Detecting prostate cancer by intracellular macrophage prostate‐specific antigen (PSA): a more specific and sensitive marker than conventional serum total PSA

Herwig, Ralf; Mitteregger, Dieter; Djavan, Bob; Kramer, Gero; Margreiter, Markus; Leers, Math P.G.; Glodny, Bernhard; Haider, David; Hörl, W H; Marberger, Michael

doi:10.1111/j.1365-2362.2008.01953.x

Cited by 11 publications

(11 citation statements)

References 33 publications

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“…Other potential blood and urine based biomarkers for prostate cancer include: prostate cancer antigen 3 [PCA3] [ 11 ], intracellular PSA [ 12 ], PSA derivatives [ 13 ], early prostate cancer antigen 2 [ 14 ], annexin A3 [ 15 ], the fusion gene TMPRSS2:ERG [ 16 , 17 ], human kallikrein 2 [ 18 ] and the Engrailed-2 protein (EN2) [ 19 , 20 ]. However, none of these are used for screening [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Urinary Volatile Organic Compounds for the Detection of Prostate Cancer

et al. 2015

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The aim of this work was to investigate volatile organic compounds (VOCs) emanating from urine samples to determine whether they can be used to classify samples into those from prostate cancer and non-cancer groups. Participants were men referred for a trans-rectal ultrasound-guided prostate biopsy because of an elevated prostate specific antigen (PSA) level or abnormal findings on digital rectal examination. Urine samples were collected from patients with prostate cancer (n = 59) and cancer-free controls (n = 43), on the day of their biopsy, prior to their procedure. VOCs from the headspace of basified urine samples were extracted using solid-phase micro-extraction and analysed by gas chromatography/mass spectrometry. Classifiers were developed using Random Forest (RF) and Linear Discriminant Analysis (LDA) classification techniques. PSA alone had an accuracy of 62–64% in these samples. A model based on 4 VOCs, 2,6-dimethyl-7-octen-2-ol, pentanal, 3-octanone, and 2-octanone, was marginally more accurate 63–65%. When combined, PSA level and these four VOCs had mean accuracies of 74% and 65%, using RF and LDA, respectively. With repeated double cross-validation, the mean accuracies fell to 71% and 65%, using RF and LDA, respectively. Results from VOC profiling of urine headspace are encouraging and suggest that there are other metabolomic avenues worth exploring which could help improve the stratification of men at risk of prostate cancer. This study also adds to our knowledge on the profile of compounds found in basified urine, from controls and cancer patients, which is useful information for future studies comparing the urine from patients with other disease states.

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Section: Introductionmentioning

confidence: 99%

Urinary Volatile Organic Compounds for the Detection of Prostate Cancer

et al. 2015

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“…The location of the parasite that causes malaria is found within blood cells and, similarly, the defective proteins produced by various oncogenes and proto-oncogenes that result in cancer or leukaemia are found within the cells. In the case of cancer diagnostics, Herwig demonstrated that the measurement of intracellular prostate specific antigen (PSA) is a better marker for prostate cancer than the traditional serum measurement [2].…”

Section: Introductionmentioning

confidence: 99%

The Dual Role of Paramagnetic Particles for Integrated Lysis and Measurement in a Rapid Immunoassay for Intracellular Proteins

Sharif

Kiely

Wraith

et al. 2013

IEEE Trans. Biomed. Eng.

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A novel, integrated lysis and immunoassay methodology and system for intracellular protein measurement are described. The method uses paramagnetic particles both as a lysis agent and assay label resulting in a rapid test requiring minimal operator intervention, the test being homogeneous and completed in less than 10 min. A design study highlights the critical features of the magnetic detection system used to quantify the paramagnetic particles and a novel frequency-locked loop-based magnetometer is presented. A study of paramagnetic particle enhanced lysis demonstrates that the technique is more than twice as efficient at releasing intracellular protein as ultrasonic lysis alone. Results are presented for measurements of intracellular prostate specific antigen in an LNCAP cell line. This model was selected to demonstrate the rapidity and efficiency of intracellular protein quantification. It was shown that, on average, LNCAP cells contained 0.43 fg of prostate specific antigen. This system promises an attractive solution for applications that require a rapid determination of intracellular proteins.

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“…In breast, gastric, urogenital, glioma, cholangiocarcinoma, Hodgkin lymphoma and glioma high density TAM infiltration is associated with worse overall survival, enhanced tumour growth and metastases. Interestingly though, in CRC high density TAM infiltration is associated with better overall survival 38 . It is suggested that TAM ontogeny, phenotype, their products and activation state, are more informative for patient prognosis than their absolute number in and around tumours 8,39…”

Section: Tumour-associated Macrophages (Tam)mentioning

confidence: 97%

“…This review will not further dwell on the origins, functions and interactions of the other mentioned cells in the stromal border or their role in cancer development in general 40 . Macrophages contribute to a tumour microenvironment suitable for either tumour progression or tumour regression, where the outcome is based on an environmental balance shift either way 11,38 . TAM were thought to predominantly lean towards a M2-like phenotype [43][44][45] , but recent research shows that a dynamic activation switch occurs among TAM as the tumour develops, shifting from M1-state through different sub-states towards M2.…”

Section: Tam In Stromal Tissuementioning

confidence: 99%

Macrophages in colorectal cancer: proof of principle for diagnostic application

Japink¹

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People interested in the research are advised to contact the author for the final version of the publication, or visit the DOI to the publisher's website.• The final author version and the galley proof are versions of the publication after peer review.• The final published version features the final layout of the paper including the volume, issue and page numbers. Link to publication General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal.If the publication is distributed under the terms of Article 25fa of the Dutch Copyright Act, indicated by the "Taverne" license above, please follow below link for the End User Agreement:

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Detecting prostate cancer by intracellular macrophage prostate‐specific antigen (PSA): a more specific and sensitive marker than conventional serum total PSA

Abstract: In this study, we demonstrate that imPSA is a new prostate cancer screening method that is highly sensitive and more specific than standard PSA testing.

Cited by 11 publications

References 33 publications

Urinary Volatile Organic Compounds for the Detection of Prostate Cancer

Urinary Volatile Organic Compounds for the Detection of Prostate Cancer

The Dual Role of Paramagnetic Particles for Integrated Lysis and Measurement in a Rapid Immunoassay for Intracellular Proteins

Macrophages in colorectal cancer: proof of principle for diagnostic application

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