Detecção molecular e isolamento de Mycoplasma spp. em psitacídeos no estado de Pernambuco, Brasil

Silva, L.T.R.; Santos, Sandra Batista dos; Rameh-de-Albuquerque, L.C.; Siqueira, Daniel B.; Amorim, Marcus Mello Rego de; Almeida, Jonatas Campos de; Oliveira, Andréa Alice da Fonseca; Mota, R. A.

doi:10.1590/1678-4162-8387

Cited by 6 publications

(4 citation statements)

References 16 publications

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“…The results found for Psittaciformes contrast the studies of Silva et al (2016) and Carvalho et al (2017) when assessing the presence of Mycoplasma spp. in asymptomatic parrots under human care.…”

Section: Resultscontrasting

confidence: 94%

“…in asymptomatic parrots under human care. Silva et al (2016), when analyzing 85 parrots of different species from a zoo in Pernambuco, reported the presence of Mycoplasma spp. in 16.47% of the birds, however these mycoplasmas were not identified as MG or MS. Carvalho et al (2017), when evaluating 300 samples of CETAS parrots, commercial and conservation breeding, observed positivity for M. gallisepticum (MG) in 21.6% (16/74) in CETAS, 15.7% (19/121) in commercial breeding, and 6.7% (7/105) in conservationist, while for MS the occurrences were 2.7 % (2/74) in CETAS, 0.0% (0/121) in commercial breeding and 1.9% (2/105) in conservationist.…”

Section: Resultsmentioning

confidence: 99%

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Investigation of Mycoplasma spp. in birds of the Rio de Janeiro Zoo by isolation and PCR

et al. 2020

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Brazil is one of the countries with the most abundant avifauna in the world. The confinement of birds associated with close contact with other animals and humans favor the spread of agents of respiratory diseases. Among them, mycoplasmas can cause asymptomatic or apparent disease that manifests in birds by coughing, sneezing, rales, conjunctivitis, ocular and nasal discharge. Several described mycoplasmas cause disease in birds, especially Mycoplasma gallisepticum(MG) andMycoplasma synoviae(MS). The diagnosis ofMycoplasmaspp. can be done by clinical observation and laboratory analysis. Molecular diagnosis by PCR was boosted by its speed, sensitivity, and low cost of agent isolation techniques that take up to 21 days to complete. This study aimed to verify the occurrence ofMycoplasmaspp. in birds of the Rio de Janeiro Zoo (Rio Zoo), by isolation and PCR. Of the total 635 birds from the Rio Zoo, 81 were studied for detection ofMycoplasmaspp., when taken for routine health assessment exams. These birds belonged to the following orders: Psittaciformes (45), Accipitriformes (18), Galliformes (7), Piciformes (5), Strigiformes (4), Falconiformes (1) and Cariamiformes (1), all individuals already identified by microchip or leg-ring. There was no isolation of mycoplasmas in any of the samples tested, whereas, in the PCR, 62.96% (51/81) were positive, with 1.96% (1/51) identified as MG and 19.61% (10/51) as MS, representing 1.23% (1/81) and 12.34% (10/81) of the total population studied. PCR was shown to be a more effective technique than isolation in the detection ofMycoplasmaspp. in birds. It was possible to detect mycoplasmas in birds from Riozoo with no clinical respiratory signs, with higher MS prevalence than MG. The positivities forMycoplasmaspp., MS, and MG were different among the orders studied, being the highest occurrence in birds of prey, followed by Galliformes and Piciformes. The presence of MG and MS in birds of Rio de Janeiro Zoo confirms the circulation of these agents and the need for further studies on the dissemination of mycoplasmas in zoos for the epidemiological analysis of these bacteria in these places.

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Section: Resultscontrasting

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Investigation of Mycoplasma spp. in birds of the Rio de Janeiro Zoo by isolation and PCR

et al. 2020

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“…The lack of previous studies in Brazilian psittacine embryos or young, did not allow local comparisons. However, captive psittacines were tested (cloaca, conjunctiva, and palate) in Recife (Pernambuco, Brazil) and shown 6.6% positive for Mycoplasma spp., but none for MG or MS (Silva et al, 2016). These results corroborate with our findings regarding the negativity to MG, although a lower level of infection was detected, possibly related to differences in transmission conditions.…”

Section: Arqsupporting

confidence: 91%

Detection of Mycoplasma in dead psittacine embryos

Mureb,

Medeiros,

Marín

et al. 2023

Arq. Bras. Med. Vet. Zootec.

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Captive breeding of avian species, both for commercial and conservation purposes, demands healthy breeders for viable healthy progenies. Commercially raised pets must be healthy for market viability and considering the conservation projects, sufficient and health progeny numbers are required, especially for the endangered species. Native Brazilian species, endangered or extinct from nature, have been reproduced in captivity and the obtained progenies have enabled their return to nature, as demonstrated for Amazona aestiva (Lopes, 2016), Aburria jacutinga, Crax blumenbachii, and Pauxi mitu in Brazil (Avelar and Simpson, 2014;Silveira, 2017). Both natural and artificial incubation may be impaired by embryonic disease, despite artificial incubation may enable the hygienic control of eggs and the elimination of parental/nest transmission (Smith, 2019). Species of Mycoplasma spp. are amongst agents capable of causing embryonic and hatchling mortality (Kleven, 2008), and although most species were described in chickens and turkeys, 17 species were found in wild avian hosts, including Mycoplasma buteonis, M. corogypsi, M. falconis, M. gypis and M. sturni (Luttrell and Fischer, 2007). However, M. gallisepticum (MG) was isolated from house finches (Carpodacus mexicanus) with conjunctivitis in the surroundings of poultry houses (Ley et al., 1996), which indicated spill over from chickens to passerines. MG, M. synoviae (MS) and M. meleagridis (MM) are long been known for

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“…Difference in culture and PCR results can be explained by the number of mycoplasmas in the sample, occurrence of inhibitors or failure in the diagnostic method (Timenetsky et al, 2006). The presence of mycoplasmas in asymptomatic wild captive birds has already been reported in the study by Silva et al (2016) who observed a prevalence of 16.5% Mycoplasma spp. in 85 parrots and Carvalho et al (2017) who found percentages of 21.6%, 15.7% and 6.7% positivity in birds from, respectively, a Wild Animal Sorting Center, a commercial breeding site and a conservation breeding site and these results agree with what was found in our study.…”

Section: Occurrence Of Avian Mycoplasmas In Free-living Muscovy-ducksmentioning

confidence: 87%

Occurrence of Avian Mycoplasmas in Free-Living Muscovy-Ducks (Cairina Moschata)

Magalhães

Pereira

Machado

et al. 2020

Braz. J. Poult. Sci.

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Detecção molecular e isolamento de Mycoplasma spp. em psitacídeos no estado de Pernambuco, Brasil

Abstract: RESUMOObjetivou-se com este estudo investigar a ocorrência de Mycoplasma spp., Mycoplasma galissepticum (MG)

Cited by 6 publications

References 16 publications

Investigation of Mycoplasma spp. in birds of the Rio de Janeiro Zoo by isolation and PCR

Investigation of Mycoplasma spp. in birds of the Rio de Janeiro Zoo by isolation and PCR

Detection of Mycoplasma in dead psittacine embryos

Occurrence of Avian Mycoplasmas in Free-Living Muscovy-Ducks (Cairina Moschata)

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