Detection and differentiation of antibodies to human T-cell lymphotropic virus types I and II by the immunofluorescence method

Abstract

We compared the sensitivities of the prototype human T-cell lymphotropic virus type I (HTLV-I)- and HTLV-II-transformed cell lines, MT2 and Mo-T, with that of an HTLV-II-infected cell line, clone 19, established in our laboratory, in the immunofluorescence (IF) test for detection of antibody to HTLV-I and HTLV-II. In addition, IF antibody titers with the three antigens were determined, and the results were compared with HTLV-I and HTLV-II typing by polymerase chain reaction (PCR). The MT2 cell line was more sensitive than the two HTLV-II cell lines for detecting HTLV-I antibody by IF, and clone 19 was more sensitive than Mo-T or MT2 for measuring HTLV-II antibody. In the titration study, the antigen that gave the highest titer correlated completely with the HTLV type determined by PCR, indicating that the relatively simple IF titration method can be used for differentiating HTLV-I and HTLV-II antibody in sera and plasmas.

Resistance to Fas-Mediated Apoptosis of Human T-Cell Lines Expressing Human T-Lymphotropic Virus Type-2 (HTLV-2) Tax Protein

“…HTLV-1-infected MT-2, HTLV-2-infected MoT and C19 (Gallo et al, 1991), and HTLV-free H9, Jurkat, CEM, and Molt T-cell lines were cultured (0.5 ϫ 10 6 cells/ml) in RPMI 1640 supplemented with 2 mM of L-glutamine, penicillin 50 units/ml, streptomycin 50 g/ml, and 10% fetal calf serum. The cell lines were kept at 37°C in 5% CO 2 , and the culture medium was changed twice weekly.…”

In vitro photochemical inactivation of cell‐associated human T‐cell leukemia virus Type I and II in human platelet concentrates and plasma by use of amotosalen

“…Cell‐associated HTLV‐I and ‐II preparation. The 2060 HTLV‐I–infected lymphoid cell line, established from a TSP patient from French Guyana, 59 or C19 HTLV‐II–infected cell line, established from peripheral blood mononuclear cells of an IV drug user from California, 60 were cultured in RPMI 1640 (Cambrex Corp., East Rutherford, NJ; Moore et al., Roswell Park Memorial Institute) supplemented with 10 percent heat‐inactivated fetal calf serum (Cambrex Corp.), 100 U per mL penicillin, 0.1 mg per mL streptomycin (Sigma Chemical Co., St. Louis, MO), and 200 U per mL interleukin‐2 (Sigma Chemical Co.) for the 2060 line. HTLV‐I–infected cells (2060) or HTLV‐II–infected cells (C19) were cultured in flasks, pooled, and counted, to obtain a final concentration of 10 6 cells per mL in each PLT unit.…”

Defective Human T-Cell Lymphotropic Virus Type I (HTLV-I) Provirus in 10 Chilean Seronegative Patients with Tropical Spastic Paraparesis or HTLV-I-Associated Myelopathy

“…Determination of antibodies was accomplished by indirect immunofluorescence assay and Western blotting (WB) (8). DNA was extracted from purified PBMC according to a previously described method (5).…”