Detection and comparison of EGFR mutations in matched tumor tissues, cell blocks, pleural effusions, and sera from patients with NSCLC with malignant pleural effusion, by PNA clamping and direct sequencing

Diagnostic value of circulating free DNA for the detection of EGFR mutation status in NSCLC: a systematic review and meta-analysis

“…As shown in Figure 1, after primary screening, 34 full-text articles10131415162223242526272829303132333435363738394041424344454647484950 were selected for further evaluation of eligibility. By rigorous evaluation, 20 eligible studies were identified and included in meta-analysis1314151622232425262728293031323334353637.…”

“…By rigorous evaluation, 20 eligible studies were identified and included in meta-analysis1314151622232425262728293031323334353637. The main reasons for exclusion were: EGFR mutation status was not detected in tissues1038394041(5), insufficient data to construct 2 × 2 tables42434445(4), duplicate reports464748(3), cfDNA not detected49 (1), and not matched tissues and cfDNA50(1). No additionally studies were identified by searching the references of eligible studies or relevant review.…”

Simultaneous diagnostic platform of genotyping EGFR, KRAS, and ALK in 510 Korean patients with non‐small‐cell lung cancer highlights significantly higher ALK rearrangement rate in advanced stage

“…The amount of DNA used for the study was 200 ng/test (50 ng/exon) for direct sequencing, and 40–80 ng/test (5–10 ng/reaction) for PNA clamping. The extracted DNA was stored at −20°C until it was used, as previously described .…”

Pre-analytical issues in effusion cytology

“…Analyses have been successfully performed on both cells directly isolated from fresh effusion specimens [60] or on material frozen at -80°C [61]. Both cell blocks and archival smears were used for assessment of EGFR mutation status in 2 other studies [62,63], while cell blocks were used exclusively in the report by Yeo et al [64]. Both cell blocks and supernatants from pleural effusion specimens, as well as plasma and tissue specimens, were studied in another report [65].…”