Detection and Analysis of the Bacterium,<i>Xylella fastidiosa</i>, in Glassy-Winged Sharpshooter,<i>Homalodisca vitripennis</i>, Populations in Texas

Hail, Daymon; Mitchell, Forrest L.; Lauzière, Isabelle; Marshall, Patrick; Brady, Jeff A.; Bextine, Blake

doi:10.1673/031.010.14128

Cited by 6 publications

(3 citation statements)

References 26 publications

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“…Publications were filtered by selecting only records where the pathogen detection on symptomatic grapevines was confirmed by PCR or Elisa. The exact coordinates of the records were taken when available in the publication or approximated to locality or county level to build the Supplementary Data 1 19,39,41,[75][76][77][78][79] . For modelling purposes and to attempt a general rough estimate of the R 0 parameter valid for the entire US, we assumed a single vector with a uniform spatial distribution.…”

Section: Methodsmentioning

confidence: 99%

Global predictions for the risk of establishment of Pierce’s disease of grapevines

Giménez-Romero

Galván

Montesinos³

et al. 2022

Commun Biol

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The vector-borne bacterium Xylella fastidiosa is responsible for Pierce’s disease (PD), a lethal grapevine disease that originated in the Americas. The international plant trade is expanding the geographic range of this pathogen, posing a new threat to viticulture worldwide. To assess the potential incidence of PD, we have built a dynamic epidemiological model based on the response of 36 grapevine varieties to the pathogen in inoculation assays and on the vectors’ distribution when this information is available. Key temperature-driven epidemiological processes, such as PD symptom development and recovery, are mechanistically modelled. Integrating into the model high-resolution spatiotemporal climatic data from 1981 onward and different infectivity (R0) scenarios, we show how the main wine-producing areas thrive mostly in non-risk, transient, or epidemic-risk zones with potentially low growth rates in PD incidence. Epidemic-risk zones with moderate to high growth rates are currently marginal outside the US. However, a global expansion of epidemic-risk zones coupled with small increments in the disease growth rate is projected for 2050. Our study globally downscales the risk of PD establishment while highlighting the importance of considering climate variability, vector distribution, and an invasive criterion as factors to obtain better PD risk maps.

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Section: Methodsmentioning

confidence: 99%

Global predictions for the risk of establishment of Pierce’s disease of grapevines

Giménez-Romero

Galván

Montesinos³

et al. 2022

Commun Biol

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“…Model calibration and validation. To attempt a rough estimate of the R 0 parameter in the United States, assuming a uniform spatial distribution of the vectors, we ran several model simulations validating the spatiotemporal distribution of PD from data collected from publications between 2001 and 2015 [19,39,41,[61][62][63][64][65]. We found R 0 = 8 as the optimal parameter for maximising the area under a ROC curve (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Global predictions for the risk of establishment of Pierce’s disease of grapevines

Giménez-Romero

Galván

Montesinos³

et al. 2022

Preprint

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The vector-borne bacterium Xylella fastidiosa is responsible for Pierce’s disease (PD), a lethal grapevine illness that originated in the Americas. The international plant trade is expanding the geographic range of this pathogen, posing a new threat to viticulture worldwide. To assess the potential incidence of PD, we have built a dynamic epidemiological model based on the response of 36-grapevine varieties to the pathogen in inoculation assays and on the vectors’ distribution when this information is available. Key temperature-driven epidemiological processes, such as PD symptom development and recovery, are mechanistically modelled. Integrating into the model highresolution spatiotemporal climatic data from 1981 onward and different infectivity (R0) scenarios, we show how the main wine-producing areas thrive mostly in non-risk, transient, or epidemic-risk zones with potentially low growth rates in PD incidence. Epidemic-risk zones with moderate to high growth rates are currently marginal outside the United States. However, a global expansion of epidemic-risk zones coupled with small increments in the disease growth rate is projected for 2050. Our study globally downscales the risk of PD establishment while highlighting the importance of considering climate variability, vector distribution and an invasive criterion in obtaining accurate risk maps to guide policy decision-making in plant health.

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“…A 1-l aliquot was used from each sample in 50-l PCR reactions. Primers used for pyrosequencing were Gray28F 5Ј TTT GAT CNT GGC TCA G 3Ј and Gray519R 5Ј GTN TTA CNG CGG CKG CTG 3Ј (Dowd et al 2008;Hail et al 2010Hail et al , 2011. Initial generation of the sequencing library used a one-step PCR with a total of 30 cycles using HotStar Taq Plus Master Mix (Qiagen) and the following thermal protocol: 94ЊC for 3 min followed by 30 cycles of 94ЊC for 30 s; 60ЊC for 40 s and 72ЊC for 1 min; and a Þnal elongation step at 72ЊC for 5 min.…”

Section: Methodsmentioning

confidence: 99%

A Global Comparison ofBactericera cockerelli(Hemiptera: Triozidae) Microbial Communities

Arp

Munyaneza²,

Crosslin³

et al. 2014

Environ Entomol

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The potato psyllid (Bactericera cockerelli Sulc) is an economically important insect pest of solanaceous crops such as potato, tomato, pepper, and tobacco. Historically, the potato psyllid's range included central United States, Mexico, and California; more recently, populations of this insect have been reported in Central America, the Pacific Northwest, and New Zealand. Like most phytophagous insects, potato psyllids require symbiotic bacteria to compensate for nutritional deficiencies in their diet. Potato psyllids harbor the primary symbiont, Candidatus Carsonella ruddii, and may also harbor many secondary symbionts such as Wolbachia sp., Sodalis sp., Pseudomonas sp., and others. These secondary symbionts can have an effect on reproduction, nutrition, immune response, and resistances to heat or pesticides. To identify regional differences in potato psyllid bacterial symbionts, 454 pyrosequencing was performed using generic 16S rRNA gene primers. Analysis was performed using the Qiime 1.6.0 software suite, ARB Silva, and R. Operational taxonomic units were then grouped at 97% identity. Representative sequences were classified to genus using the ARB SILVA database. Potato psyllids collected in California contained a less diverse microbial community than those collected in the central United States and Central America. The crop variety, collection year, and haplotype did not seem to affect the microbial community in potato psyllids. The primary difference between psyllids in different regions was the presence and overall bacterial community composition of Candidatus Carsonella ruddii and Wolbachia.

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Detection and Analysis of the Bacterium,Xylella fastidiosa, in Glassy-Winged Sharpshooter,Homalodisca vitripennis, Populations in Texas

Cited by 6 publications

References 26 publications

Global predictions for the risk of establishment of Pierce’s disease of grapevines

Global predictions for the risk of establishment of Pierce’s disease of grapevines

Global predictions for the risk of establishment of Pierce’s disease of grapevines

A Global Comparison ofBactericera cockerelli(Hemiptera: Triozidae) Microbial Communities

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