Detection and characterization of<i>Kodamaea ohmeri</i>associated with small hive beetle<i>Aethina tumida</i>infesting honey bee hives

Benda, Nicole; Boucias, Drion G.; Torto, Baldwyn; Teal, Peter E. A.

doi:10.1080/00218839.2008.11101459

Cited by 39 publications

(18 citation statements)

References 13 publications

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“…Fermentation processes are generally associated with bacteria, fungi or yeasts. Benda et al (2008) isolated and characterized the yeast Kodamaea ohmeri (NRRL Y-30722) from infested hives and from larval and adult SHB. K. ohmeri is apparently vectored by SHB and grows proliWcally on honeybee combs infested with SHB (Torto et al 2007;Benda et al 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Benda et al (2008) isolated and characterized the yeast Kodamaea ohmeri (NRRL Y-30722) from infested hives and from larval and adult SHB. K. ohmeri is apparently vectored by SHB and grows proliWcally on honeybee combs infested with SHB (Torto et al 2007;Benda et al 2008). If the yeast grows on pollen sources, it produces volatiles, which attract adult SHB (Torto et al 2007).…”

Section: Introductionmentioning

confidence: 99%

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Effects of organic acid treatments on small hive beetles, Aethina tumida, and the associated yeast Kodamaea ohmeri

Schäfer

Ritter

Pettis³

et al. 2009

J Pest Sci

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In honeybee, Apis mellifera, colonies infested with larval and adult small hive beetles (SHB), hive material, and in particular honey, tends to ferment, probably due to SHB-associated yeasts such as the predominant Kodamaea ohmeri. Here, we test the eVects of organic acids on K. ohmeri and on SHB-infested honey/pollen combs. Organic acids were applied at standard concentrations used by beekeepers to control other pests. In laboratory tests, the growth of K. ohmeri was signiWcantly inhibited by lactic, formic and acetic acids. Treatments of SHB-infested honey/ pollen combs (N = 18 colonies) with acetic acid signiWcantly increased mortality of adult SHB and treatments with formic acid signiWcantly reduced larval infestation. Our data suggest that treatment of honeybee colonies and storage rooms with organic acids could also help in reducing SHB damage.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of organic acid treatments on small hive beetles, Aethina tumida, and the associated yeast Kodamaea ohmeri

Schäfer

Ritter

Pettis³

et al. 2009

J Pest Sci

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“…It is often suggested that such associations are mutualistic, with the fungus presumably providing nutritional supplements and the insect vector providing a habitat for the fungus (Vega and Dowd 2005). Yeast symbionts have been found in association with a broad range of insects including lacewings, wasps, bees and beetles Hunter 2005, 2009;Rosa et al 2003;Torto et al 2007;Benda et al 2008). Invertebrates rely on microbes for various metabolic functions, including synthesis of amino acids, vitamins, lipids, sterols and pheromones, degradation of nutritional substrates, and detoxification of compounds (Suh et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Different mosquito species host Wickerhamomyces anomalus (Pichia anomala): perspectives on vector-borne diseases symbiotic control

Ricci

Mosca

Valzano

et al. 2010

Antonie van Leeuwenhoek

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The genetic manipulation of the microbial community associated with hematophagus insects is particularly relevant for public health applications. Within mosquito populations, this relationship has been overlooked until recently. New advances in molecular biotechnology propose the genetic manipulation of mosquito symbionts to prevent the transmission of pathogens to humans by interfering with the obligatory life cycle stages within the insect through the use of effector molecules. This approach, defined as 'paratransgenesis', has opened the way for the investigation and characterization of microbes residing in the mosquito body, particularly those localised within the gut. Some interesting bacteria have been identified as candidates for genetic modification, however, endosymbiotic yeasts remain largely unexplored with little information on the symbiotic relationships to date. Here we review the recent report of symbiotic relationship between Wickerhamomyces anomalus (Pichia anomala) and several mosquito vector species as promising methods to implement control of mosquito-borne diseases.

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“…It is unknown whether a small hive beetle or a honeybee was responsible for the introduction of this yeast into the beehive. Because yeasts are commonly found in flowers and their associated insects, and because either can become contaminated during the common behavioral interaction between honeybees and small hive beetles, either or both routes of entry are feasible (Benda et al 2008;Lachance et al 2001b).…”

Section: Resultsmentioning

confidence: 99%

“…The bar indicates the sequence dissimilarity value of 0.01 substitutions per site. Saccharomyces cerevisiae CBS 1171 T was used as an outgroup association with the small hive beetle Aethina tumida, a pest of the European honeybee A. mellifera (Benda et al 2008;Torto et al 2007). It is unknown whether a small hive beetle or a honeybee was responsible for the introduction of this yeast into the beehive.…”

Section: Resultsmentioning

confidence: 99%

A novel ascosporogenous yeast species, Zygosaccharomyces siamensis, and the sugar tolerant yeasts associated with raw honey collected in Thailand

et al. 2011

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Diversity of yeasts in association with bees and their food sources has been explored during the last decade. In Thailand, there has been no study of yeast identification in honey and bees. Hence, a total of 186 yeast strains were isolated from 37 honey samples of 12 different bee species. On the basis of morphological and physiological characteristics, 55 representative strains were chosen and identified by sequence analysis of the 26S rDNA D1/D2 domain and the ITS region. The data were compared with the published sequences and the results showed the occurrence of 19 ascomycetous and 1 basidiomycetous yeast species. Six strains of the new species were isolated. Phylogenetic analysis of the 26S rDNA D1/D2 sequence revealed that they were conspecific and most closely related to Zygosaccharomyces mellis. Based on the ITS sequence, the new species was clustered with the type " and clearly distinguished from the type !. Sequence analysis of combined ITS-26S rDNA D1/D2 showed similar results. The occurrence of these two types, with a divergence of more than 1% in their sequences, and low DNA relatedness among them suggested that members of the type β can be regarded as separate species. An analysis of the morphological and physiological characteristics was performed. Ascospore formation was observed on acetate agar and Gorodkowa agar. The new Zygosaccharomyces species differed physiologically from Z. mellis in 4 assimilation tests. This data supports the hypothesis that the new species, Zygosaccharomyces siamensis, is a novel ascosporogenous yeast. The type strain is JCM 16825 T (=CBS 12273 T ) and a description is given here.

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Detection and characterization ofKodamaea ohmeriassociated with small hive beetleAethina tumidainfesting honey bee hives

Cited by 39 publications

References 13 publications

Effects of organic acid treatments on small hive beetles, Aethina tumida, and the associated yeast Kodamaea ohmeri

Effects of organic acid treatments on small hive beetles, Aethina tumida, and the associated yeast Kodamaea ohmeri

Different mosquito species host Wickerhamomyces anomalus (Pichia anomala): perspectives on vector-borne diseases symbiotic control

A novel ascosporogenous yeast species, Zygosaccharomyces siamensis, and the sugar tolerant yeasts associated with raw honey collected in Thailand

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