GTE was determined by measuring pro-inflammatory cytokines (IL-1ß and IL-6) and inflammation-mediated enzymes (COX-2), both of which were by ELISA method. Furthermore, this study measured the ACE-2 and TMPRSS-2 gene expressions by qRT-PCR method. The results showed that GTE treatment significantly reduced pro-inflammatory cytokines IL-1ß, IL-6, inflammation-mediated enzyme COX-2, and significantly increased ACE-2 and TMPRSS-2 mRNA expressions. In short, green tea extract possesses the potential to alleviate inflammation. HIGHLIGHTS Acute Respiratory Distress Syndrome (ARDS) is one of clinical manifestations in severe COVID-19 patients characterized by acute inflammation results in respiratory failure and death In lung inflammation, the Angiotensin Converting Enzyme-2 (ACE-2) and Trans-Membrane Protease Serine-2 (TMPRSS-2) genes were lowly expressed, then causes alveolar tissue collapse High levels of inflammatory mediator cytokines would be secreted in respiratory tracts, such as Interleukin (IL)-1β, IL-6, and Cyclooxygenases (COX)-2 Polyphenols in green tea (Camelia sinensis L.) have been reported to have anti-inflammatory activities GRAPHICAL ABSTRACT

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Detam 1 black soybean against cisplatin-induced acute ren failure on rat model via antioxidant, antiinflammatory and antiapoptosis potential

Cited by 14 publications

References 54 publications

Protective effect of yacon leaves extract (Smallanthus sonchifolius (Poepp.) H. Rob) through antifibrosis, anti-inflammatory, and antioxidant mechanisms toward diabetic nephropathy

Protective effect of yacon leaves extract (Smallanthus sonchifolius (Poepp.) H. Rob) through antifibrosis, anti-inflammatory, and antioxidant mechanisms toward diabetic nephropathy

Exploring the Role of Phenolic Compounds in Chronic Kidney Disease: A Systematic Review

Camellia sinensis L. Extract Suppresses Inflammation on Acute Respiratory Distress Syndrome Cells Models via Decreasing IL-1ß, IL-6 and COX-2 Expressions

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