Detecting staphylococcal enterotoxin B using an automated fiber optic biosensor

King, Keeley D.; Anderson, George P.; Bullock, Karen; Regina, Meredith J.; Saaski, E. W.; Ligler, Frances S.

doi:10.1016/s0956-5663(98)00108-0

Cited by 84 publications

(38 citation statements)

References 12 publications

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“…Binding inhibition assay is an alternative test format of competitive assay, with the antigens being immobilized and the antibodies being labeled [39,40]. In the sandwich assay [41][42][43], antibodies to analytes are first immobilized to a sensor. Next, the sensor is incubated with the analytes, and complexes are formed between the analytes and the immobilized antibodies.…”

Section: Antigens/antibodiesmentioning

confidence: 99%

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

et al. 2005

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(ABSTRACT)Fiber-optic Fabry-Perot interferometric (FFPI) sensors have been widely used due to their high sensitivity, ease of fabrication, miniature size, and capability for multiplexing. However, direct measurement of self-assembled thin films, receptor immobilization process or biological reaction is limited in the FFPI technique due to the difficulty of forming Fabry-Perot cavities by the thin film itself. Novel methods are needed to provide an accurate and reliable measurement for monitoring the thin-film growth in the nanometer range and under various conditions. In this work, two types of fiber-optic multicavity Fabry-Perot interferometric (MFPI) sensors with built-in temperature compensation were designed and fabricated for thin-film measurement, with applications in chemical and biological sensing. Both the tubing-based MFPI sensor and microgap MFPI sensor provide simple, yet high performance solutions for thin-film sensing. The temperature dependence of the sensing cavity is compensated by extracting the temperature information from a second multiplexed cavity. This provides the opportunity to examine the thin-film characteristics under different environment temperatures.To demonstrate the potential of this structure for practical applications, immunosensors were fabricated and tested using these structures. Self-assembled polyelectrolytes served as a precursor film for immobilization of antibodies to ensure they retain their biological activity. This not only provides a convenient method for protein immobilization but also presents the possibility of increasing the binding capacity

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Section: Antigens/antibodiesmentioning

confidence: 99%

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

et al. 2005

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“…These methods include polymerase chain reaction (PCR), DNA probes, immunosensors, flow cytometry, and gas chromatography coupled with mass spectrometry (GC-MS) 32,33 . These are the basic tools being employed in the development of more sensitive and robust detection systems like bio detector (BD), rapid analyte measurement platform (RAMP), microbial identification system (MIS), and biohazard detection system (BDS) [16][17][18]34 .…”

Section: Rapid Specific and Sensitive Methodsmentioning

confidence: 99%

Biological Warfare Agents

Kamboj¹,

Goel²,

Singh³

2006

DSJ

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There is a long historic record of use of biological warfare (BW) agents by warring countries against their enemies. However, the frequency of their use has increased since the beginning of the twentieth century. World war I witnessed the use of anthrax agent against human beings and animals by Germans, followed by large-scale field trials by Japanese against war prisoners and Chinese population during world war II. Ironically, research and development in biological warfare agents increased tremendously after the Geneva Protocol, signed in 1925, because of its drawbacks which were overcome by Biological and Toxin Weapons Convention (BTWC) in 1972. Biological warfare programme took back seat after the 1972 convention but biological agents regained their importance after the bioterrorist attacks of anthrax powder in 2001. In the light of these attacks, many of which turned out to be hoax, general awareness is required about biological warfare agents that can be used against them. This review has been written highlighting important biological warfare agents, diseases caused by them, possible therapies and other protection measures.

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“…Use of polystyrene fiber probe can be a robust alternative. 13 Experiments in our lab have shown that strong autofluorescence associated with polystyrene fiber probe significantly reduces signal to background. In this study, we used 300-μm-diam rugged CTFOB probes, fabricated in our lab from a 600-μm silica-silica multimode fiber.…”

Section: Introductionmentioning

confidence: 98%

“…In last two decades, significant progress has been made in the field of evanescence wave-induced fluorescence/chemiluminescencebased fiber-optic biosensors. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15] Despite these advantages, the fiber-optic biosensors have been used, most of the time, only in detection of various kinds of biological analytes. 5,6,8,[11][12][13][14][15][16] Work on quantitative estimation of bioanalytes using fiber-optic sensors is very limited.…”

Section: Introductionmentioning

confidence: 99%

Development of combination tapered fiber-optic biosensor dip probe for quantitative estimation of interleukin-6 in serum samples

et al. 2010

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Abstract.A combination tapered fiber-optic biosensor (CTFOB) dip probe for rapid and cost-effective quantification of proteins in serum samples has been developed. This device relies on diode laser excitation and a charged-coupled device spectrometer and functions on a technique of sandwich immunoassay. As a proof of principle, this technique was applied in a quantitative estimation of interleukin IL-6. The probes detected IL-6 at picomolar levels in serum samples obtained from a patient with lupus, an autoimmune disease, and a patient with lymphoma. The estimated concentration of IL-6 in the lupus sample was 5.9 ± 0.6 pM, and in the lymphoma sample, it was below the detection limit. These concentrations were verified by a procedure involving bead-based xMAP technology. A similar trend in the concentrations was observed. The specificity of the CTFOB dip probes was assessed by analysis with receiver operating characteristics. This analysis suggests that the dip probes can detect 5-pM or higher concentration of IL-6 in these samples with specificities of 100%. The results provide information for guiding further studies in the utilization of these probes to quantify other analytes in body fluids with high specificity and sensitivity. C 2010 Society of Photo-Optical Instrumentation Engineers.

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Detecting staphylococcal enterotoxin B using an automated fiber optic biosensor

Cited by 84 publications

References 12 publications

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

Biological Warfare Agents

Development of combination tapered fiber-optic biosensor dip probe for quantitative estimation of interleukin-6 in serum samples

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