Detection and assessment of the antibiotic resistance of Enterobacteriaceae recovered from bioaerosols in the Choqueyapu River area, La Paz – Bolivia

Medina, Claudia; Ginn, Olivia; Brown, Joe; Soria, Freddy; Garvizu, Carolina; Salazar, Ángela Skantria; Tancara, Alejandra; Herrera, Jhoana

doi:10.1016/j.scitotenv.2020.143340

Cited by 16 publications

(8 citation statements)

References 26 publications

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“…Our previous studies in La Paz and Kanpur reported the detection of culturable Enterobacteriaceae and E. coli in ambient urban air that was hypothesized to also originate from OWCs. − A study in Malawi measured the presence of enteric microbes in ambient air (enterotoxigenic E. coli) before, during, and after pit latrine emptying, confirming that these microbes increased in nearby aerosols during pit latrine emptying events .…”

Section: Discussionmentioning

confidence: 85%

Detection and Quantification of Enteric Pathogens in Aerosols Near Open Wastewater Canals in Cities with Poor Sanitation

Ginn

Rocha-Melogno

Bivins

et al. 2021

Environ. Sci. Technol.

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Urban sanitation infrastructure is inadequate in many low-income countries, leading to the presence of highly concentrated, uncontained fecal waste streams in densely populated areas. Combined with mechanisms of aerosolization, airborne transport of enteric microbes and their genetic material is possible in such settings but remains poorly characterized. We detected and quantified enteric pathogen-associated gene targets in aerosol samples near open wastewater canals (OWCs) or impacted (receiving sewage or wastewater) surface waters and control sites in La Paz, Bolivia; Kanpur, India; and Atlanta, USA, via multiplex reverse-transcription qPCR (37 targets) and ddPCR (13 targets). We detected a wide range of enteric targets, some not previously reported in extramural urban aerosols, with more frequent detections of all enteric targets at higher densities in La Paz and Kanpur near OWCs. We report density estimates ranging up to 4.7 × 102 gc per mair 3 across all targets including heat-stable enterotoxigenic Escherichia coli, Campylobacter jejuni, enteroinvasive E. coli/Shigella spp., Salmonella spp., norovirus, and Cryptosporidium spp. Estimated 25, 76, and 0% of samples containing positive pathogen detects were accompanied by culturable E. coli in La Paz, Kanpur, and Atlanta, respectively, suggesting potential for viability of enteric microbes at the point of sampling. Airborne transmission of enteric pathogens merits further investigation in cities with poor sanitation.

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Section: Discussionmentioning

confidence: 85%

Detection and Quantification of Enteric Pathogens in Aerosols Near Open Wastewater Canals in Cities with Poor Sanitation

Ginn

Rocha-Melogno

Bivins

et al. 2021

Environ. Sci. Technol.

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show abstract

“…A small number of previous studies have identified the likely presence of aerosolized fecal material and potential for pathogen transmission in bioaerosols in similar settings. Our previous studies in La Paz and Kanpur reported detection of culturable Enterobacteriaceae and E. coli in ambient urban air that was hypothesized to also originate from OWCs 113–115 . A study in Malawi measured the presence of enteric microbes in ambient air (enterotoxigenic E. coli ) before, during, and after pit latrine emptying, confirming that these microbes increased in nearby aerosols during pit latrine emptying events 58 .…”

Section: Discussionmentioning

confidence: 99%

Detection and quantification of enteric pathogens in aerosols near open wastewater canals in cities with poor sanitation

Ginn

Rocha-Melogno

Bivins

et al. 2021

Preprint

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Urban sanitation infrastructure is inadequate in many low-income countries, leading to the presence of highly concentrated, uncontained fecal waste streams in densely populated areas. Combined with mechanisms of aerosolization, airborne transport of enteric microbes and their genetic material is possible in such settings but remains poorly characterized. We detected and quantified enteric pathogen-associated gene targets in aerosol samples near open wastewater canals (OWCs) or impacted surface waters and control sites in La Paz, Bolivia; Kanpur, India; and Atlanta, USA via multiplex qPCR (37 targets) and ddPCR (13 targets). We detected a wide range enteric pathogen-specific targets, some not previously reported in extramural urban aerosols, with more frequent detections of all enteric targets at higher densities in La Paz and Kanpur near OWCs. We report density estimates ranging from non-detects to 4.7 x 102 gc per m3air for targets including ST-ETEC, C. jejuni, EIEC/Shigella spp., Salmonella spp., norovirus, and Cryptosporidium spp. An estimated 25%, 76%, and 0% of samples containing positive pathogen detects were accompanied by culturable E. coli in La Paz, Kanpur, and Atlanta, respectively, suggesting potential for viability of enteric microbes at the point of sampling. Airborne transmission of enteric pathogens merits further investigation in cities with poor sanitation.

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“…Although high-volume sample collection and methodologically sophisticated pathogen assays may often be unavailable in such settings, affordable and accessible detection of culturable fecal indicator bacteria and phenotypic resistance testing can provide valuable insight into potential health impacts for exposed populations. 58,134,135 Studies included in this review reporting on enteric microbes including pathogens in bioaerosols in urban settings targeted a limited range of enteric microbes, with many studies (38%) reporting detection of commonly used fecal indicator bacteria (Escherichia coli and Enteroccocus). Only one study in a tropical urban setting identified, through sequencing, the pathogenic strain of Escherichia coli, O157:H7 (EHEC) yielding a limited data pool that excludes other strains such as ETEC, EPEC and others that were responsible for over 60,000 deaths across the globe in 2016.…”

Section: Discussionmentioning

confidence: 99%

A systematic review of enteric pathogens and antibiotic resistance genes in outdoor urban aerosols

Ginn¹,

Lowry²,

Brown³

2022

Environmental Research

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Detection and assessment of the antibiotic resistance of Enterobacteriaceae recovered from bioaerosols in the Choqueyapu River area, La Paz – Bolivia

Cited by 16 publications

References 26 publications

Detection and Quantification of Enteric Pathogens in Aerosols Near Open Wastewater Canals in Cities with Poor Sanitation

Detection and Quantification of Enteric Pathogens in Aerosols Near Open Wastewater Canals in Cities with Poor Sanitation

Detection and quantification of enteric pathogens in aerosols near open wastewater canals in cities with poor sanitation

A systematic review of enteric pathogens and antibiotic resistance genes in outdoor urban aerosols

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