Detection and Characterization of Human Group C Rotaviruses in Bangladesh

Rahman, Mustafizur; Banik, Sukalyani; Faruque, A. S. G.; Taniguchi, Koki; Sack, David A.; Ranst, Marc Van; Azim, Tasnim

doi:10.1128/jcm.43.9.4460-4465.2005

Cited by 74 publications

(81 citation statements)

References 38 publications

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“…However, molecular analysis of human GCRV strains would not seem to support this theory, as all of the human GCRVs form a closely related group, suggesting a strong host-species restriction (2,34). These inconsistencies may be a result of the limited amount of sequence data available for animal strains.…”

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confidence: 81%

“…Human infection by GCRV has been associated with both sporadic episodes and large outbreaks of gastroenteritis in all age groups and appears to be globally distributed (1,2,5,6,18,24,25,34,38,41,43). Large-scale epidemiological studies have revealed that the prevalence may range from 0.6% to 6.8% (2,24), and GCRVs are regarded as emerging human pathogens.…”

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confidence: 99%

“…The porcine GCRVs exhibit a variety of VP7 genotypes (G1, G3, G5, and G6), while the bovine strains identified to date are G2 and the human strains are G4 (22,28,29,33,34,47,48).…”

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confidence: 99%

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Detection and Characterization of Group C Rotaviruses in Asymptomatic Piglets in Ireland

2008

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Group C rotaviruses are important human enteric pathogens that have also been detected in a variety of mammalian species, including pigs. Group C rotaviruses have been identified in piglets with diarrhea, but their ecology remains to be elucidated. By screening of 292 fecal samples collected from 4-to 5-week-old asymptomatic pigs from four herds in Ireland between 2005 and 2007, 13 (4.4%) samples tested positive by reverse transcription-PCR for group C rotavirus. Group A rotaviruses were also detected in 19 samples but not in conjunction with group C viruses. The gene encoding the major group C neutralization antigen, the outer capsid protein VP7, was sequenced. The majority of the strains were very closely related to each other (>99% amino acid [aa] identity) and were characterized as genogroup G1 since they were genetically related to the prototype porcine strain Cowden (92.6% aa identity). Conversely, two strains (1GA/05/Cork/Ire and 281/07/ Dublin/Ire) were characterized as genogroup G6 since they displayed the highest identity (89.2 to 94.0% aa) to porcine G6 strains (43/06-22-like). Unexpectedly, one such G6 strain, 1GA/05/Cork/Ire, lacked the 4-aa insertion in the VP7 variable region VR8 found in all the other G6 group C rotaviruses. This study provides evidence that porcine group C rotavirus may be detected not infrequently in asymptomatic piglets. In addition, it provides evidence that, unlike the human viruses, porcine group C rotaviruses display broad genetic heterogeneity, which may pose a challenge for the development of prophylactic tools.

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confidence: 81%

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confidence: 99%

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Detection and Characterization of Group C Rotaviruses in Asymptomatic Piglets in Ireland

2008

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“…A number of studies on RVC from all over the world by several researchers prove that this group is as important as RVA and causes severe acute gastroenteritis in human and swine (Rahman et al 2005;Meleg et al 2008;Moon et al 2011;Marthaler et al 2013;Suzuki et al 2014). In a routine electrophoretic screening survey for RVA in swine population, we found an atypical migration pattern of genomic RNA segments from a piglet fecal specimen (Figure 1), resembling established electrophoretic pattern (4-3-2-2) of RVC (Desselberger 2014).…”

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confidence: 99%

Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene

Kattoor

Saurabh

Malik

et al. 2017

Veterinary Quarterly

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Background: Rotavirus C (RVC), a known etiological agent of diarrheal outbreaks, mainly inflicts swine population globally with sporadic incidence in human, cattle, ferret, mink and dog. Objective: To demonstrate the presence of RVC in Indian swine population and characterization of its selected structural (VP6) and non-structural (NSP4 and NSP5) genes. Methods: A total of 108 diarrheic samples from different regions of India were used. Isolated RNA was loaded onto polyacrylamide gel to screen for the presence of RVs through the identification of specific electrophoretic genomic migration pattern. To characterize the RVC strains, VP6 gene and NSP4 and NSP5 genes were amplified, sequenced and analyzed. Results: Based on VP6 gene specific diagnostic RT-PCR, the presence of RVC was confirmed in 12.0% (13/108) piglet fecal specimens. The nucleotide sequence analysis of VP6 gene, encoding inner capsid protein, from selected porcine RVC (PoRVC) strains revealed more than 93% homologies to human RVC strains (HuRVC) of Eurasian origin. These strains were distant from hitherto reported PoRVCs and clustered with HuRVCs, owning I2 genotype. However, the two non-structural genes, i.e. NSP4 and NSP5, of these strains were found to be of swine type, signifying a re-assortment event that has occurred in the Indian swine population. Conclusion:The findings indicate the presence of human-like RVC in Indian pigs and division of RVC clade with I2 genotype into further sub-clades. To the best of our knowledge, this appears to be the first report of RVC in Indian swine population. Incidence of human-like RVC VP6 gene in swine supports its subsequent zoonotic prospective.

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“…Group C rotaviruses were first detected from an Australian infant hospitalized with diarrhea in 1973 (33). Since then, group C rotaviruses have been recognized as a cause of gastroenteritis in all age groups, accounting for either sporadic cases or large outbreaks of gastroenteritis in closed and semiclosed communities (8,15,20,22,31,32,(34)(35)(36)38). Cohort and seroepidemiological studies have indicated that virtually all children are infected with group A rotaviruses by 5 years of age (28,39), while the rate of infection with group C rotaviruses is much lower, and antibody prevalence usually peaks at an age of 40 to 50 years or later (14,21,37).…”

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confidence: 99%

Detection and Quantification of Group C Rotaviruses in Communal Sewage

Meleg

Bányai

Martella

et al. 2008

Appl Environ Microbiol

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7 ; median, 6.9 ؋ 10 5 genome equivalents per liter influent sewage) and appeared to display seasonal activity over the study period, whereas animal strains appeared to circulate throughout the year at much lower average titers (bovine strains mean, 9.9 ؋ 10 4 ; median, 3.0 ؋ 10 4 ; porcine strains mean, 3.9 ؋ 10 4 ; median, 3.1 ؋ 10 4 genome equivalents per liter influent sewage). Our findings suggest that monitoring of communal sewage may provide a good surrogate for investigating the epidemiology and ecology of group C rotaviruses in humans and animals.

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Detection and Characterization of Human Group C Rotaviruses in Bangladesh

Cited by 74 publications

References 38 publications

Detection and Characterization of Group C Rotaviruses in Asymptomatic Piglets in Ireland

Detection and Characterization of Group C Rotaviruses in Asymptomatic Piglets in Ireland

Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene

Detection and Quantification of Group C Rotaviruses in Communal Sewage

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