Detecting local adaptation in widespread grassland species – the importance of scale and local plant community

Abstract

Summary1 Adaptation of plant populations to local environments has been shown in many species but local adaptation is not always apparent and spatial scales of differentiation are not well known. In a reciprocal transplant experiment we tested whether: (i) three widespread grassland species are locally adapted at a European scale; (ii) detection of local adaptation depends on competition with the local plant community; and (iii) local differentiation between neighbouring populations from contrasting habitats can be stronger than differentiation at a European scale. 2 Seeds of Holcus lanatus, Lotus corniculatus and Plantago lanceolata from a Swiss, Czech and UK population were sown in a reciprocal transplant experiment at fields that exhibit environmental conditions similar to the source sites. Seedling emergence, survival, growth and reproduction were recorded for two consecutive years. 3 The effect of competition was tested by comparing individuals in weeded monocultures with plants sown together with species from the local grassland community. To compare large-scale vs. small-scale differentiation, a neighbouring population from a contrasting habitat (wet-dry contrast) was compared with the 'home' and 'foreign' populations. 4 In P. lanceolata and H. lanatus, a significant home-site advantage was detected in fitnessrelated traits, thus indicating local adaptation. In L. corniculatus, an overall superiority of one provenance was found. 5 The detection of local adaptation depended on competition with the local plant community. In the absence of competition the home-site advantage was underestimated in P. lanceolata and overestimated in H. lanatus. 6 A significant population differentiation between contrasting local habitats was found. In some traits, this small-scale was greater than large-scale differentiation between countries. 7 Our results indicate that local adaptation in real plant communities cannot necessarily be predicted from plants grown in weeded monocultures and that tests on the relationship between fitness and geographical distance have to account for habitat-dependent small-scale differentiation. Considering the strong small-scale differentiation, a local provenance from a different habitat may not be the best choice in ecological restoration if distant populations from a more similar habitat are available.

Seed source has variable effects on species, communities, and ecosystem properties in grassland restorations

“…v www.esajournals.org for different species are not unique (Bischoff et al 2006, Leimu and Fischer 2008, Hereford 2009, Weißhuhn et al 2011, suggesting that the assumption of local advantage is not well supported by empirical data. One potential reason is that local environments can experience high variability in environmental conditions, which may alter the advantages of different ecotypes.…”

Small-scale diversity of plant communities and distribution of species niches on a copper rock outcrop in Upper Katanga, D.R.Congo

“…Fine-scaled ecological heterogeneity conditions probably play important roles in niche distribution. Some situations might cause divergent selection in the population and thus contribute to ecotypic differentiation (Antonovics et al 1987, Bischoff et al 2006.…”

Field-Scale Transplantation Experiment To Investigate Structures of Soil Bacterial Communities at Pioneering Sites

“…Reciprocal transplantation experiments have been commonly applied in geobotany to study the local adaptation of plants to their habitat (50,52), the effects of the adaptation capacity of plant pathogens and invasive species, or plant performance along environmental gradients (5,33,34). The advantage of these experiments lies in the elimination of biases related to the growth of seedlings in the laboratory and in avoiding an erroneous simulation of natural conditions.…”