Detección de  Chlamydia abortus  en pérdidas reproductivas de bovinos en la provincia de La Pampa, Argentina

Rojas, María del Carmen; Fort, Marcelo; Bettermann, Simone; Entrocassi, Carolina; Costamagna, Sixto Raúl; Sachse, Konrad; Fermepin, Marcelo Rodríguez

doi:10.1016/j.ram.2017.10.002

Cited by 10 publications

(7 citation statements)

References 24 publications

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“…Recently Rojas et al . () detected Chlamydiaceae DNA in 12 samples of 251 bovine aborted fetuses (4.78%) and C. abortus was detected in five of the studied cases (1.99%). The mentioned study confirmed the presence of C. abortus in La Pampa Province, not far from Mendoza Province, the location of the present case.…”

Section: Discussionmentioning

confidence: 88%

First report of caprine abortions due to Chlamydia abortus in Argentina

Paolo

Pinedo

Origlia

et al. 2019

Veterinary Medicine & Sci

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Infectious abortions of goats in Argentina are mainly associated with brucellosis and toxoplasmosis. In this paper, we describe an abortion outbreak in goats caused by Chlamydia abortus . Seventy out of 400 goats aborted. Placental smears stained with modified Ziehl‐Neelsen stain showed many chlamydia‐like bodies within trophoblasts. One stillborn fetus was necropsied and the placenta was examined. No gross lesions were seen in the fetus, but the inter‐cotyledonary areas of the placenta were thickened and covered by fibrino‐suppurative exudate. The most consistent microscopic finding was found in the placenta and consisted of fibrinoid necrotic vasculitis, with mixed inflammatory infiltration in the tunica media. Immunohistochemistry of the placenta was positive for Chlamydia spp. The results of polymerase chain reaction targeting 23S rRNA gene performed on placenta were positive for Chlamydia spp. An analysis of 417 amplified nucleotide sequences revealed 99% identity to those of C. abortus pm225 (GenBank AJ 005617) and pm112 (GenBank AJ 005613) isolates. To the best of our knowledge, this is the first report of abortion associated with C. abortus in Argentina.

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Section: Discussionmentioning

confidence: 88%

First report of caprine abortions due to Chlamydia abortus in Argentina

Paolo

Pinedo

Origlia

et al. 2019

Veterinary Medicine & Sci

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“…apresentem baixa especificidade ao hospedeiro, os búfalos e bovinos possuem características semelhantes, fazendo com que o agente também possa estar relacionado a distúrbios reprodutivos na espécie bubalina [15]. m bovinos baixas prevalências foram relatadas na Costa Rica com 0,10% [33], no Iraque com 0,82% [18], no Brasil com 1,42% [36] e na Argentina com 4,78% [31] em comparação às prevalências encontradas em bubalinos que foram de 11,67% [2] e 78,43% [25] no Egito e 57% [15] na Itália. Mesmo não sendo identificados os fatores de risco, o manejo semi-intensivo a extensivo que ocorre em propriedades com búfalos poderia explicar a frequência de anticorpos, já que o contato entre animais criados a pasto pode favorecer a infecção por via oral [26,39].…”

Section: Resultsunclassified

Anti-Chlamydia abortus and Anti-Mycobacterium avium subsp. paratuberculosis Antibodies in Buffaloes in the State of Pernambuco, Brazil

Xavier

Nascimento

Sierra

et al. 2019

Acta Scientiae. Vet.

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Background: The occurrence of economic losses in buffaloes may be related to reproductive problems such as chlamydiosis caused by the bacteria Chlamydia abortus considered as a zoonotic agent; and digestive problems highlighting the infection by Mycobacterium avium subsp. paratuberculosis (Map), responsible for paratuberculosis. There is a little information about these diseases in buffaloes, therefore the aim of this study was to determine the occurrence of anti-Chlamydia abortus and anti- Mycobacterium avium subsp. paratuberculosis (Map) antibodies in water buffaloes in the state of Pernambuco, Brazil.Materials, Methods & Results: The 262 bubaline sera belonging to the serum bank of the Infectious Diseases Laboratory (LIDIC) of the Federal Rural University of Pernambuco (UFRPE) were analyzed. The samples were from nine properties distributed in the municipalities of Agreste and Zona da Mata of the state of Pernambuco. For the detection of anti-Chlamydia abortus and anti-Map antibodies was used a technique of Enzyme immunoabsorption Assay (ELISA) of the IDEXX® by following the manufacturer's instructions. Regarding the detection of anti-Chlamydia abortus antibodies, it was observed that 47.70% (125/262) of the samples were positive. All properties showed at least one positive animal for the investigation of anti-Chlamydia abortus antibodies. It was also verified the occurrence of 7.25% of suspected animals for the investigation of anti-C. abortus antibodies. No positives animals were observed for Map in the bubaline analyzed.Discussion: The occurrence of anti-C. abortus antibodies in buffaloes in the region can be explained by the fact that properties with buffalo breeding has sanitary management that allows the contact between animals, thus increasing the risk of transmission of the agent. In addition, the variation found from 35% to 68.75% may be associated with divergences to the type of management and breeding system used in each property. The percentage of suspected animals may suggest that the number of positive animals is higher or that there were non-specific reactions with other species of Chlamydia, but it is not possible to determine the seroconversion without the accomplishment of paired serology. Because it is a zoonosis, C. abortus may be a risk to the health of the population involved, since the transmission of the bacteria to humans can occur by contact with secretions and excretions of these animals. No positive animals were found for the occurrence of anti-Map antibodies, however, there are reports of the infection identifying animals with clinical signs or properties with a history of the disease, and this may be related to the differences in each property in relation to the history and sanitary management. Moreover, the ELISA test may not be sensitive to the agent depending on the phase of the infection, because if the response is predominantly cellular, the number of antibodies is diminished, making the serological diagnosis difficult. The use of more sensitive tests for the bubaline species can also favor the diagnosis of the infection. Although the occurrence of anti-Map antibodies in the analyzed samples was not detected, it is important to carry out routine epidemiological studies, since the disease has already been registered in water buffaloes in the state of Pernambuco. Considering the occurrence of anti-Chlamydia abortus antibodies it is suggested to conduct studies in order to isolate the agent and verify its real importance in the reproductive disorders of the bubaline species, since this agent causes reproductive losses and has a zoonotic character.

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“…This study highlights the need to advance in the knowledge of C. abortus and its involvement in reproductive problems. Considering the increase in the number of reports about the participation of this bacterium in bovine abortion syndrome, it is worth noting that 50% of the cases ended without a definitive diagnosis ( Kauffold et al., 2007 ; Rojas et al., 2018 ). Additionally, this is a serious public health problem due to the participation of C. abortus in women abortion ( Essig and Longbottom, 2015 ; Longbottom and Coulter, 2003 ; Pospischil et al., 2002 ).…”

Section: Discussionmentioning

confidence: 99%