Detecting trypsin at liquid crystal/aqueous interface by using surface-immobilized bovine serum albumin

Chuang, Cheng‐Hao; Lin, Yi-Cheng; Chen, Wei-Long; Chen, Yu-Hsuan; Chen, Yu-Xun; Chen, Chieh-Ming; Shiu, Hung-Wei; Chang, Lo-Yueh; Chen, Chia-Hao; Chen, Chih-Hsin

doi:10.1016/j.bios.2015.11.049

Cited by 36 publications

(8 citation statements)

References 34 publications

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“…The BSA was cleaved by trypsin and released peptide fragments, which disrupted the orientation of LC and generated a bright optical image. This method obtained a lower LOD at 10 ng/mL compared with the previous monolayer-based method [ 113 ]. Specially, a novel integrated LC-based trypsin sensing device was designed by Ping et al by mixing gelatin hydrogel with surfactant CTAB.…”

Section: Applications In Lc Biochemical Sensorsmentioning

confidence: 94%

State-of-the-Art Development in Liquid Crystal Biochemical Sensors

et al. 2022

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As an emerging stimuli-responsive material, liquid crystal (LC) has attracted great attentions beyond display applications, especially in the area of biochemical sensors. Its high sensitivity and fast response to various biological or chemical analytes make it possible to fabricate a simple, real-time, label-free, and cost-effective LC-based detection platform. Advancements have been achieved in the development of LC-based sensors, both in fundamental research and practical applications. This paper briefly reviews the state-of-the-art research on LC sensors in the biochemical field, from basic properties of LC material to the detection mechanisms of LC sensors that are categorized into LC-solid, LC–aqueous, and LC droplet platforms. In addition, various analytes detected by LCs are presented as a proof of the application value, including metal ions, nucleic acids, proteins, glucose, and some toxic chemical substances. Furthermore, a machine-learning-assisted LC sensing platform is realized to provide a foundation for device intelligence and automatization. It is believed that a portable, convenient, and user-friendly LC-based biochemical sensing device will be achieved in the future.

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Section: Applications In Lc Biochemical Sensorsmentioning

confidence: 94%

State-of-the-Art Development in Liquid Crystal Biochemical Sensors

et al. 2022

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“…Typical LC sensing technology involves the use of a grid filled with LC or the preparation of an LC cell. Grid filling, unfortunately, is unstable at the interface between the LC and the solution to be tested, and it is challenging to use an LC cell for real-time monitoring [22][23][24] . On the other hand, LC droplets have a large specific surface area, can detect target substances more sensitively, can be manufactured by simple methods, and have good potential for gas sensing.…”

Section: Introductionmentioning

confidence: 99%

Liquid-crystal-droplet-based Monitoring System for Water-soluble Inorganic Acidic Gases from the Atmosphere

Jang

2020

BioChip J

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A liquid crystal (LC)-based pH sensor for real-time monitoring of variations in localized pH values near the LC droplets was developed, and its applicability for the detection of acidic gases was explored. It was found that 4-cyano-4′-pentylbiphenyl (5CB), when doped with hexanoic acid (HA), shows a bright-fan-shaped (planar orientation of LCs) to dark-cross (homeotropic orientation of LCs) optical response to a minimal change of the pH value (from 6.5 to 6.6). The fast and intensive pH-driven optical response can be explained mainly with the orientational transitions of 5CB, induced by the protonation and deprotonation of HA at the interface of aqueous/LC droplet. Because of its high pH sensitivity, the LC-based sensor was further exploited for monitoring the local pH changes, which were originated from concentration changes of acidic gases. The results suggested that at an industrial scale/production environment, the sensor shows excellent performance for long-term monitoring and sensing of acidic gases. This type of LC-based sensor may find to fulfill its applicability potential in the trace measurement of acidic gases.

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“…Liquid crystals (LCs) have been widely applied to various fields, such as flat panel displays [1,2], electro-optical devices [3][4][5], biological [6,7] or environmental sensors [8,9], and topological defects [10,11] in a micrometer scale. Blue phase LCs (BPLCs) are among the most promising materials for advanced applications because of their superior characteristics, such as self-assembled three-dimensional (3D) photonic structure and fast response time [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

All-Optically Controllable Photonic Crystals Based on Chiral-Azobenzene-Doped Blue Phase Liquid Crystals

Jiang

et al. 2020

Crystals

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In this study, the all-optical control properties of photonic crystals based on self-assembled chiral-azobenzene-doped blue phase liquid crystals (CA-BPLCs) were investigated. The difference in the photothermal characteristics of CA-BPLCs with and without homogeneous surface alignment was discussed. Results revealed that surface alignment could induce more uniform and diverse blue phase (BP) structures, including BPII, BPI, and BPS-like phases during cooling. Consequently, the temperature range of BP was wider than that of the sample without surface alignment. All-optical control experiments with light illumination were then performed on the aligned or nonaligned CA-BPLC samples. During continuous irradiation with light beams at wavelengths of 405 and 450 nm, CA dopants underwent trans→cis and cis→trans back photoisomerizations, respectively. These processes promoted isothermal phase transition and wavelength shifting, which further enabled the all-optical control of the CA-BPLC samples. Various optical control modes of BPLC could be achieved through phase change and wavelength shifting by appropriately selecting the working temperature and surface treatment of BPLC. This study could be further used as a basis for developing photoswitchable and tunable BPLC photonic devices, such as light-controllable gratings, filters, mirrors, and lasers.

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Detecting trypsin at liquid crystal/aqueous interface by using surface-immobilized bovine serum albumin

Cited by 36 publications

References 34 publications

State-of-the-Art Development in Liquid Crystal Biochemical Sensors

State-of-the-Art Development in Liquid Crystal Biochemical Sensors

Liquid-crystal-droplet-based Monitoring System for Water-soluble Inorganic Acidic Gases from the Atmosphere

All-Optically Controllable Photonic Crystals Based on Chiral-Azobenzene-Doped Blue Phase Liquid Crystals

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