Detecting of biofilm formation in the clinical isolates of Pseudomonas
                        aeruginosa and Escherichia coli: an evaluation of different screening
                        methods

Mohsenzadeh, Amin; Fazel, Alireza; Bavari, Shirin; Borji, Sanaz; Pourasghar, Sahar; Azimi, Taher; Sabati, Hoda

doi:10.52547/jcbior.2.2.56

Cited by 10 publications

(5 citation statements)

References 15 publications

(20 reference statements)

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“…3), while non-membrane-forming E. coli isolates were found to be bright pink, red, or winecolored colonies. Mohsenzadeh et al (2021) noted that out of 100 E. coli and P. aeruginosa isolates, 38 (38 %) demonstrated a biofilm-positive phenotype under optimum circumstances in MTPA and isolates were further categorised as strong, moderate, weak, and no biofilm on Congo red agar The present study showed the number of UPEC that formed biofilm in vitro was 71%, which was comparable to the studies that were carried out by previous studies of Subramanian et al…”

Section: Detection Of Biofilm Formation By Congo Red Agar (Cra)supporting

confidence: 89%

Molecular detection of extended spectrum ß-lactamase genes in Escherichia coli isolates from urinary tract infected patients

Hussein

Alwash²

2022

JANS

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Extended-spectrum ß-lactamases (ESBL) are a major source of concern. ESBL have been recorded around the world. Globally, the number of people infected with Enterobacteriaceae that produce extended-spectrum beta-lactamase (ESBL) is on the rise. It has been a rise in resistance to ß-lactam antibiotics among them. In this study, the objective was to collect Escherichia coli isolates from Urinary tract infection patients using selective medium, determine the prevalence of ESBL-producing E. coli, phylogenetic groupings of isolates, ESBL production, and biofilm formation among the isolates of E. coli isolates. The study included 250 E. coli samples from male and female subjects and grown on a selective medium. The isolated bacteria were submitted to different tests, including the detection of biofilm development and testing of the phylogenetic grouping of the E. coli isolate using triplex-PCR analysis. Representatives of each isolate were phenotypically evaluated for antibiotic resistance and classified into phylogenetic groupings. The results of extended-spectrum ß -lactams antibiotics showed the greatest resistance levels. There were 100% resistance rates for Ceftazidime-Clavulantae (CZC) and Cefotaxime-Clavulantae (CTC), 78.7% for Ceftazidime (CAZ), 86.7% for Cefotaxime CTX, 84% for Aztreonam (ATM), 87.3% for Ceftriaxone (CRO) and 83.3% for Cefpodoxime (CPD). E. coli isolates belonging to phylogroup B2 (91, 91%), and subtyping B23 (75, 75%) were the most common among UTI patients. ESBL-producing E. coli isolates were prevalent in individuals with UTIs. Most E. coli isolates from UTI patients at Al-Hillah hospitals belonged to phylogroup B2, followed by D, B1, and A. B2 was the most prevalent group in the study. This study examined the dissemination of ESBL genes in phylogenetic groups of the E. coli isolates from UTIs patients in the Al-Hillah, Iraq.

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Section: Detection Of Biofilm Formation By Congo Red Agar (Cra)supporting

confidence: 89%

Molecular detection of extended spectrum ß-lactamase genes in Escherichia coli isolates from urinary tract infected patients

Hussein

Alwash²

2022

JANS

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“…Biofi lm production is recognized as a contributing factor to the development of resistance in P. aeruginosa. Th e presence of biofi lm serves as a formidable obstacle for both the immune system and antibiotics, thus constituting a primary etiological factor in the development and progression of infectious diseases (Mohsenzadeh et al, 2021). Th e formation of a mucous layer and utilization of host proteins by biofi lm contribute signifi cantly to pathogenicity.…”

Section: Results P Aeruginosa Was Identifi Ed In 83mentioning

confidence: 99%

Характеристика генів інтегронів клінічних ізолятів Pseudomonas aeruginosa, які реалізують резистентність до антибіотиків та біоплівкоутворення цими штамами

Alfarras,

Al-Daraghi

2024

Mikrobiol. Z.

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Pseudomonas aeruginosa is a bacterium that holds significant clinical and epidemiological significance. It serves as the principal etiological cause of opportunistic infections in individuals with impaired immune systems. Integrons are known to have a notable impact on Gram-negative bacteria, particularly in the context of P. aeruginosa, a bacterium recognized for its ability to develop resistance to antimicrobial drugs. Aim. To systematically characterize and detect integron genes (intI, intII, intIII) with antibiotic-resistant and biofilm-forming capabilities in isolated P. aeruginosa. Methods. A total of 209 samples were collected from Al Yarmouk Teaching Hospital in Baghdad City, Iraq to isolate P. aeruginosa. The process of bacterial identification was carried out phenotypically and by biochemical tests. Antibiotic susceptibility was measured using the Vitek2 system. Biofilm quantification was done by the microtiter method. The PCR approach was employed to assess the presence of class 1, 2, and 3 integrons. Results. P. aeruginosa was identified in 83 isolates by using a combination of morphological and biochemical examinations where all isolates showed the ability to grow a selective medium on cetrimide agar for P. aeruginosa. The results also showed significant variances (p < 0.05) among the percentage of a number of samples and isolated P. aeruginosa. The burn and wound infection scored the highest percentages (25% and 19%) based on the positivity of P. aeruginosa, whereas burn and ear sites scored the highest percentage (58% and 50%). Also, the isolates show the ability to form biofilm at a percentage of 68.7% with resistance to a high number of antibiotics. The multidrug-resistant and sensitive P. aeruginosa isolates scored high percentages (49.4% and 34.9%) whereas potentially pan drug-resistant and extensively drug-resistant isolates scored low percentages (2.4% and 13.3%). PCR results showed that integron I scored the highest percentage (100%) compared to integron 2 found in 3 (10%) isolates, and no intI3 gene was detected in any of the P. aeruginosa isolates. Conclusions. Overall, the findings of the present investigation indicate that integrons and biofilm development are recognized as significant factors contributing to antibiotic resistance in P. aeruginosa. The prevalence of class 1 integrons is shown to be significantly high in all bacterial isolates, with a complete occurrence rate of 100%. This high incidence of class 1 integrons is associated with the development of resistance to crucial antibiotics, including β-lactams, aminoglycosides, and cephalosporins.

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“…The TiO 2 /CIP and TiO 2 /CIP/Cs were the most effective samples for preventing biofilm formation, whereas TiO 2 was the least effective, showing no inhibition at the highest tested concentration (64 μg/mL) ( Figure 10 ). Biofilm production strength was classified based on the optical density (OD) of the isolate: ≤0.232 for no biofilm detected, 0.232 < OD isolate ≤ 0.464 for weak biofilm formation, 0.464 < OD isolate ≤ 0.929 for moderate biofilm formation, and OD isolate > 0.929 for strong biofilm formation [ 50 ]. To compensate for background absorbance, the OD reading value (570 nm) of the blank was deducted from the sample values.…”

Section: Resultsmentioning

confidence: 99%

Ciprofloxacin-Loaded Titanium Nanotubes Coated with Chitosan: A Promising Formulation with Sustained Release and Enhanced Antibacterial Properties

et al. 2022

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Due to their high entrapment efficiency, anodized titanium nanotubes (TiO2-NTs) are considered effective reservoirs for loading/releasing strong antibiotics whose systemic administration is associated with diverse and severe side-effects. In this study, TiO2-NTs were synthesized by anodic oxidation of titanium foils, and the effects of electrolyte percentage and viscosity on their dimensions were evaluated. It was found that as the water content increased from 15 to 30%, the wall thickness, length, and inner diameter of the NTs increase from 5.9 to 15.8 nm, 1.56 to 3.21 µm, and 59 to 84 nm, respectively. Ciprofloxacin, a highly potent antibiotic, was loaded into TiO2-NTs with a high encapsulation efficiency of 93%, followed by coating with different chitosan layers to achieve a sustained release profile. The prepared formulations were characterized by various techniques, such as scanning electron microscopy, differential scanning calorimetry, and contact measurement. In vitro release studies showed that the higher the chitosan layer count, the more sustained the release. Evaluation of antimicrobial activity of the formulation against two endodontic species from Peptostreptococcus and Fusobacterium revealed minimum inhibitory concentrations (MICs) of 1 µg/mL for the former and the latter. To summarize, this study demonstrated that TiO2-NTs are promising reservoirs for drug loading, and that the chitosan coating provides not only a sustained release profile, but also a synergistic antibacterial effect.

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Detecting of biofilm formation in the clinical isolates of Pseudomonas aeruginosa and Escherichia coli: an evaluation of different screening methods

Cited by 10 publications

References 15 publications

Molecular detection of extended spectrum ß-lactamase genes in Escherichia coli isolates from urinary tract infected patients

Molecular detection of extended spectrum ß-lactamase genes in Escherichia coli isolates from urinary tract infected patients

Характеристика генів інтегронів клінічних ізолятів Pseudomonas aeruginosa, які реалізують резистентність до антибіотиків та біоплівкоутворення цими штамами

Ciprofloxacin-Loaded Titanium Nanotubes Coated with Chitosan: A Promising Formulation with Sustained Release and Enhanced Antibacterial Properties

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