Detection and antibiotic susceptibility of coliform bacteria in fresh vegetables

Falomir, María Pilar; Gozalbo, Daniel; Sebastiá, Carlos; Rico, Hortensia

doi:10.1142/9789814322119_0080

Cited by 6 publications

(7 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…"Listeria species has been associated with a wide variety of food sources particularly poultry, red meat, and meat products. In Egypt, Listeria contamination rate in meat and chicken products was reported to be 41%, lower than the 73.9% reported in Malaysia from imported frozen beef and 83.3% from raw minced meat in Turkey" [2,3]. "The bacteria can be endemic in food processing environments, because it survives food-processing technologies that rely on acidic or salty conditions and, unlike many pathogens, can continue to multiply slowly at low temperatures, allowing for growth even in properly refrigerated foods.…”

Section: Introductionmentioning

confidence: 82%

Phenotypic and Genotypic Virulence Potentials of Listeria monocytogenes Isolated from Different Food Samples in Yola

Isa,

Bashir,

Kachalla

2024

SAJRM

View full text Add to dashboard Cite

Aim: the aim of this work is to determine the Phenotypic and Genotypic Virulence Potentials of Listeria monocytogenes Isolated from Different Food Samples in Yola. Study Design: the study was a randomized study; the samples (sources of organisms) were collected at random from different market and shops within the study area. Place and Duration of Study: the study was carried out in Yola, the capital of Adamawa State, north eastern Nigeria. Methodology: phenotypic virulence potentials of the isolates was determined by haemolytic activity on 5% sheep blood agar and biofilm formation ability, using Christensen’s tube method and microtiter plate method for the qualitative and quantitative biofilm formation respectively. Genotypic virulence determination was done by polymerase chain reaction amplification of plcB, hlyA and actA genes. Results: All the isolates (100%) were beta haemolytic on 5% sheep blood agar and all the isolates were biofilm formers but with different degree of biofilm formation abilities. Genotypically all the isolates (100%) indicated the presence of all the three virulence genes amplified. Conclusion: food samples isolates analyzed are potentially virulence pathogenic of L. monocytogenes that can pose food borne listeriosis to high risk individuals in the study area.

show abstract

Section: Introductionmentioning

confidence: 82%

Phenotypic and Genotypic Virulence Potentials of Listeria monocytogenes Isolated from Different Food Samples in Yola

Isa,

Bashir,

Kachalla

2024

SAJRM

View full text Add to dashboard Cite

show abstract

“…E. cloacae is ubiquitous in terrestrial and aquatic environments and occurs as a commensal in the intestines of humans and animals, making it a perfect candidate for transfer from irrigated produce to humans. E. cloacae have been isolated from ready-to-eat salads served in a primary school in Valencia city [ 23 ] and from vegetables irrigated with untreated wastewater in Morocco [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Coupled DNA-labeling and sequencing approach enables the detection of viable-but-non-culturable Vibrio spp. in irrigation water sources in the Chesapeake Bay watershed

Malayil

Chattopadhyay

Mongodin

et al. 2021

Environmental Microbiome

View full text Add to dashboard Cite

Nontraditional irrigation water sources (e.g., recycled water, brackish water) may harbor human pathogens, including Vibrio spp., that could be present in a viable-but-nonculturable (VBNC) state, stymieing current culture-based detection methods. To overcome this challenge, we coupled 5-bromo-2′-deoxyuridine (BrdU) labeling, enrichment techniques, and 16S rRNA sequencing to identify metabolically-active Vibrio spp. in nontraditional irrigation water (recycled water, pond water, non-tidal freshwater, and tidal brackish water). Our coupled BrdU-labeling and sequencing approach revealed the presence of metabolically-active Vibrio spp. at all sampling sites. Whereas, the culture-based method only detected vibrios at three of the four sites. We observed the presence of V. cholerae, V. vulnificus, and V. parahaemolyticus using both methods, while V. aesturianus and V. shilonii were detected only through our labeling/sequencing approach. Multiple other pathogens of concern to human health were also identified through our labeling/sequencing approach including P. shigelloides, B. cereus and E. cloacae. Most importantly, 16S rRNA sequencing of BrdU-labeled samples resulted in Vibrio spp. detection even when our culture-based methods resulted in negative detection. This suggests that our novel approach can effectively detect metabolically-active Vibrio spp. that may have been present in a VBNC state, refining our understanding of the prevalence of vibrios in nontraditional irrigation waters.

show abstract

“…Fresh vegetables usually harbor natural non-pathogenic epiphytic microorganisms, but during growth, harvest, transport and further handling the produce can be contaminated with pathogens from animal and human sources [ 17 ]. Raw produces can be contaminated with Vibrio cholerae at any points of production chain, from farm to consumer’s mouth, with Vibrio cholerae being viable for 2–5 days on the contaminated produce [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Cholera outbreak in Addis Ababa, Ethiopia: A case-control study

2020

View full text Add to dashboard Cite

Background Cholera remains a significant public health problem in more than one-third of the countries of the world. Cholera outbreak has become more common in Addis Ababa particularly in the rainy seasons; however, there is a paucity of data on risk factors associated with cholera outbreaks rendering interventions difficult. We investigated the outbreak to identify its etiology, source, risk factors and in order to control the outbreak. Methods We compared cases with health center-based unmatched controls (1:2). Cases were patients aged �5 years with acute watery diarrhea, with or without vomiting while controls were persons aged �5 years without history of acute watery diarrhea. We interviewed our study participants using structured questionnaire to collect demographic and cholera risk factors data. We described the outbreak over time, and then tested our hypotheses using unconditional logistic regression.

show abstract

Detection and antibiotic susceptibility of coliform bacteria in fresh vegetables

Cited by 6 publications

References 19 publications

Phenotypic and Genotypic Virulence Potentials of Listeria monocytogenes Isolated from Different Food Samples in Yola

Phenotypic and Genotypic Virulence Potentials of Listeria monocytogenes Isolated from Different Food Samples in Yola

Coupled DNA-labeling and sequencing approach enables the detection of viable-but-non-culturable Vibrio spp. in irrigation water sources in the Chesapeake Bay watershed

Cholera outbreak in Addis Ababa, Ethiopia: A case-control study

Contact Info

Product

Resources

About