Detection and Characterization of Antibodies to PEG-IFN-alpha2b Using Surface Plasmon Resonance

Takacs, Melisa A.; Jacobs, Sheila J.; Bordens, Ronald; Swanson, Steven J.

doi:10.1089/107999099313631

Cited by 26 publications

(8 citation statements)

References 16 publications

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“…Recent work has shown that macromolecules such as poly(ethylene glycol) can be used to improve significantly the bioavailability and pharmacokinetics of known drugs 90, 91. A clear example, already a rousing commercial success, is Schering‐Plough's PEG Intron, in which a short strand of monomethoxy poly(ethylene glycol) [PEG; molecular weight = 12,000 Da] is attached to interferon α‐2b, a water‐soluble protein (molecular weight = 19,271 Da) produced by recombinant DNA techniques 92. The polymer–drug conjugate is much more effective that the parent interferon alone in the treatment of hepatitis C.…”

Section: Nanomedicine: Dendrimers In Macromolecular Therapeuticsmentioning

confidence: 99%

Dendrimers and other dendritic macromolecules: From building blocks to functional assemblies in nanoscience and nanotechnology

Fréchet

2003

J. Polym. Sci. A Polym. Chem.

373

280

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Given their size, in the single-digit nanometer range, and the versatility of their functionality, dendrimers and other dendritic macromolecules are poised to make a significant contribution to the rapidly expanding fields of nanoscience and nanotechnology. This highlight focuses on nascent applications of dendrimers that take advantage of their structural features and polyvalent character. In particular, the concept of dendritic encapsulation of function, borrowed from Nature, can be applied to the design of a varied array of energy-harvesting, lightemitting, or catalytic macromolecules. Similarly, the compact size and hierarchical ordering of components within dendrimers make them ideal for exploring the limits of nanolithography. Finally, the presence of differentiated functionalities and the polyvalent character of dendrons and dendrimers constitute strong assets for their use in polymer therapeutics.

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Section: Nanomedicine: Dendrimers In Macromolecular Therapeuticsmentioning

confidence: 99%

Dendrimers and other dendritic macromolecules: From building blocks to functional assemblies in nanoscience and nanotechnology

Fréchet

2003

J. Polym. Sci. A Polym. Chem.

373

280

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“…Previously, SPR was used to know the binding partners and its relative interaction strength for IFNs (Takacs et al, 1999;Asokan et al, 2006;Schmeisser et al, 2007), immune response against IFN-β (Gibbs and Oger, 2008) and in one particular instance to quantify IFN-γ (Stigter et al, 2005). However, reports on the use of SPR in the quantification of IFN-α2b are not available in the literature.…”

Section: Introductionmentioning

confidence: 99%

A simple method for quantification of interferon-2b through surface plasmon resonance technique

Ramanan¹,

Ling²,

Tey³

et al. 2010

Afr. J. Biotechnol.

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A rapid and efficient immunoassay method for quantification of interferon-α2b using surface plasmon resonance was developed with BIAcore 3000 as a sensor. Two different levels of anti-interferon monoclonal antibody were immobilized onto a CM5 chip using an amine coupling method. Similar binding ratio was observed for both the ligand densities. There was no steric hindrance and loss of antibody activity even at higher ligand density (> 22,000 RU). The sensitivity of the assay was increased up to 45% with the increment in ligand density from 15,400 to 22,360 RU. The binding between interferon-α2b and anti-interferon monoclonal antibody was predominantly controlled by mass transfer rate and the relationship was found linear, ranged from 5 to 400 ng/mL. Total cycle time per analysis was less than 8 min and required only 5 µL of sample injection.

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“…PEG has been used extensively because it extends the half-life of most proteins and results in a greatly increased plasma presence (Greenwald, 2001 (Caliceti et al, 1999;Monfardini et al, 1995;Calogerpoulou et al, 2003). The successful application of α-interferon pegylation by ScheringPlough (PEG-INTRON®;molecular weight of PEG 12 kDa daltons;Takacs et al, 1999) and HoffmanLaRoche (Pegasys®;molecular weight of PEG 40 kDa;Bailon PS & Palleroni AV [1997] We (Chong & Chu 2004;Chu et al, 2005) …”

Section: A Nanoparticle Drug-delivery System Is a Device In Which A Dmentioning

confidence: 99%

PAMAM Dendrimers and Branched Polyethyleneglycol (Nanoparticles) Prodrugs of (-)-β-D-(2R, 4R)-dioxolane-thymine (DOT) and Their Anti-HIV Activity

Liang

Janarthanan

Rapp

et al. 2006

Antivir Chem Chemother

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The synthesis, characterization, anti-HIV activity and cytotoxicity of dendrimers of (-)-β β-D-(2R,4R)-dioxolane-thymine (DOT) and polyethylene glycol (PEG)-DOT conjugates are described. Dendrimers in this study were polyamidoamine (PAMAM) generation 2.0, 3.0, 5.0 and 6.0, along with 8.0-branched PEG with a molecular weight of 40 kDa. DOT was attached to PAMAM dendrimers or branched PEG via ester or phosphate groups. Size exclusion chromatography was used to purify the dendrimers and PEG conjugates, which were characterized by NMR and MALDI-TOF mass spectrometry. The synthesized PAMAM dendrimers and PEG conjugates were evaluated for anti-HIV activity against HIV-1 LAI in primary human peripheral blood mononuclear cells (PBMCs) and cytotoxicity in PBMCs, CEM and Vero cells. PAMAM dendrimers of DOT with ester linkages and particularly phosphate linkers showed an increase in anti-HIV potency in comparison with DOT alone (140-and 56-fold, respectively). Unfortunately, the PAMAM dendrimers also exhibited increased cytotoxicity. Anti-HIV activity of PEG-DOT conjugates was found to be lower than that of DOT.

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Detection and Characterization of Antibodies to PEG-IFN-alpha2b Using Surface Plasmon Resonance

Cited by 26 publications

References 16 publications

Dendrimers and other dendritic macromolecules: From building blocks to functional assemblies in nanoscience and nanotechnology

Dendrimers and other dendritic macromolecules: From building blocks to functional assemblies in nanoscience and nanotechnology

A simple method for quantification of interferon-2b through surface plasmon resonance technique

PAMAM Dendrimers and Branched Polyethyleneglycol (Nanoparticles) Prodrugs of (-)-β-D-(2R, 4R)-dioxolane-thymine (DOT) and Their Anti-HIV Activity

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