Detection and characterization of hybrid uropathogenic Escherichia coli strains among E. coli isolates causing community-acquired urinary tract infection

Yousefipour, Mahta; Rezatofighi, Seyedeh Elham; Ardakani, Mohammad Roayaei

doi:10.1099/jmm.0.001660

Cited by 13 publications

(9 citation statements)

References 40 publications

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“…Some studies found that the incidence of UTIs was higher in women than in men and was positively associated with higher urine acidity, higher urine calcium levels, lower urinary pH, and longer duration of urolithiasis [20]. In this study, E.coli was the most predominant bacteria with 309 isolates followed by [21]. There are many different strains of bacteria that can cause a UTI, but the most common are Escherichia coli and Klebsiella pneumoniae.…”

Section: Discussionmentioning

confidence: 52%

An Investigation of Bacterial Infections in the Urinary Tract of Babylon City Women in Iraq, a Cross-Sectional Study

Ali

Aljanaby

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Urine infection, also known as a urinary tract infection (UTI), is an inflammation of the urinary system, while it is most common in women, UTI can occur in both men and women. A total of 719 urine samples were collected from women with UTI admitted to a general hospital in Babylon City, Iraq, between March 2022 and February 2023. Standard laboratory methods were used to culture urine and to diagnose all bacterial isolates that were grown on laboratory media. The Kirby-Bauer disc diffusion method was used to test antibiotic susceptibility. Out of a total of 719 urine samples, 646 urine samples with positive bacterial growth and 73 urine samples without bacterial growth. E.coli was the most predominant bacteria with 309 isolates, K.pneumoniae 112, S.saprophyticus 62, E.faecalis 48, P. aeruginosa 31, S.aureus 20, S.haemolyticus 19, St.agalatiae 16 and C. freundii 8 isolates. E.coli, K.pneumoniae and P. aeruginosa were highly resistance to amoxicillin 25 μg with percentages 81%, 82% and 90% respectively; S.saprophyticus had good resistance to penicillin 6 μg and ampicillin 10 μg with percentages 74%. The most effective antibiotic was imipenem 10μg, which inhibited all bacterial growth.

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Section: Discussionmentioning

confidence: 52%

An Investigation of Bacterial Infections in the Urinary Tract of Babylon City Women in Iraq, a Cross-Sectional Study

Ali

Aljanaby

2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…Bacterial adherence assays were performed as previously described with some modifications [49] , [50] . HeLa cells [50,000 cells/well] were infected with the treated and untreated bacteria at 37 °C for 3 h in a 5% CO 2 atmosphere.…”

Section: Methodsmentioning

confidence: 99%

Genomic characterization of a multidrug-resistant uropathogenic Escherichia coli and evaluation of Echeveria plant extracts as antibacterials

Castañeda-Meléndrez,

Magaña-Lizárraga,

Martínez-Valenzuela

et al. 2024

AIMSMICRO

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<abstract> Uropathogenic <italic>Escherichia coli</italic> (UPEC) is the most common bacterial agent associated with urinary tract infections, threatening public health systems with elevated medical costs and high morbidity rates. The successful establishment of the infection is associated with virulence factors encoded in its genome, in addition to antibacterial resistance genes, which could limit the treatment and resolution of the infection. In this sense, plant extracts from the genus <italic>Echeveria</italic> have traditionally been used to treat diverse infectious diseases. However, little is known about the effects of these extracts on bacteria and their potential mechanisms of action. This study aims to sequence a multidrug-resistant UPEC isolate (UTI-U7) and assess the multilocus sequence typing (MLST), virulence factors, antimicrobial resistance profile, genes, serotype, and plasmid content. Antimicrobial susceptibility profiling was performed using the Kirby-Bauer disk diffusion. The antibacterial and anti-adherent effects of the methanol extracts (ME) of <italic>Echeveria</italic> (<italic>E. craigiana</italic>, <italic>E. kimnachii</italic>, and <italic>E. subrigida</italic>) against UTI-U7 were determined. The isolate was characterized as an O25:H4-B2-ST2279-CH40 subclone and had resistant determinants to aminoglycosides, β-lactams, fluoroquinolones/quinolones, amphenicols, and tetracyclines, which matched with the antimicrobial resistance profile. The virulence genes identified encode adherence factors, iron uptake, protectins/serum resistance, and toxins. Identified plasmids belonged to the IncF group (IncFIA, IncFIB, and IncFII), alongside several prophage-like elements. After an extensive genome analysis that confirmed the pathogenic status of UTI-U7 isolate, <italic>Echeveria</italic> extracts were tested to determine their antibacterial effects; as an extract, <italic>E. subrigida</italic> (MIC, 5 mg/mL) displayed the best inhibitory effect. However, the adherence between UTI-U7 and HeLa cells was unaffected by the ME of the <italic>E. subrigida</italic> extract. </abstract>

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“…These hybrids may have the ability to cause disease at multiple sites of infection. Some studies have looked to determine the frequency by which hybrid UPEC are isolated from UTIs, with reported figures between 2% and 10.5% [ 40 , 41 , 44 ].…”

Section: Introductionmentioning

confidence: 99%

Uropathogenic Escherichia coli (UPEC)-Associated Urinary Tract Infections: The Molecular Basis for Challenges to Effective Treatment

Whelan,

Lucey,

Finn

2023

Microorganisms

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Urinary tract infections (UTIs) are among the most common bacterial infections, especially among women and older adults, leading to a significant global healthcare cost burden. Uropathogenic Escherichia coli (UPEC) are the most common cause and accounts for the majority of community-acquired UTIs. Infection by UPEC can cause discomfort, polyuria, and fever. More serious clinical consequences can result in urosepsis, kidney damage, and death. UPEC is a highly adaptive pathogen which presents significant treatment challenges rooted in a complex interplay of molecular factors that allow UPEC to evade host defences, persist within the urinary tract, and resist antibiotic therapy. This review discusses these factors, which include the key genes responsible for adhesion, toxin production, and iron acquisition. Additionally, it addresses antibiotic resistance mechanisms, including chromosomal gene mutations, antibiotic deactivating enzymes, drug efflux, and the role of mobile genetic elements in their dissemination. Furthermore, we provide a forward-looking analysis of emerging alternative therapies, such as phage therapy, nano-formulations, and interventions based on nanomaterials, as well as vaccines and strategies for immunomodulation. This review underscores the continued need for research into the molecular basis of pathogenesis and antimicrobial resistance in the treatment of UPEC, as well as the need for clinically guided treatment of UTIs, particularly in light of the rapid spread of multidrug resistance.

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Detection and characterization of hybrid uropathogenic Escherichia coli strains among E. coli isolates causing community-acquired urinary tract infection

Cited by 13 publications

References 40 publications

An Investigation of Bacterial Infections in the Urinary Tract of Babylon City Women in Iraq, a Cross-Sectional Study

An Investigation of Bacterial Infections in the Urinary Tract of Babylon City Women in Iraq, a Cross-Sectional Study

Genomic characterization of a multidrug-resistant uropathogenic <i>Escherichia coli</i> and evaluation of <i>Echeveria</i> plant extracts as antibacterials

Uropathogenic Escherichia coli (UPEC)-Associated Urinary Tract Infections: The Molecular Basis for Challenges to Effective Treatment

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