Detecção de fungos endofíticos em sementes de caupi provenientes de Serra Talhada e de Caruaru, estado de Pernambuco

Rodrigues, Antônia Alice Costa; Menezes, Maria

doi:10.1590/s0100-41582002000500016

Cited by 13 publications

(14 citation statements)

References 3 publications

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“…). Different Colletotrichum species have been found in association with endophytic fungal communities in a large variety of plants from different ecosystems, such as Vigna unguiculata (Rodrigues and Menezes ), Taxus mairei (Wang et al . ), Coffea arabica (Fernandes et al .…”

Section: Introductionmentioning

confidence: 99%

“…), coffee (Fernandes et al . ), cowpea (Rodrigues and Menezes ), corn (Orole and Adejumo ) and soya bean (Pimentel et al . ; Hamayun et al .…”

Section: Introductionmentioning

confidence: 99%

“…However, phytopathogenic species from this genus are capable of mutualistic or commensal lifestyles on plants other than those in which they cause disease (Redman et al 2001). Different Colletotrichum species have been found in association with endophytic fungal communities in a large variety of plants from different ecosystems, such as Vigna unguiculata (Rodrigues and Menezes 2002), Taxus mairei (Wang et al 2008), Coffea arabica (Fernandes et al 2009), Camptotheca acuminata (Ding et al 2010) and Jatropha curcas (Kumar and Kaushik 2013).…”

Section: Introductionmentioning

confidence: 99%

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Endophytic fungi from the genusColletotrichumare abundant in thePhaseolus vulgarisand have high genetic diversity

et al. 2014

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Section: Introductionmentioning

confidence: 99%

“…), coffee (Fernandes et al . ), cowpea (Rodrigues and Menezes ), corn (Orole and Adejumo ) and soya bean (Pimentel et al . ; Hamayun et al .…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Endophytic fungi from the genusColletotrichumare abundant in thePhaseolus vulgarisand have high genetic diversity

et al. 2014

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“…and Penicillium spp. affecting germination, provoking necrosis of the cotyledons, radicle and initial leaves of cowpea, resulting in abnormal seedlings (Rodrigues & Menezes, 2002). When high incidences of these species are found they may make germination inviable and cause fungal structures to grow on soybean seeds .…”

Section: Resultsmentioning

confidence: 99%

Fungal Diversity in Lima Bean Seeds

Mota

Melo

Silva

et al. 2017

Revista Brasileira De Engenharia De Biossistemas

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There is no comprehensive survey of the presence of fungi associated with lima bean seeds. The objective of this study was to evaluate the fungal diversity of 34 samples of lima bean seeds, acquired with farmers and markets during the years 2014 and 2015 in the states of Ceará, Maranhão, Paraíba and Piauí. Subsamples of 400 seeds were sterilized by soaking in 1% NaOCl solution for 3 minutes, followed by two washes in sterile distilled water, and dried at room temperature. Seeds were placed in Petri plates containing Potato Dextrose Agar, and incubated at 25 C for seven days. Fungal identification was based on morphological markers, and its incidences were quantified. Isolates from the main phytopathogens were also identified by the amplification and sequencing of housekeeping genes. Samples presented variations in diversity and incidence, with the presence of 22 fungal genera. Aspergillus spp., Penicillium spp., Curvularia sp. and Monilinia sp. corresponded to 63.76% of the colonies observed. Among the phytopathogens, isolates of Macrophomina phaseolina, Colletotrichum truncatum, Rhizoctonia solani, Fusarium udum and Fusarium oxysporum were identified by BLASTn analysis (99 to 100% DNA similarity) and phylogenetic analysis. C. truncatum and M. phaseolina presented the highest incidences (0.95% and 1.58%, respectively) among phytopathogens. Keywords: Colletotrichum truncatum, Macrophomina phaseolina, Phaseolus lunatus, seed pathology DIVERSIDADE FÚNGICA EM SEMENTES DE FEIJÃO-FAVA RESUMONão existe levantamento abrangente da presença de fungos associados a sementes de feijão-fava. O objetivo deste trabalho foi avaliar a diversidade fúngica de 34 amostras de sementes de feijão-fava, adquiridas com produtores rurais e em feiras livres, durante os anos de 2014 e 2015, nos estados do Ceará, Maranhão, Paraíba e Piauí. Subamostras de 400 sementes foram desinfestadas, e incubadas a 25 C durante sete dias em placas de Petri contendo meio de cultura Batata-Dextrose-Ágar. A identificação dos fungos foi realizada com base nos marcadores morfológicos, e suas incidências foram quantificadas. Isolados dos principais fitopatogógenos também foram identificados pela amplificação e sequenciamento de genes "housekeeping". As amostras apresentaram variações na diversidade e incidência, com a presença de 22 gêneros fúngicos. Aspergillus spp., Penicillium spp., Curvularia sp. e Monilinia sp. corresponderam a 63,76% das colônias observadas. Dentre os fitopatógenos, os isolados de Macrophomina phaseolina, Colletotrichum truncatum, Rhizoctonia solani,Brazilian Journal of Biosystems Engineering v. 11(1): 79-87, 2017 80 Fusarium udum e Fusarium oxysporum foram identificados por análise BLASTn (99 a 100% de similaridade de DNA) e análise filogenética. C. truncatum e M. phaseolina apresentaram as maiores incidências (0,95% e 1,58%, respectivamente) dentre os fitopatógenos.

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“…Essa cultura apresenta alguns entraves, entre os quais destaca-se a falta de adoção de tecnologia, principalmente em relação aos insumos, onde o agricultor recorre ao uso de sementes não selecionadas, contribuindo para o surgimento de doenças (RODRIGUES & MENEZES, 2002).…”

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Efeito de extratos vegetais no controle de Fusarium oxysporum f. sp tracheiphilum em sementes de caupi

Silva

Pegado²,

Ribeiro

et al. 2009

Ciênc. agrotec.

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, Luciana Cordeiro do Nascimento RESUMOA fusariose é uma doença de grande importância para a cultura do feijoeiro em virtude da redução da produtividade. O controle da doença vem sendo realizado com aplicações indiscriminadas de fungicidas químicos, acarretando problemas ao homem e ao meio ambiente. Objetivou-se, no presente trabalho, avaliar o efeito de extratos vegetais no controle de Fusarium oxysporum f. sp tracheiphilum, proveniente de sementes de caupi, comparando-se com o efeito do fungicida químico. Utilizaram-se extratos de alho, angico e manjericão, isolados ou combinados entre si e ou em associação ao fungicida Mancozeb. Uma alíquota de 50 µL de cada tratamento foi adicionada em orifício feito no centro das placas de Petri com BDA, sobre o qual depositou-se um disco de micélio do fungo. Avaliou-se durante sete dias, medindo-se o crescimento micelial do patógeno. Sementes de caupi foram desinfestadas com hipoclorito de sódio a 4,0%, semeadas em sacos de polietileno contendo solo autoclavado. Oito dias após germinação, efetuaram-se ferimentos no colo das plântulas, aplicando-se suspensão de esporos do fungo (1,4 x 10 -5 con/mL). As avaliações de severidade da doença foram realizadas diariamente durante 30 dias após a inoculação, utilizando-se escala de notas. Os extratos combinados de alho + manjericão e angico + manjericão não inibiram o crescimento micelial do fungo, enquanto o extrato de manjericão isoladamente, proporcionou o menor crescimento micelial, indicando a ação fungicida e inibitória desse tratamento sobre o fungo F. oxysporum f. sp tracheiphilum nas condições analisadas. O fungicida Mancozeb + o extrato de angico proporcionou menor média de severidade nas plantas avaliadas.Termos para indexação: Vigna unguiculata, controle alternativo, fusariose. ABSTRACTWilt caused by Fusarium sp. in cowpea crop is a disease of great importance because it causes yield decrease. The control of this disease has been accomplished with uncontrolled applications of chemical fungicides causing problems for humans and environment. The present work had the objective to compare the effect of natural extracts on the control of Fusarium oxysporum f. sp tracheiphlum, originated from seeds of cowpea (Vigna unguiculata L), compared to chemical fungicide effect. Extracts of Allium sativum, Anadenanthera colubrine, and Ocimum basilicum were used alone or in combination with Mancozeb. A 50 µL aliquot of each treatment was added in a hole (6 mm of diameter) made in the center of Petri dishes with BDA and a fungus mycelia disk was inoculated on it. Evaluations were done during seven days, measuring the pathogen mycelial growth. Seeds of cowpea were disinfected with sodium hypochlorite, sowed in plastic bags with autoclaved soil. Eight days after germination, wounds were made on the basal part of the plantlets, and fungus spore suspension (1.4 x 10 -5 con/mL) was applied on it. Evaluations of the disease severity were accomplished daily during 30 days after inoculation, using a disease index. Combination of extracts of A. sa...

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Detecção de fungos endofíticos em sementes de caupi provenientes de Serra Talhada e de Caruaru, estado de Pernambuco

Cited by 13 publications

References 3 publications

Endophytic fungi from the genusColletotrichumare abundant in thePhaseolus vulgarisand have high genetic diversity

Endophytic fungi from the genusColletotrichumare abundant in thePhaseolus vulgarisand have high genetic diversity

Fungal Diversity in Lima Bean Seeds

Efeito de extratos vegetais no controle de Fusarium oxysporum f. sp tracheiphilum em sementes de caupi

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