Detection and Assessment of Interleukin 6 in Irreversible Pulp Inflamation

Abstract

The study aimed to assess the number, localization and distribution of interleukin 6 (IL-6) positive cells in healthy pulp, acute and chronic pulpitis. The study group included 48 patients aged between 18-72, treated in University of Medicine and Pharmacy Grigore T. Popa Iasi, Romania. The pulpectomy was performed on 42 patients diagnosed with acute and chronic pulpitis. The other 6 patients, without signs of dental caries or periodontal disease, were submitted to extractions of teeth for orthodontic purposes, with pulpectomy performed before extraction. The pulp samples were examined with optic microscope. The detection and assessment of IL-6 were performed using immunohistochemical technique. Data were statistically analysed using non-parametric tests. According to morphopathological criteria, 42.85% were classified as acute pulpitis and 57.14% as chronic pulpitis. The pulp samples in control group were not associated with IL-6 positive cells. The analysis of all samples with acute and chronic pulpitis identified 73.80% samples with IL-6 and 26.20% associated with the absence of IL-6. The highest frequency of IL-6 positive cells was recorded in rich-cell zone of crown dental pulp. The systemic distribution of IL-6 positive cells was mostly diffused without well-defined orientation. IL-6 release in acute and chronic pulpitis is significantly higher comparing with healthy pulp tissue.

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Anti‐inflammatory effects of the prostacyclin analogue iloprost in an in vitro model of inflamed human dental pulp cells

“…Both gram-negative and gram-positive bacteria in a caries lesion produce pathogens that activate the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) signalling pathway, resulting in the production of pro-inflammatory cytokines, such as IL-6 and IL-12 [15][16][17][18]. The expression of IL-6 is higher in acute pulpitis compared with chronic pulpitis and healthy human dental pulp tissue [19]. Streptococcus mutans is one of the major pathogens for dental caries and upregulates the expression of IFNγ, which induces IL-12 expression [20].…”

Effect of bioactive glasses used as dentin desensitizers on the dentin-pulp complex in rats

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