Detection and characterization of chicken anemia virus from commercial broiler breeder chickens

Hailemariam, Zerihun; Omar, Abdul Rahman; Hair-Bejo, M.; Giap, Tan Ching

doi:10.1186/1743-422x-5-128

Cited by 41 publications

(29 citation statements)

References 26 publications

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“…The difference in pathogenicity among viral strains of genetic variation is not clear at this time (Miller and Schat, 2004). Eleven variable amino acid positions of VP1 alignment confirming findings from earlier studies were compared with our 32 strains and the 26P4 strain (Table 2; Renshaw et al, 1996;Chowdhury et al, 2003;Natesan et al, 2006;Hailemariam et al, 2008). Because the Korean CIAV clustered with groups II and IIIa had a similar amino acid pattern of I75, L97, I125, Q139, Q144, V157, and A413, except for the JB BJS 1 strain, classification of these 2 groups was not possible using amino acid substitution.…”

Section: Resultssupporting

confidence: 84%

Molecular characterization of chicken infectious anemia viruses detected from breeder and broiler chickens in South Korea

Kim¹,

Kwon

Bae³

et al. 2010

Poultry Science

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In South Korea, 32 sequences of chicken infectious anemia virus (CIAV) from various flocks of breeder and commercial chickens were genetically characterized for the first time. Phylogenetic analysis of the viral protein 1 gene, including a hypervariable region of the CIAV genome, indicated that Korean CIAV strains were separated into groups II, IIIa, and IIIb. Strains were commonly identified in great-grandparent and grandparent breeder farms as well as commercial chicken farms. In the field, CIAV strains from breeder farms had no clinical effects, but commercial farm strains were associated with depression, growth retardation, and anemia regardless of the group from which the strain originated. In addition, we identified 7 CIAV genomes that were similar to vaccine strains from vaccinated and unvaccinated breeder flocks. These data suggest that further studies on pathogenicity and vaccine efficacy against the different CIAV group are needed, along with continuous CIAV surveillance and genetic analysis at breeder farms.

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Section: Resultssupporting

confidence: 84%

Molecular characterization of chicken infectious anemia viruses detected from breeder and broiler chickens in South Korea

Kim¹,

Kwon

Bae³

et al. 2010

Poultry Science

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“…This low prevalence as compared with the previous 42% seroprevalence [6] and the high nested PCR detection of 87% [7] could have been due to the difference in the type of sample used, areas of study, and the sensitivity of detection methods conducted in each study [13]. After about 3 weeks of age, immunocompetent chickens are resistant to disease, but they can acquire asymptomatic infections and transmit the virus horizontally [3-14].…”

Section: Discussionmentioning

confidence: 98%

Molecular epidemiology of chicken anemia virus in commercial farms in China

Eltahir

Qian

Jin

et al. 2011

Virol J

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BackgroundChicken anemia virus (CAV) is the causative agent of chicken infectious anemia (CIA). A high prevalence of CAV has been reported in China. However, VP1 sequences of Chinese isolates show no clear genotype clustering or correlation with geographic origin. Therefore, the present study aimed to detect and characterize CAV isolates from China based on sequence and phylogenetic analysis of the VP1, VP2 and VP3 genes.ResultsOf 460 spleen samples tested by PCR, 47 (10.22%) were found to be positive for CAV. A total of 25 CAV, approximately full genomes, from different commercial farms were characterized. Phylogenetic analysis of the Chinese CAV sequences together with strains from different countries resulted in four distinct groups (A-D) with significant high bootstrap values. The Chinese viral sequences were located as four different clusters within groups A and D. All the Chinese CAV genomes characterized in this study had glutamine (Q) at amino acid position 394, which indicated that all are highly pathogenic. Mutations associated with attenuation and weaker reactivity with monoclonal antibody 2A9 were absent in the Chinese sequences.ConclusionsWe revealed that CAV prevalence was lower than that reported previously in commercial farms in China. We also showed four distinct sequence groups (A-D), and genetic variability in local CAV sequences that could be divided into four groups based on phylogenetic analysis.

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“…Another major amino acid profile (V75, M97, K139 and E144) was also found (Hailemariam et al, 2008;van Santen et al, 2001). However, there is a lack of information related to the antigenicity of CAV strains belonging to these genetic groups.…”

Section: Cav Is a Non-enveloped Virus And Is Classified In The Genusmentioning

confidence: 99%

Characterization of mAbs to chicken anemia virus and epitope mapping on its viral protein, VP1

Trinh

Ogawa

Bui

et al. 2015

Journal of General Virology

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Three (MoCAV/F2, MoCAV/F8 and MoCAV/F11) of four mouse mAbs established against the A2/76 strain of chicken anemia virus (CAV) showed neutralization activity. Immunoprecipitation showed a band at~50 kDa in A2/76-infected cell lysates by neutralizing mAbs, corresponding to the 50 kDa capsid protein (VP1) of CAV, and the mAbs reacted with recombinant VP1 proteins expressed in Cos7 cells. MoCAV/F2 and MoCAV/F8 neutralized the 14 CAV strains tested, whereas MoCAV/F11 did not neutralize five of the strains, indicating distinct antigenic variation amongst the strains. In blocking immunofluorescence tests with the A2/76-infected cells, binding of MoCAV/F11 was not inhibited by the other mAbs. MoCAV/F2 inhibited the binding of MoCAV/ F8 to the antigens and vice versa, suggesting that the two mAbs recognized the same epitope. However, mutations were found in different parts of VP1 of the escape mutants of each mAb: EsCAV/F2 (deletion of T89+A90), EsCAV/F8 (I261T) and EsCAV/F11 (E144G). Thus, the epitopes recognized by MoCAV/F2 and MoCAV/F8 seemed to be topographically close in the VP1 structure, suggesting that VP1 has at least two different neutralizing epitopes. However, MoCAV/F8 did not react with EsCAV/F2 or EsCAV/F8, suggesting that binding of MoCAV/F8 to the epitope requires coexistence of the epitope recognized by MoCAV/F2. In addition, MoCAV/ F2, with a titre of 1 : 12 800 to the parent strain, neutralized EsCAV/F2 and EsCAV/F8 with low titres of 32 and 152, respectively. The similarity of the reactivity of MoCAV/F2 and MoCAV/F8 to VP1 may also suggest the existence of a single epitope recognized by these mAbs.

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Detection and characterization of chicken anemia virus from commercial broiler breeder chickens

Cited by 41 publications

References 26 publications

Molecular characterization of chicken infectious anemia viruses detected from breeder and broiler chickens in South Korea

Molecular characterization of chicken infectious anemia viruses detected from breeder and broiler chickens in South Korea

Molecular epidemiology of chicken anemia virus in commercial farms in China

Characterization of mAbs to chicken anemia virus and epitope mapping on its viral protein, VP1

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