Detecting and quantifying mites in domestic dust: A novel application for real-time PCR

Roussel, Sandrine; Reboux, Gabriel; Naegele, A.; Martínez, Javier Jesús González; Vacheyrou, Mallory; Scherer, Émeline; Millon, Laurence

doi:10.1016/j.envint.2013.02.002

Cited by 13 publications

(7 citation statements)

References 22 publications

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“…For example, the Dermatophagoïdes farinae target, created in our laboratory (Roussel et al, 2013) could also be used. New EDC will be made and distributed to families to sample dwellings again when children are 3 1/2 years old and will enable us to monitor the children over time.…”

Section: Discussionmentioning

confidence: 99%

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Microbiological characterization of 3193 French dwellings of Elfe cohort children

Rocchi

Reboux

Frossard

et al. 2015

Science of The Total Environment

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Section: Discussionmentioning

confidence: 99%

“…(Rintala and Nevalainen, 2006); -1 house dust mite: Dermatophagoïdes pteronyssinus using primers and probes developed in our laboratory (Roussel et al, 2013). Negative and positive controls were added in each qPCR reaction.…”

Section: Sample Analysismentioning

confidence: 99%

Microbiological characterization of 3193 French dwellings of Elfe cohort children

Rocchi

Reboux

Frossard

et al. 2015

Science of The Total Environment

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“…Consequently, it is important to use diverse sampling methods (air impaction, collection of particles by cyclonic effect, or dust sampling by EDC) at various moments and various distances, and to use several analysis techniques to obtain the best representation of both the quantitative and qualitative bioaerosol composition (Karvonen et al., ). Culture methods with various media and incubation at different temperatures, along with specific molecular tools (qPCR) for microorganisms (Scherer et al., ), and HDM and storage mite quantification (Roussel et al., ) are all needed to assess the concentration variation over an extended time period.…”

Section: Discussionmentioning

confidence: 99%

“…Rapid DNA extraction and PCRs were performed as previously described (Scherer et al, 2014). Specific DNA molds were quantified by qPCR using primers and Taqman TM probes previously described for molds (Haugland et al, 2004), mites (Roussel et al, 2013), and enterobacteria (Serratia sp., Enterobacter sp., and Klebsiella sp. ; Sen and Asher, 2001).…”

Section: Microbiological Analysis and Identificationmentioning

confidence: 99%

Microbiological consequences of indoor composting

et al. 2015

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Recycling of organic waste appeals to more and more people. The aim of this study was to evaluate the microbiological contamination around organic waste bins at three distances over a 12-month period. Contamination near the customary trash of control households was evaluated at the beginning to ensure that there is no recruitment bias. Air samples using the MAS 100 impactor were carried out in 38 dwellings that do household waste composting and in 10 dwellings of controls. Collection of particles by CIP 10 rotating cup sampler and dust samples collected by electrostatic dust collector cloths were acquired in dwellings that do household waste composting. Samples were analyzed by culture and by real-time quantitative PCR. Information about dwelling characteristics and inhabitant practices was obtained by a standardized questionnaire. The genera most often isolated were Penicillium, Aspergillus, Cladosporium and Streptomyces. Near the organic waste bins, bioaerosol samples showed an increase of Acarus siro (P = 0.001). Sedimented dust analyses highlighted an increase of A. siro, Wallemia sebi, Aspergillus versicolor, and Cladosporium sphaerospermum concentrations after a 12-month survey compared to the beginning. Composting favors microorganism development over time, but does not seem to have an effect on the bioaerosol levels and the surface microbiota beyond 0.5 m from the waste bin.

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“…Furthermore, qPCR could be developed for multiplex detection, detecting simultaneously several different species, reducing once more the time and amount of sample needed for an analysis. Many qPCR assays are based on hydrolysis probes, such as the TaqMan ® ones, which are highly specific and useful in multiplex analysis [2, 16, 17]. However, although multiplexing is possible and already successfully performed, the number of targets is still limited to 4 or 5, especially because the number of available fluorophores and quenchers [18], which can be detected at the same time, is limited.…”

Section: Introductionmentioning

confidence: 99%

Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination

et al. 2017

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Considered as a public health problem, indoor fungal contamination is generally monitored using classical protocols based on culturing. However, this culture dependency could influence the representativeness of the fungal population detected in an analyzed sample as this includes the dead and uncultivable fraction. Moreover, culture-based protocols are often time-consuming. In this context, molecular tools are a powerful alternative, especially those allowing multiplexing. In this study a Luminex xMAP® assay was developed for the simultaneous detection of 10 fungal species which are most frequently in indoor air and that may cause health problems. This xMAP® assay was found to be sensitive, i.e. its limit of detection is ranging between 0.05 and 0.01 ng of gDNA. The assay was subsequently tested with environmental air samples which were also analyzed with a classical protocol. All the species identified with the classical method were also detected with the xMAP® assay, however in a shorter time frame. These results demonstrate that the Luminex xMAP® fungal assay developed in this study could contribute to the improvement of public health and specifically to the indoor fungal contamination treatment.

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Detecting and quantifying mites in domestic dust: A novel application for real-time PCR

Cited by 13 publications

References 22 publications

Microbiological characterization of 3193 French dwellings of Elfe cohort children

Microbiological characterization of 3193 French dwellings of Elfe cohort children

Microbiological consequences of indoor composting

Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination

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