Detection and characterisation of CTX-M and CMY-2 β-lactamases among Escherichia coli isolates from farm animals in Guangdong Province of China

Liu, Jianhua; Wei, Shuyong; Ma, Junying; Zeng, Zhenling; Lü, Di; Yang, Guang; Zhang-liu, Chen

doi:10.1016/j.ijantimicag.2006.12.015

Cited by 114 publications

(97 citation statements)

References 15 publications

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“…The diverse CTX-M enzymes detected in our study confirmed that the globally prevalent CTX-M-14 (22,42) and CTX-M-15 (32,37) enzymes are quite common in China, as reported previously (46). CTX-M-22, CTX-M-27, and CTX-M-65 have been reported on relatively few occasions (46), but mainly from China, and all have previously been observed among food animals in China (23,25,39). Thus, the detection of these enzymes in our isolates may indicate that food animals are an important reservoir for cephalosporin-resistant E. coli in China.…”

Section: Discussionsupporting

confidence: 90%

Molecular Characterization and Antimicrobial Susceptibility Testing of Escherichia coli Isolates from Patients with Urinary Tract Infections in 20 Chinese Hospitals

Cao

Cavaco

et al. 2011

J Clin Microbiol

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A total of 222 urinary Escherichia coli isolates from 20 tertiary hospitals in 15 different provinces and 4 municipalities in mainland China were characterized by antimicrobial susceptibility, phylogrouping, and the presence of plasmid-mediated quinolone resistance genes. A subset of 138 suspected extended-spectrum cephalosporinase (ESC) producers were examined for genes encoding cephalosporin resistance. Forty-three isolates harboring bla CTX-M-14 or bla CTX-M-15 were analyzed by pulsed-field gel electrophoresis (PFGE), and plasmids containing these genes were typed using PCR-based replicon typing (PBRT). Thirteen phylogroup B2 bla CTX-M-14 -and bla CTX-M-15 -positive isolates were analyzed by multilocus sequence typing (MLST). A frequent occurrence of resistance (>46%) was observed toward cephalosporins, gentamicin, and fluoroquinolones. Among the 222 isolates, 4 qnrS1, 4 qepA, and 16 aac(6)-Ib-cr genes were confirmed. Four major phylogroups (A, B1, B2, and D) and nontypeable isolates (NTs) were found among the isolates, with phylogroup D (54%) being the most common phylogroup. A total of 110 (80%) of the 138 screened isolates harbored bla CTX-M genes, with bla CTX-M-14 (71%) and bla CTX-M-15 (24%) being the most prevalent of these genes. Nine of the 13 CTX-M-15-or CTX-M-14-containing B2 isolates belonged to ST131. PFGE typing showed a high level of diversity, and plasmid analysis indicated a very large pool of different resistance plasmids mediating the spread of bla CTX-M genes in mainland China. An equally very high frequency of resistance and equally high levels of diversity in phylogroups, PFGE types, and plasmids were observed among community-and hospitalacquired E. coli isolates, indicating the presence of a large reservoir in the community and a long-term spread of cephalosporin resistance in China.

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Section: Discussionsupporting

confidence: 90%

Molecular Characterization and Antimicrobial Susceptibility Testing of Escherichia coli Isolates from Patients with Urinary Tract Infections in 20 Chinese Hospitals

Cao

Cavaco

et al. 2011

J Clin Microbiol

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“…The multiple antibiotic resistances of E. coli demonstrated in the current study is in agreement with other studies from various parts of the world (6,20,21).…”

Section: Discussionsupporting

confidence: 93%

Antibiotic Resistance Pattern of Escherichia coli Groups A, B1, B2 and D Isolated from Frozen Foods and Children with Diarrhea in Sanandaj, Iran

Darehabi¹,

Naseri²,

Menbari³

et al. 2013

Int J Enteric Pathog

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Background: Escherichia coli is usually a commensal of the digestive system, particularly intestine of humans and animals. However, there are many reports indicating that some strains are known to cause serious problems causing various infections among children worldwide, particularly in the developing countries. Escherichia coli strains can be classified into four phylogenetic groups: A, B1, B2 and D. The commensal strains are usually placed into the phylogenetic groups; A and B1 and the extraintestinal pathogenic strains into a group B2 and, to a lesser extent, group D. Objectives: The present study aimed to investigate the antibiotic resistance pattern of Escherichia coli groups A, B1, B2 and D from frozen foods and children with diarrhea. Materials and methods: From 125 frozen foods of animal origin and 466 rectal swabs from children with diarrhea, E. coli were isolated and identified based on standard procedures. Susceptibility testing to antibiotic was carried out according to the CLSI criteria. The phylogenetic group of each strain was determined by using multiplex PCR. Results: In the current study, 47 and 99 E. coli strains were isolated and allocated into four phylogenetic groups (i.e. A, B1, B2 and D). Among the strains isolated from the frozen foods of animal origin, 6.3% were allocated into phylogenetic group B2, and 23.4%% into D. Similarly, among the strains isolated from children with diarrhea 9% were allocated into group B2 and 19% to group D. The data showed that 55.3% and 53.1% of the frozen food-derived E. coli strains were resistant to Tetracycline and Amoxycillin respectively. Similarly, 89.9, 88.9 and 79.8% of isolates were resistant to tetracycline, chloramphenicol, and ampicillin, respectively. By a multiplex PCR procedure different phylogenetic E. coli were detected. Conclusion: Detection of E. coli isolates is very important and shows that food of animal origin can be of a reservoir for resistant bacteria that potentially could be transferred to humans through the food chain. In addition, results of the current study also revealed the detection of resistant E. coli isolates from children with diarrhea.

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“…The most prevalent resistance genes encoding ESBL in E. coli isolates were bla CTX-M-14 and bla CTX-M-15 , which was consistent with the results of other studies conducted in China, such as the finding of bla CTX-M-14 as the major genotype in genotypic study of bla CTX-M in E. coli originating from animals of Guangdong province, China [21]. Similarly, bla CTX-M-14 was the major bla CTX-M genotype of E. coli isolated from cows with mastitis in China [22].…”

Section: Discussionsupporting

confidence: 90%

Prevalence and antimicrobial resistance profiles of Escherichia coli isolated from free-range pigs

Zhang

Zhao

Wang

et al. 2017

J Infect Dev Ctries

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Introduction: Numerous studies about antimicrobial resistant Escherichia coli (E. coli) of animal origins have been conducted around the world, most of them focus on bacteria from animals raised in intensive breeding farms, but systematic studies on antimicrobial resistance in E. coli of free range animals are still lacking. Methodology: This study aimed to investigate the prevalence and antimicrobial resistance profiles of E. coli from free-range pigs in Laiwu mountainous areas, eastern China. Results: Among 123 fecal samples, 123 non-duplicate E. coli were obtained with an isolation rate of 100.0％ (123/123). These E. coli showed the highest resistance rate to tetracycline (77/123, 62.6％), but all were sensitive to amoxicillin/clavulanic acid. Thirty-eight E. coli (38/123, 30.9％) showed multidrug resistance (MDR). Among 123 E. coli isolates, only 39 carried antimicrobial resistant genes detected in this study. Of these 39 isolates, blaTEM-1, qnrB, qnrD, qnrS1, floR and cfr genes were detected in 13, 9, 4, 7, 10, 7, 20, and 7 isolates, respectively. blaTEM-1 and blaCTX-M-14 genes were concomitantly detected in 6 isolates, and blaTEM, qnrB, qnrS and qnrD genes were concomitantly detected in 7 isolates. Conclusions: Free-ranging pigs may be regarded as a potential reservoir for antibiotic resistant genes.

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Detection and characterisation of CTX-M and CMY-2 β-lactamases among Escherichia coli isolates from farm animals in Guangdong Province of China

Cited by 114 publications

References 15 publications

Molecular Characterization and Antimicrobial Susceptibility Testing of Escherichia coli Isolates from Patients with Urinary Tract Infections in 20 Chinese Hospitals

Molecular Characterization and Antimicrobial Susceptibility Testing of Escherichia coli Isolates from Patients with Urinary Tract Infections in 20 Chinese Hospitals

Antibiotic Resistance Pattern of Escherichia coli Groups A, B1, B2 and D Isolated from Frozen Foods and Children with Diarrhea in Sanandaj, Iran

Prevalence and antimicrobial resistance profiles of Escherichia coli isolated from free-range pigs

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