Detecting bacteria contamination on medical device surfaces using an integrated fiber-optic mid-infrared spectroscopy sensing method

Mohabatkar, Hassan; Gonzalez, Elizabeth A.; Hitchins, Victoria M.; Ilev, Ilko K.

doi:10.1016/j.snb.2016.03.044

Cited by 19 publications

(12 citation statements)

References 34 publications

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“…Samples need to be dried to avoid this background noise from water bands. While some evidence points towards the FTIR detection of pathogen contamination on medical equipment using hollow-core fibers, this kind of miniaturized FTIR system is only possible due to the substrate being inherently free from the impact of overwhelming biological IR background [37]. Still, advancements in FTIR instrumentation and sample preparation methodology hold this spectroscopic technique as an active area of research for microbe detection and identification.…”

Section: Infrared Spectroscopymentioning

confidence: 99%

Advances in Optical Detection of Human-Associated Pathogenic Bacteria

2020

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Bacterial infection is a global burden that results in numerous hospital visits and deaths annually. The rise of multi-drug resistant bacteria has dramatically increased this burden. Therefore, there is a clinical need to detect and identify bacteria rapidly and accurately in their native state or a culture-free environment. Current diagnostic techniques lack speed and effectiveness in detecting bacteria that are culture-negative, as well as options for in vivo detection. The optical detection of bacteria offers the potential to overcome these obstacles by providing various platforms that can detect bacteria rapidly, with minimum sample preparation, and, in some cases, culture-free directly from patient fluids or even in vivo. These modalities include infrared, Raman, and fluorescence spectroscopy, along with optical coherence tomography, interference, polarization, and laser speckle. However, these techniques are not without their own set of limitations. This review summarizes the strengths and weaknesses of utilizing each of these optical tools for rapid bacteria detection and identification.

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Section: Infrared Spectroscopymentioning

confidence: 99%

Advances in Optical Detection of Human-Associated Pathogenic Bacteria

2020

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“…Detecting several contaminants with a single device has been demonstrated. The use of fiber optic Fourier Transform Infra-Red spectroscopy (Hassan, 2016), quartz crystal sensors (Chang, 2006), and electrochemistry (Safavieh, 2014) has been reported.…”

Section: Introductionmentioning

confidence: 99%

Optical Spectroscopy for the Quality Control of ATMP Fabrication: A New Method to Monitor Cell Expansion and to Detect Contaminations

Wacogne

Legrand

Pieralli

et al. 2020

Proceedings of the 13th International Joint Conference on Biomedical Engineering Systems and Technologies

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Fabrication of Advanced Therapy Medicinal Products takes currently place in clean and sterile environment and is highly sensitive to any contamination sources. It lasts for several days and is extremely expensive. Quality controls must be performed throughout the process, especially to monitor cell growth during the expansion phase and to detect any contaminations. Regular sampling of the bioreactor's content is required and subsequent biological investigation are conducted. Major drawbacks are first, a delayed result of the quality control and second, an added risk to induce new contaminations when sampling the content of the bioreactor. Here, we present a real time optical spectroscopy method which can be used in a closed system manner, hence reducing the risk of sampling-related contamination. Analysing the shape of the absorption spectrum of the bioreactor content allows monitoring the cell growth and alerting users in case of contamination. Cell concentrations are measured with an accuracy of ± 5% and contamination can be detected about 3 hours after it occurred. This allows stopping the fabrication as soon as a problem arises leading to several tens of thousand dollars savings. Consequently, the price of these products should be greatly reduced and they may be proposed to more patients.

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“…Due to the advantages of compact size, immunity to electromagnetic interference and remote sensing capabilities (Zhao et al, 2016), optical fiber sensors have attracted wide research interests in many areas such as automotive, chemical industry, aircraft, medical diagnosis, etc. (Hassan et al, 2016). Numerous optical fiber-based sensor structures have been developed as biosensors, such as fiber Bragg gratings (FBGs) , tapered fibers (Mustapa et al, 2018), fiber interferometers (Sepúlveda et al, 2006; Wang and Wang 2018), photonics crystal fibers (Betancur-Ochoa et al, 2017), dual core fibers (Wysokiński et al, 2018), etc.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive biosensor based on magnetic microspheres enhanced microfiber interferometer

Kumar

Leng

Liu

et al. 2019

Biosensors and Bioelectronics

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A critical barrier for the successful development of fiber sensors for biochemical processes is their limitedly improved sensitivity, restricted by the sensor structural design. To solve this, in this paper, a novel concept was proposed using functionalised modified magnetic microspheres (MMSs) to "amplify" the effect of target biochemical analytes to significantly improve the fiber sensor's sensitivity, which has been demonstrated using human chorionic gonadotropin (hCG) as an example. Two types of antibody hCG, (β and α, both can specifically bind with hCG), were adhered on the surface of fibre sensor and MMSs respectively. Both hCG and MMSs will be specifically captured by the fibre sensor, where MMSs act as an "amplifier" to improve the sensor sensitivity. Experimentally immunomagnetic detection limit of 0.0001 mIU/mL has been achieved, which is the highest reported so far. This newly developed methodology opens a new direction for sensitivity improvement and could be further explored to applications require ultrahigh sensitivity detections such as earlier medical diagnostics.

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Detecting bacteria contamination on medical device surfaces using an integrated fiber-optic mid-infrared spectroscopy sensing method

Cited by 19 publications

References 34 publications

Advances in Optical Detection of Human-Associated Pathogenic Bacteria

Advances in Optical Detection of Human-Associated Pathogenic Bacteria

Optical Spectroscopy for the Quality Control of ATMP Fabrication: A New Method to Monitor Cell Expansion and to Detect Contaminations

Ultrasensitive biosensor based on magnetic microspheres enhanced microfiber interferometer

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