2020
DOI: 10.1101/2020.03.10.986208
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Detecting chromatin interactions along and between sister chromatids with SisterC

Abstract: Accurate chromosome segregation requires chromosome compaction with concordant disentanglement of the two sister chromatids. This process has been studied extensively by microscopy but has remained a challenge for genomic methods, such as Hi-C, because sister chromatids have identical DNA sequences. Here we describe SisterC, a chromosome conformation capture assay that can distinguish interactions between and within sister chromatids. The assay is based on BrdU incorporation during Sphase, which labels the new… Show more

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Cited by 11 publications
(14 citation statements)
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“…The ability of conventional Hi-C to capture intra-chromosomal ( cis ) contacts is well established. However, its ability to reveal trans contacts between sister-chromatids remains limited given the difficulty to distinguish both homologous molecules without incorporation of modified bases ( 51 ). Since Topo IV is mainly known for its involvement in the removal of inter-chromosomal links (or catenanes), presumably between allelic (or near-allelic) loci, we sought to test whether the emergence of prominent Hi-C signals spanning throughout the parE ts contact map could result from trans contacts.…”
Section: Resultsmentioning
confidence: 99%
“…The ability of conventional Hi-C to capture intra-chromosomal ( cis ) contacts is well established. However, its ability to reveal trans contacts between sister-chromatids remains limited given the difficulty to distinguish both homologous molecules without incorporation of modified bases ( 51 ). Since Topo IV is mainly known for its involvement in the removal of inter-chromosomal links (or catenanes), presumably between allelic (or near-allelic) loci, we sought to test whether the emergence of prominent Hi-C signals spanning throughout the parE ts contact map could result from trans contacts.…”
Section: Resultsmentioning
confidence: 99%
“…New methods of chromatin conformation capture constantly emerge, including novel single-cell techniques, such as scSPRITE [ 35 ]. Methods that distinguish contacts of sister chromatids for a population of cells, such as SisterC [ 34 ] and scsHi-C [ 36 ], are of particular interest. We believe that with the development of single-cell Hi-C versions of these methods, DPDchrom would be effectively used to reconstruct conformations of diploid nuclei and genomes containing several copies of chromosomes.…”
Section: Discussionmentioning
confidence: 99%
“…Thus, Hi-SC2 is perfectly suited to analyze the frequency of contacts between sister DNA copies emerging from a replication fork. As a comparison, 3C-derived techniques cannot be used to map interactions between positions separated by less than 1,500 bp (Lajoie et al, 2015;Mitter et al, 2020;Oomen et al, 2020).…”
Section: Discussionmentioning
confidence: 99%
“…However, the resolution limit of microscopy and the restricted number of genomic positions that can be simultaneously visualized hampered the analysis of the many different processes that can influence the cohesiveness of newly replicated DNA molecules. Two conformation capture (3C)-based techniques were recently developed to map sisterchromatid interactions over a whole genome (Mitter et al, 2020;Oomen et al, 2020). The 3C methodology consists in fixing the intracellular conformation adopted by the chromosomes with a crosslinking agent before fragmentation with a restriction enzyme.…”
Section: Introductionmentioning
confidence: 99%