Detecting multiple DNA human profile from a mosquito blood meal

Rabêlo, K C N; Albuquerque, Cleide Maria Ribeiro de; Tavares, V. B; Santos, Silvana Maria Elói; Souza, Carla A.; Oliveira, Tatiana Costa; Moura, Ronald; Brandão, Lucas André Cavalcanti; Crovella, Sérgio

doi:10.4238/gmr.15037547

Cited by 8 publications

(9 citation statements)

References 22 publications

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“…DNA fingerprinting represents a useful technique to determine whether there is a single or mixed blood source of fed mosquitoes (e.g. Michael et al ., 2001; Cornet et al ., 2013; Rabêlo et al ., 2016), wherein potential hosts are genetically characterized. However, this technique alone is not sufficiently informative for other key questions such as whether unsuccessful feeding attempts and mixed blood sources are caused by host defences.…”

Section: Determinants Of Host Utilization By Mosquitoesmentioning

confidence: 99%

Understanding host utilization by mosquitoes: determinants, challenges and future directions

et al. 2021

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Mosquito host utilization is a key factor in the transmission of vector‐borne pathogens given that it greatly influences host–vector contact rates. Blood‐feeding patterns of mosquitoes are not random, as some mosquitoes feed on particular species and/or individuals more than expected by chance. Mosquitoes use a number of cues including visual, olfactory, acoustic, and thermal stimuli emitted by vertebrate hosts to locate and identify their blood meal sources. Thus, differences in the quality/intensity of the released cues may drive host selection by mosquitoes at both inter‐ and intra‐specific levels. Such patterns of host selection by mosquitoes in space and time can be structured by factors related to mosquitoes (e.g. innate host preference, behavioural plasticity), to hosts (e.g. emission of host‐seeking cues, host availability) or to both (e.g. pathogen infection). In this study, we review current evidence, from phenomena to mechanisms, of how these factors influence host utilization by mosquitoes. We also review the methodologies commonly used in this research field and identify the major challenges for future studies. To bridge the knowledge gaps, we propose improvements to strengthen traditional approaches and the use of a functional trait‐based approach to infer mosquito host utilization in natural communities.

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Section: Determinants Of Host Utilization By Mosquitoesmentioning

confidence: 99%

Understanding host utilization by mosquitoes: determinants, challenges and future directions

et al. 2021

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“…Further research will show whether the approach is similarly successful with other smaller species of parasitic nematode and other phyla of parasites more generally. Genotyping of hosts from hematophagous arthropods is likely to remain challenging because of the low quality and quantity of nuclear DNA extracted from blood meals as well as the presence of DNA mixtures derived from multiple hosts [ 41 ]. Our samples were collected and stored rapidly after the death of the host.…”

Section: Discussionmentioning

confidence: 99%

Microsatellite profiling of hosts from parasite-extracted DNA illustrated with raccoons (Procyon lotor) and their Baylisascaris procyonis roundworms

2023

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Background Important information on movement pathways and introduction routes of invasive parasites can be obtained by comparing the genetic makeup of an invader with its spatial genetic structure in other distribution areas. Sometimes, the population genetic structure of the host might be more informative than that of the parasite itself, and it is important to collect tissue samples of both host and parasite. However, host tissue samples are frequently not available for analysis. We aimed to test whether it is possible to generate reliable microsatellite profiles of host individuals by amplifying DNA extracted from a nematode parasite, using the raccoon (Procyon lotor) and the raccoon roundworm (Baylisascaris procyonis) as a test case. Methods Between 2020 and 2021, we collected tissue as well as a single roundworm each from 12 raccoons from central Germany. Both the raccoon and the roundworm DNA extracts were genotyped using 17 raccoon-specific microsatellite loci. For each roundworm DNA extract, we performed at least eight amplification reactions per microsatellite locus. Results We extracted amplifiable raccoon DNA from all 12 roundworms. We obtained at least two amplification products for 186 of the 204 possible genotypes. Altogether 1077 of the 1106 genotypes (97.4%) matched the host-DNA derived reference genotypes and thus did not contain genotyping errors. Nine of the 12 roundworm-derived genetic profiles matched the reference profiles from the raccoon hosts, with one additional genetic profile containing genotyping errors at a single locus. The remaining two genetic profiles were deemed unsuitable for downstream analysis because of genotyping errors and/or a high proportion of missing data. Conclusions We showed that reliable microsatellite-based genetic profiles of host individuals can be obtained by amplifying DNA extracted from a parasitic nematode. Specifically, the approach can be applied to reconstruct invasion pathways of roundworms when samples of the raccoon hosts are lacking. Further research should assess whether this method can be replicated in smaller species of parasitic nematodes and other phyla of parasites more generally. Graphical Abstract

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“…Recently, we have successfully obtained a full human STR profile from mosquito blood meal up to 36 h post-feeding (Ahmed et al 2023). Many other studies have successfully retrieved vertebrate host DNAs from mosquito blood meals (Spitaleri et al 2006, Rabêlo et al 2015, 2016) and even from small and biologically contaminated blood traces (Reeves et al 2016). These studies, and many others, have successfully obtained forensically valuable information from a mosquito blood meal.…”

Section: Discussionmentioning

confidence: 99%

Forensic analysis of mosquito blood meal digestion process and the impact of heroin opiate: determination of the post-feeding interval as a PMI estimation

Alharbi,

Elnakady,

Aldahmash

et al. 2023

Journal of Medical Entomology

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Females of some mosquito species are anthropophilic, as they feed on human blood to support egg production and, hence, are forensically valuable if found at a crime scene. The present study investigated the blood meal digestion process in Culex pipiens L. both with and without heroin and proposed a method for estimating the post-feeding interval (PFI). Mosquitoes were fed on a control mouse, a heroin-injected mouse, or in vitro heroin-treated mouse blood. The blood meal digestion was then investigated at different hours post-feeding. Data showed that the blood meal size ingested by control mosquitoes was 0.681 ± 0.04 mg/mosquito and was completely digested within 45 h post-feeding. An estimation of the PFI was proposed in terms of the rate of hemoglobin (Hb) digestion. The blood meal size of the mosquitoes fed on the in vitro heroin-treated blood and the heroin-injected mouse was 0.96 ± 0.06 and 0.79 ± 0.01 mg/mosquito and was completely digested within 50 and 55 h post-feeding, respectively. The digestion of Hb started similarly in all experimental mosquitoes until 10 h post-feeding, after which it significantly decreased in heroin-treated blood meals compared with the control ones. This may suggest that heroin impacted the digestion process, as it took an extra 5–10 h to complete. These findings could be valuable in the forensic context since an estimation of PFI is proposed as a potential estimation of the postmortem interval (PMI). However, care should be taken as heroin in the host blood has significantly impacted the overall digestion process and, hence, may bias the PFI/PMI estimation.

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Detecting multiple DNA human profile from a mosquito blood meal

Cited by 8 publications

References 22 publications

Understanding host utilization by mosquitoes: determinants, challenges and future directions

Understanding host utilization by mosquitoes: determinants, challenges and future directions

Microsatellite profiling of hosts from parasite-extracted DNA illustrated with raccoons (Procyon lotor) and their Baylisascaris procyonis roundworms

Forensic analysis of mosquito blood meal digestion process and the impact of heroin opiate: determination of the post-feeding interval as a PMI estimation

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