Detection and Characterization of Cercospora citrullina Isolates That Sporulate Readily in Culture

Goode, M. J.; Brown, G. R.

doi:10.1094/phyto-60-1502

Cited by 10 publications

(3 citation statements)

References 2 publications

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“…In India, CLS was reported 11 to be an issue in majority of mungbean-growing states and even rose to epidemic proportions in Uttar Pradesh from 1934 and 1941. The isolation and detection of C. canescens is difficult due to its slow growth and poor sporulating nature in culture media 12 . The latter can be explained as sporulation of C. canescens is based on isolates rather than influenced by culture conditions 13 .…”

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Draft Genome Sequence of <I>Cercospora canescens</I>: A Leaf Spot Causing Pathogen

Chand¹,

Pal²,

Singh³

et al. 2015

Current Science

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Cercospora canescens (Ellis and Martin ) is a hemibiotrophic pathogen causing leaf spot disease on mungbean (Vigna radiata L). Genome sequence (~33.97 Mb) assembled in 8239 contigs with 10627 protein coding genes. A total of 2842 proteins were identified as homologous of 223 predicted and 7562 putative uncharacterized involved in biological processes, molecular functions. The identified proteins are mainly involved in infection process used to compromise nutrients or destroy host tissues, the gycosidases, transposases and cytochrome P450s genes codes to signal transduction, cell wall breakdown, transporters, host stomata perception, adhesion, polyketide synthase and cercosporin. A total of 528 simple sequence repeats were also identified from the genome sequence assembly of C. canescens. This study provides insights into pathogenic mechanism and a better understanding of virulence differentiation of C. canescens. It will also help in identification of similarity and differences in regions among the genomes of different species of Cercospora.

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confidence: 99%

Draft Genome Sequence of <I>Cercospora canescens</I>: A Leaf Spot Causing Pathogen

Chand¹,

Pal²,

Singh³

et al. 2015

Current Science

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“…Symptoms of the new strain were dry necrotic, grey irregular sporulating spots without chlorotic margins. The pathogen was isolated from infected leaves using the method of Goode & Brown (1970) and purified by single spore isolation in the laboratory.…”

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confidence: 99%

First report of a new pathogenic variant of Cercospora canescens on mungbean (Vigna radiata) from India

Chand

Singh

Pal

et al. 2012

New Disease Reports

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Atypical symptoms were observed on the recently released mungbean (Vigna radiata)cv. Meha that were attributed to the pathogen Cercospora canescens during the sampling of Cercospora leaf spot disease. The disease severity caused by the putative new strain of C. canescens (isolate NFCCI-2370) varied from 60 to 80% at the farm of the Indian Institute of Pulses Research Kanpur, India. Similar atypical symptoms were also observed at neighbouring research stations where seed multiplication of cv. Meha is performed. Yield losses from the new strain varied from 50-70% at these sites that was significantly higher than with the previously identified typical strain. Symptoms of the new strain were dry necrotic, grey irregular sporulating spots without chlorotic margins. The pathogen was isolated from infected leaves using the method of Goode & Brown (1970) and purified by single spore isolation in the laboratory.Isolates of the pathogen were confirmed as C. canescens by productionofcercosporin (Daub, 1982) and typical conidia and conidiophores (Ellis & Martin, 1882). Inoculation on to plants of cvs. Meha, Kopergaon and Malvia Joyoti with a monoconidial culture of the new strain produced the atypical symptoms described (Fig. 1a), whereas inoculation with the typical C. canescens isolates (MTCC-10835) produced several small, angular, necrotic water-soaked grey sporulating lesions surrounded by chlorotic margins (Fig. 1b). A morphological comparison of the new strain (NFCCI-2370) with the typical strain (MTCC-10835) was done using mycelium from infected leaves. The new strain was distinguished from the typical strain by 1-2 scars on the conidiophores, whereas the number of scars was 2-7 in the typical strain. The conidiophores (90.09 µm x 9.64 µm) and conidia (128.94 µm x 5.49 µm) were also shorter in the new strain (Table 1). The new strain bears a single conidium at the tip of conidiophores with a prominent scar (Fig. 2). The radial growth, colony morphology and pigment production of the new strain (NFCCI-2370) on PDA at 25°C (Ferrater et al., 2004) was faster, i.e.3.82 mm/day and colony colour was creamy without production of cercosporin or spores (Fig. 3).The identity of the new strain (NFCCI-2370) together with the other isolate (MTCC-10835) was further confirmed by comparison of ITS (Internal Transcribed Spacer) sequence data with reference isolates. BLASTn analysis of the sequence obtained showed 98-99% homology with reference isolatesof C. canescens. The ITS region of rDNA was amplified with primers ITS1/ ITS4 and sequences of these isolates have been deposited in GenBank (Accession Nos. JF747032 and JF747033). Isolate NFCCI-2370 has been deposited with the National Facility for

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“…1). The isolation and detection of Cercospora species is difficult due to its slow growth and poor sporulating nature on culture media (Goode and Brown 1970). A single spore technique (Choi et al 1999) has been reported as more suitable and successful for isolation of slow growing fungi.…”

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Effect of host extract on growth and sporulation of Cercospora lactucae-sativae

Thomas

Saravanakumar

2019

Australasian Plant Dis. Notes

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Cercospora lactucae-sativae is a slow growing hemibiotrophic fungus that proves difficult to isolate and sporulate on many growth media. The study focussed on the use of a simple isolation procedure for C. lactucae-sativae and examined the effect of host extract on growth and sporulation of C. lactucae-sativae on PDA and Water Agar. The mean colony diameter was significantly greater than that of the control treatment for WA + LE at 1%, 2% and 4% only, and for all of the LE concentrations on PDA. The mean conidial concentration was significantly different from the control treatment for all concentrations on WA at and above 2% LE, while on PDA there were no significant differences between any of the treatment including the control. PCR has confirmed that the leaf spot disease affecting lettuce cultivation in Trinidad is caused by C. lactucae-sativae.

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Detection and Characterization of Cercospora citrullina Isolates That Sporulate Readily in Culture

Cited by 10 publications

References 2 publications

Draft Genome Sequence of <I>Cercospora canescens</I>: A Leaf Spot Causing Pathogen

Draft Genome Sequence of <I>Cercospora canescens</I>: A Leaf Spot Causing Pathogen

First report of a new pathogenic variant of Cercospora canescens on mungbean (Vigna radiata) from India

Effect of host extract on growth and sporulation of Cercospora lactucae-sativae

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