Detecting total toxicity in water using a mediated biosensor system with flow injection

Yong, Daming; Liu, Changyu; Zhu, Chengzhou; Liu, Ling; Zhai, Junfeng; Dong, Shaojun

doi:10.1016/j.chemosphere.2015.05.031

Cited by 11 publications

(4 citation statements)

References 30 publications

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“…As a result the greatest response current was observed at high concentration of ferricyanide. On the other hand, at minimum concentration of 0.25 mM of ferricyanide, the smallest response current was detected, indicated that the catalytic response of E.coli depends on the concentration of ferricyanide which was consistent with a previous report [42].…”

Section: Effects Of Mediator Concentration On Ecoli Catalytic Responsesupporting

confidence: 92%

“…This result indicated that ferricyanide were mediated by the catalytic response of E.coli. The current response was measured at higher (µ A level) even at higher concentration of ferricyanide (45 mM; nA current) reported in other study [42]. This might be due to the high rotation rate of RDE which allows the rapid mass transfer [43] to the electrode surface.…”

Section: Investigation Of Catalytic Response Of Ecolimentioning

confidence: 66%

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Rotating Droplet Hydrodynamic Electrochemistry for Water Toxicity Bioassay Based on an Electron-Transfer Mediator

Sazawa,

Yeasna,

Sugawara

et al. 2024

Preprint

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An electrochemical bioassay based on the rotating droplet electrochemistry by using electron transfer mediator was developed for the evaluation of a wide variety of pollutants such as antibiotics, heavy metals and pesticides in the water environment. Ferricyanide was used as electron transfer mediator for obtaining the catalytic response of Escherichia coli. The electrochemical response of E.coli was measured via hydrodynamic chronoamperometry in a microdroplet on a screen-printed carbon electrode (SPCE). The constructed electrode system successfully evaluate the catalytic response of E.coli solution in presence of ferricyanide. The assay for antibiotic toxicity on E.coli was carried out. The EC50 for ampicillin, sulfamonomethoxine, chlorotetracycline, tetracycline and oxytetracycline valuated by the pre-incubation method were 0.26, 0.77, 5.25, 18.5, 19.0 µM, respectively. The toxicity order was ampicillin &gt; sulfamonomethoxine &gt; chlorotetracycline &gt; tetracycline&gt;oxytetracycline. The proposed method can be used to evaluate the antibiotic toxicities in different real sample, such as pond water, powder and raw milk. Recoveries were found in the range 90 and 99%. The developed methods do not require additional incubation time to evaluate toxicity.

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Section: Effects Of Mediator Concentration On Ecoli Catalytic Responsesupporting

confidence: 92%

Section: Investigation Of Catalytic Response Of Ecolimentioning

confidence: 66%

Rotating Droplet Hydrodynamic Electrochemistry for Water Toxicity Bioassay Based on an Electron-Transfer Mediator

Sazawa,

Yeasna,

Sugawara

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Previous studies have shown that the microbial concentration affects the sensitivity of biotoxicity assessment. 40,41 Fig. S4b † illustrated the influence of microbial concentrations (OD 600 ) on inhibition response caused by Cu 2+ (6 mg L −1 ) on E. coli.…”

Section: Microbial Concentrationmentioning

confidence: 99%

Adaptive use of a personal glucose meter (PGM) for acute biotoxicity assessment based on the glucose consumption of microbes

Fang

Gao

et al. 2016

Analyst

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In this study, a new method for acute biotoxicity assessment was proposed by measuring the glucose consumption of microbes with a personal glucose meter (PGM). To obtain an ideal biotoxicity assessment performance, an appropriate microbe was selected first, and then the relevant parameters, such as temperature and microbial concentration were optimized. Under the optimized parameters, the acute biotoxicity of four environmental pollutants (As(3+), Ni(2+), 4-chlorophenol, and 2,4-dichlorophenol), three wastewater samples and three soil samples were evaluated. This technology breakthrough will help us develop a low cost, easy to use water-environmental early-warning kit.

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“…In the past decades, many researches of biotoxicity assessment have been mainly focused on the sensitivity and pollutant degradation analysis of aquatic organisms and invertebrates [9][10][11][12], toxicity assessment of bioluminescent bacteria in water and atmospheric particulate matter [13,14] and electrochemical detection of microbial respiratory inhibition [15][16][17]. For example, higher concentration of triclosan has produced great oxidative stress to goldfish under the acidic condition [18].…”

Section: Introductionmentioning

confidence: 99%

Acute biotoxicity assessment of heavy metals in sewage sludge based on the glucose consumption ofEscherichia coli

Tao

2019

R. Soc. open sci.

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As a simple and feasible method for acute biotoxicity assessment, personal glucose meter (PGM) can be successfully applied in the early warning of environmental pollutants in sewage. In this paper, the acute biotoxicity of single and joint heavy metals in sewage and real sludge samples was systematically described based on the glucose metabolism of Escherichia coli (E. coli). Results indicated that the biotoxicity order of five single heavy metals in sewage was Hg2+ > As3+ > Cu2+ > Zn2+ > Cd2+. The joint heavy metals of Cu2+ + Zn2+, Cu2+ + Cd2+, and Cu2+ + Hg2+ produced synergistic effects, while Cu2+ + As3+ and Cd2+ + Zn2+ possessed antagonistic effects for the combined biotoxicity. In spiked sludge, Cd2+ and Zn2+ owned higher biotoxicity than Cu2+ and As3+. Notably, the electroplate factory and housing estate sludge respectively showed the highest and lowest inhibition rates as 57.4% and 17.7% under the real sludge biotoxicity assessment. These results demonstrated that PGM was a sensitive and portable method, which could be widely used for acute biotoxicity assessment of heavy metals in sewage sludge.

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Detecting total toxicity in water using a mediated biosensor system with flow injection

Cited by 11 publications

References 30 publications

Rotating Droplet Hydrodynamic Electrochemistry for Water Toxicity Bioassay Based on an Electron-Transfer Mediator

Rotating Droplet Hydrodynamic Electrochemistry for Water Toxicity Bioassay Based on an Electron-Transfer Mediator

Adaptive use of a personal glucose meter (PGM) for acute biotoxicity assessment based on the glucose consumption of microbes

Acute biotoxicity assessment of heavy metals in sewage sludge based on the glucose consumption ofEscherichia coli

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