Detecting minimal traces of DNA using DNA covalently attached to superparamagnetic nanoparticles and direct PCR-ELISA

Fuentes, Manuel; Mateo, César; Rodríguez, Ana; Casqueiro, Mercedes; Tercero, Juan Carlos; Riese, Hans H.; Fernández‐Lafuente, Roberto; Guisán, José M.

doi:10.1016/j.bios.2005.07.017

Cited by 65 publications

(48 citation statements)

References 39 publications

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“…Many PCR-ELISAs have been developed for the detection, identification, and quantification of a large variety of viruses (2,8,14,23,35,46,47,50,57,62,63,71,75,77,83). In the present study, we have developed and evaluated a PCR-ELISA protocol for the detection and identification of clinically relevant human group A rotavirus G (G1 to G6, G8 to G10, and G12) and P (P [4], P [6], P [8], P [9], and P [14]) genotypes.…”

Section: Discussionmentioning

confidence: 99%

Development of a Microtiter Plate Hybridization-Based PCR-Enzyme-Linked Immunosorbent Assay for Identification of Clinically Relevant Human Group A Rotavirus G and P Genotypes

et al. 2008

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A microtiter plate hybridization-based PCR-enzyme-linked immunosorbent assay (PCR-ELISA) has been used for the detection and identification of a variety of microorganisms. Here, we report the development of a PCR-ELISA for the identification of clinically relevant human rotavirus VP7 (G1 to G6, G8 to G10, and G12) and VP4 (P[4], P[6], P[8], P[9], and P[14]) genotypes. The G and P types of reference human and animal rotavirus strains for which specific probes were available were correctly identified by the PCR-ELISA. In addition, reference strains bearing G or P genotypes for which specific probes were unavailable, such as G11, G14, P[3], P[10], and P[11], did not display any cross-reactivity to the probes. The usefulness of the assay was further evaluated by analyzing a total of 396 rotavirus-positive stool samples collected in four countries: Brazil, Ghana, Japan, and the United States. The results of this study showed that the PCR-ELISA was sensitive and easy to perform without the use of any expensive and sophisticated equipment, the reagents used are easy to obtain commercially and advantageous over multiplex PCR since more than one type-specific probe is used and the selection of probes is more flexible.

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Section: Discussionmentioning

confidence: 99%

Development of a Microtiter Plate Hybridization-Based PCR-Enzyme-Linked Immunosorbent Assay for Identification of Clinically Relevant Human Group A Rotavirus G and P Genotypes

et al. 2008

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“…The application of gold nano-particles for detection of nucleic acid targets on a biosensor platform has been demonstrated to have a sensitivity of 3 Â 10 6 target molecules when evaluated with dilutions of PCR products (Storhoff et al 2004). A study combining biosensor-based nucleic acid detection, with nucleic acid concentration using DNA probes bound to supramagnetic nanoparticles prior to detection on the biosensor achieved detection of 2 molecules of HCV cDNA target in a 2.5 million-fold excess of non-target DNA (Fuentes et al 2006). …”

Section: Signal Amplificationmentioning

confidence: 99%

Nucleic Acid Diagnostic Biosensors

Glynn

O’Connor²

2009

Recognition Receptors in Biosensors

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On the basis of the numbers of scientific articles published using different approaches, use of biosensors is the fastest growing technology for pathogen detection. Biosensor use still lags behind the established technologies such as polymerase chain reaction (PCR) and conventional microbiology but has already surpassed the techniques including gel electrophoresis and ELISA based immunoassays. This chapter provides an overview of nucleic acid diagnostics technologies for pathogen detection with a special emphasis on biosensor technologies including optical, piezoelectric, and electrochemical detection formats.

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“…Alginic acid carrying chelating OH and carboxyl groups provided even stronger bonds. Aldehyde groups were introduced into dextran-coated iron oxide nanoparticles to immobilize and detect DNA [137]. Strong binding of PVA was achieved by crosslinking PVA with glutaraldehyde [82] or by coating iron oxide nanoparticles with PVA functionalized with carboxyl, thiol [138], or phosphate groups [139].…”

Section: Surface Modification Of Magnetic Nanoparticlesmentioning

confidence: 99%

Preparation and properties of magnetic nano‐ and microsized particles for biological and environmental separations

Horák¹,

Babič²,

Macková³

et al. 2007

J of Separation Science

321

177

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The paper presents a critical overview on magnetic nanoparticles and microspheres used as separation media in different fields of chemistry, biochemistry, biology, and environment protection. The preparation of most widely used magnetic iron oxides in appropriate form, their coating or encapsulation in polymer microspheres, and functionalization is discussed in the first part. In the second part, new developments in the main application areas of magnetic composite particles for separation and catalytical purposes are briefly described. They cover separations and isolations of toxic inorganic and organic ions, proteins, and other biopolymers, cells, and microorganisms. Only selected number of relevant papers could be included due to the restricted extent of the review.

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Detecting minimal traces of DNA using DNA covalently attached to superparamagnetic nanoparticles and direct PCR-ELISA

Cited by 65 publications

References 39 publications

Development of a Microtiter Plate Hybridization-Based PCR-Enzyme-Linked Immunosorbent Assay for Identification of Clinically Relevant Human Group A Rotavirus G and P Genotypes

Development of a Microtiter Plate Hybridization-Based PCR-Enzyme-Linked Immunosorbent Assay for Identification of Clinically Relevant Human Group A Rotavirus G and P Genotypes

Nucleic Acid Diagnostic Biosensors

Preparation and properties of magnetic nano‐ and microsized particles for biological and environmental separations

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