Detection and characterisation of the biopollutant Xenostrobus securis (Lamarck 1819) Asturian population from DNA Barcoding and eBarcoding

Abstract

DNA efficiently contributes to detect and understand marine invasions. In 2014 the potential biological pollutant pygmy mussel (Xenostrobus securis) was observed for the first time in the Avilés estuary (Asturias, Bay of Biscay). The goal of this study was to assess the stage of invasion, based on demographic and genetic (DNA Barcoding) characteristics, and to develop a molecular tool for surveying the species in environmental DNA. A total of 130 individuals were analysed for the DNA Barcode cytochrome oxidase I gene in order to determine genetic diversity, population structure, expansion trends, and to inferring introduction hits. Reproduction was evidenced by bimodal size distributions of 1597 mussels. High population genetic variation and genetically distinct clades might suggest multiple introductions from several source populations. Finally, species-specific primers were developed within the DNA barcode for PCR amplification from water samples in order to enabling rapid detection of the species in initial expansion stages.

Metabarcoding and post-sampling strategies to discover non-indigenous species: A case study in the estuaries of the central south Bay of Biscay

“…Early detection of invasive species is a crucial step for successful post-introduction management (Pochon, Zaiko, Hopkins, Banks, & Wood, 2015). Genetic analysis has been demonstrated to be a useful tool for inventorying biodiversity (Thorpe, Solé-Cava, & Watts, 2000;Feral, 2002) and for studying marine biological invasions (Dlugosch & Parker, 2008;Estoup & Guillemaud, 2010;Semeraro et al, 2016;Ardura et al, 2016;Devloo-Delva et al, 2016), and the advent of next generation sequencing technologies (NGS) and the possibility of directly analysing DNA from water and sediments (environmental DNA, eDNA) has opened new possibilities for early NIS detection in marine ecosystems, where invasions might remain unnoticed for extended periods (Taberlet, Coissac, Pompanon, Brochmann, & Willerslev, 2012;Freire, Genzano, Neumann-Leitao, & Perez, 2014;Zaiko et al, 2015). Furthermore, detecting short, species-specific eDNA fragments from aquatic environments is theoretically more sensitive than traditional survey methods, which can be both time-consuming and costly (Ardura, Linde, Moreira, & Garcia-Vazquez, 2010;Pochon, Bott, Smith, & Wood, 2013).…”

Barcodes of marine invertebrates from north Iberian ports: Native diversity and resistance to biological invasions

“…Thus early detection of invasive species is a crucial step for successful post-introduction management (e.g. Pochon et al, 2015;Devloo-Delva et al, 2016). For the successful prevention of new invasions it is especially important to identify the main features of recipient communities that may make them resistant to invasions.…”

An Easy Phylogenetically Informative Method to Trace the Globally Invasive Potamopyrgus Mud Snail from River’s eDNA

“…The use of specific primers on eDNA has been successfully demonstrated for a number of species. Examples are fish Petromyzon marinus and Salmo trutta [ 32 ], molluscs such as Rangia cuneata in the Baltic Sea [ 33 ] and Xenostrobus securis in North Spain [ 34 ], and others. Potamopyrgus antipodarum has also been detected previously directly from water samples [ 35 ], as presence-absence based on positive or negative PCR amplification of a fragment of the cytochrome b gene.…”