Detection and analysis of mycovirus-related RNA viruses from grape powdery mildew fungus Erysiphe necator

Pandey, Binod; Naidu, Rayapati A.; Grove, Gary G.

doi:10.1007/s00705-018-3714-0

Cited by 15 publications

(10 citation statements)

References 51 publications

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“…The host of PcCV1, Penicillium HS-CQ15, was isolated from Penicillium -decayed citrus surface (Chongqing, China) and molecularly characterized as P. crustosum species, according to internal transcribed spacer (ITS) analysis described before (Gardes and Bruns, 1993; Pandey et al, 2018). HS-CQ15 conidial suspension stored in glycerol at −70°C was initially cultured on potato dextrose agar (PDA) medium at 28°C and 180 rpm for about 7 days, and the resulting mycelium fragments were transferred into potato dextrose broth (PDB) medium for additional 96 h cultivation at the same conditions.…”

Section: Methodsmentioning

confidence: 99%

Molecular Characterization of a Chrysovirus Isolated From the Citrus Pathogen Penicillium crustosum and Related Fungicide Resistance Analysis

Wang

Yang

Zhang

et al. 2019

Front. Cell. Infect. Microbiol.

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Penicillium sp. are damaging to a range of foods and fruits including citrus. To date, double-stranded (ds)RNA viruses have been reported in most Penicillium species but not in citrus pathogen P. crustosum . Here we report a novel dsRNA virus, designated as Penicillium crustosum chrysovirus 1 (PcCV1) and isolated from P. crustosum strain HS-CQ15. PcCV1 genome comprises four dsRNA segments, referred to as dsRNA1, dsRNA2, dsRNA3, and dsRNA4, which are 3600, 3177, 3078, and 2808 bp in length, respectively. Sequence analysis revealed the presence of four open reading frames (ORFs) in the PcCV1 genome. ORF1 in dsRNA1 encodes a putative RNA-dependent RNA polymerase (RdRp) and ORF2 in dsRNA2 encodes a putative coat protein (CP). The two remaining ORFs, ORF3 in dsRNA3 and ORF4 in dsRNA4, encode proteins of unknown function. Phylogenetic analysis based on RdRp sequences showed that PcCV1 clusters with other members of the genus Chrysovirus , family Chrysoviridae . Transmission electron microscope (TEM) analysis revealed that the PcCV1 visions are approximately 40 nm in diameter. Regarding biological effects of PcCV1, HS-CQ15 harboring the chrysovirus exhibited no obvious difference in colony morphology under fungicide-free conditions but decreased resistance to demethylation inhibitor (DMI)-fungicide prochloraz, as compared to PcCV1-cured strain. Here we provide the first evidence of a virus present in citrus pathogenic fungus P. crustosum and the chrysovirus-induced change in fungicide-resistance of its host fungus.

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Section: Methodsmentioning

confidence: 99%

Molecular Characterization of a Chrysovirus Isolated From the Citrus Pathogen Penicillium crustosum and Related Fungicide Resistance Analysis

Wang

Yang

Zhang

et al. 2019

Front. Cell. Infect. Microbiol.

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“…Network inference (Section 3.3) suggested an antagonistic interaction between powdery mildew and the yeast Buckleyzyma aurantiaca (Pauvert et al, 2020), which remains to be experimentally validated with culturedependant approaches (Section 3.4). Moreover, as for Plasmopara viticola, the virome of E. necator has been studied in search of hypovirulence-inducing mycoviruses (Pandey et al, 2018). Mycoviruses have been detected but their influence on fungal virulence and disease development has not been demonstrated yet.…”

Section: Seasonal Assembly Of the Leaf Microbiome In Interaction With...mentioning

confidence: 99%

The functional microbiome of grapevine throughout plant evolutionary history and lifetime

Fournier

Pellan

Barroso-Bergadà

et al. 2022

Advances in Ecological Research

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“…In recent years, mycoviruses have drawn increased attention for the effects they can have on their host virulence [9]; some can significantly reduce virulence (e.g., the dsRNA virus DK21 of Fusarium graminearum [10]), while others can significantly increase it (e.g., the dsRNA mycovirus RsRV2 found in the rice sheath blight isolate D122 strain [11]). These findings have spurred the development of hypovirulence-associated mycoviruses for use as biocontrol agents [12–14].…”

Section: Introductionmentioning

confidence: 99%

A novel double-stranded RNA mycovirus isolated from Trichoderma harzianum

Liu

Redda

et al. 2019

Virol J

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Background Trichoderma spp. are used extensively in agriculture as biological control agents to prevent soil-borne plant diseases. In recent years, mycoviruses from fungi have attracted increasing attention due to their effects on their hosts, but Trichoderma mycoviruses have not been the subject of extensive study. We sought to discover novel mycoviruses from Trichoderma spp. and to determine the effects of the biocontrol function of Trichoderma spp. Methods Mycoviruses were screened by dsRNA extraction and metagenomic analysis. RT-PCR, 5′ RACE, and 3′ RACE were used to obtain the genome sequence. MEGA software was used to classify the new mycovirus. The effects of the identified mycovirus on the biological properties of the host strain 525 were evaluated using cucumber plants and Fusarium oxysporum f. sp. cucumerinum. Results A novel mycovirus, Trichoderma harzianum mycovirus 1 (ThMV1) (accession number MH155602), was discovered in Trichoderma harzianum strain 525, a soil-borne fungus collected from Inner Mongolia, China. The mycovirus exhibited a double-stranded RNA (dsRNA) genome with a complete genome sequence of 3160 base pairs and two open reading frames (ORFs) on the negative strand. Phylogenetic analysis indicated that it belongs to an unclassified family of dsRNA mycoviruses. The removal of ThMV1 from the host 525 strain reduced host biomass production and improved the biocontrol capability of the host for Fusarium oxysporum f. sp. cucumerinum. At same time, the presence of ThMV1 improved the growth of cucumber. Conclusion ThMV1 is a new unclassified mycovirus found in T. harzianum. It not only affects the phenotype of the host strain but also reduces its biocontrol function, which sheds light on the interaction between the mycovirus and Trichoderma spp.

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Detection and analysis of mycovirus-related RNA viruses from grape powdery mildew fungus Erysiphe necator

Cited by 15 publications

References 51 publications

Molecular Characterization of a Chrysovirus Isolated From the Citrus Pathogen Penicillium crustosum and Related Fungicide Resistance Analysis

Molecular Characterization of a Chrysovirus Isolated From the Citrus Pathogen Penicillium crustosum and Related Fungicide Resistance Analysis

The functional microbiome of grapevine throughout plant evolutionary history and lifetime

A novel double-stranded RNA mycovirus isolated from Trichoderma harzianum

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