Detection and Differentiation of Microfilariae in Canine Blood

Jd, Kelly

doi:10.1111/j.1751-0813.1973.tb14671.x

Cited by 22 publications

(19 citation statements)

References 12 publications

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“…The 100% sensitivity at 25 mff ml 21 , is considerably higher than at 160 mff ml 21 (Brown & Barsanti, 1988), but these authors used only a limited visualization time (60 seconds). Another explanation could be mff motility in the capillary tube lasting approximately 20 minutes subsequent to blood sampling (Kelly, 1973). Therefore, any delay in the process may suppress mff motility, and reduce the sensitivity of the method.…”

Section: Discussionmentioning

confidence: 99%

“…The modified Knott's test (four 20-ml sediment aliquots) (Kelly, 1973), DBS (20 ml of fresh whole blood under a cover slip) and QBC tube method (111 ml of whole blood) (Brown & Barsanti, 1988) were tested against undiluted (n ¼ 70) and serially diluted (n ¼ 10) blood samples. The QBC tube was mounted on a glass slide with the use of adhesive material to permit thorough visualization.…”

Section: Testing For Microfilariaementioning

confidence: 99%

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Comparative methodology for the detection and differentiation of circulating microfilariae of Dirofilaria immitis in the dog

et al. 2004

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The sensitivities of the Knott's test (four 20-microl sediment aliquots), quantitative buffy coat capillary tube method (QBC tube, 111 microl of whole blood) and direct blood smear (DBS, 20 microl of whole blood) were evaluated for the detection of microfilaraemia in dogs. Undiluted whole blood samples taken from 70 Dirofilaria immitis antigen-positive dogs and 10 serially diluted microfilaraemic blood samples at concentrations of 400, 200, 100, 50, 25 and 12 microfilariae (mff) ml(-1) were examined. For filarial speciation, the buffy coat of QBC tubes was mixed with one drop of methylene blue-formalin solution and examined as a direct smear. In 52/70 microfilaraemic blood samples, the number of mff ranged from 12 to 321987 ml(-1) (median: 3199 ml(-1)). The diagnostic sensitivity of the Knott's test, QBC tube method and DBS in undiluted blood samples attained the 100%, 98% and 92.3% levels, respectively. Eighteen dogs tested amicrofilaraemic by all three methods. At concentrations of 400 mff ml(-1), a 100% sensitivity was found by all three methods, while at 200 mff ml(-1) the Knott's test, QBC tube and DBS were 100%, 100% and 90% sensitive, respectively. The relevant figures at 100 mff ml(-1) were 100%, 100% and 80%, at 50 mff ml(-1) 100%, 100% and 50%, at 25 mff ml(-1) 100%, 100% and 10% and at 12 mff ml(-1) 80%, 50% and 10%. At 50 and 25 mff ml(-1), the DBS was less sensitive compared to the other two methods, while at 12 mff ml(-1), only to the Knott's test. A significant correlation was found between the QBC tube method and Knott's test regarding mff speciation. Therefore, the QBC method may be considered a reliable alternative to the Knott's test for both the detection and speciation of mff in the dog.

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Section: Discussionmentioning

confidence: 99%

Section: Testing For Microfilariaementioning

confidence: 99%

Comparative methodology for the detection and differentiation of circulating microfilariae of Dirofilaria immitis in the dog

et al. 2004

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“…Dirofilaria immitis microfilariae were identified morphologically and distinguished from Acanthocheilonema (syn. Dipetalonema ) reconditum , a filarial parasite of the subcutaneous tissues and fascia of canids, according to existing descriptions [30,31]. Dirofilaria repens is not known to occur in Australia [32].…”

Section: Methodsmentioning

confidence: 99%

Zoonotic Helminth Diseases in Dogs and Dingoes Utilising Shared Resources in an Australian Aboriginal Community

et al. 2018

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The impacts of free-roaming canids (domestic and wild) on public health have long been a concern in Australian Indigenous communities. We investigated the prevalence of zoonotic helminth diseases in dogs and sympatric dingoes, and used radio telemetry to measure their spatial overlap, in an Aboriginal community in the Wet Tropics of Australia. Samples collected from dingoes and dogs showed high levels of infection with the zoonotic hookworm, Ancylostoma caninum. Dingoes were also positive for A. ceylanicum infection (11.4%), but dogs were infection free. Whipworm, Trichuris vulpis, infection was far more prevalent in necropsies of domestic dogs (78.6%) than dingoes (3.7%). Dogs were free from Dirofilaria immitis infection, while dingoes recorded 46.2% infection. Eleven dingoes and seven free-roaming domestic dogs were fitted with Global Positioning System collars and tracked over an extended period. Dingo home-ranges almost completely overlapped those of the domestic dogs. However, dingoes and dogs did not utilise the same area at the same time, and dogs may have avoided dingoes. This spatial overlap in resource use presents an opportunity for the indirect spill-over and spill-back of parasites between dogs and dingoes. Tracking and camera traps showed that the community rubbish tip and animal carcasses were areas of concentrated activity for dogs and dingoes.

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“…Προκαλεί τη διροφι λαρίωση, ένα από τα σημαντικότερα παρασιτικά νοσήμα τα του σκύλου, που απαντά σε πολλές περιοχές του κό σμου και στη χώρα μας 12 .…”

Section: εισαγωγηunclassified

“…Το υπερκείμενο υγρό απορριπτόταν και μετά την προσθήκη στο σωλήνα λίγων σταγόνων κυανού του μεθυ-λενίου, το ίζημα εξεταζόταν στο μικροσκόπιο 10 . Η ταυτο ποίηση των μικροφιλαριών στηρίχθηκε στα μορφολογικά χαρακτηριστικά τους 11,12,13 .…”

Section: υλικα και μεθοδοιunclassified

The prevalence of canine dirofilariosis in the region of Attiki

Diakou

2018

J Hellenic Vet Med Soc

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Blood samples of 900 dogs living in the area of Attiki (Greece) were examined. The dogs were over one year old, of different breeds, both sexes and didn't receive heartworm chemoprophylaxis or chemotherapy. All samples were examined by the modified Knott's method for the detection of microfilariae, while a serological examination (ELISA kit PetChek®, IDEXX) for the detection of parasitic antigen was performed to 552 of them. Six out of 900 (0.7%) of the samples were found positive for Dirofìlaria immitis: in 2 of them both microfilariae and parasitic antigen were detected, in 3 only parasitic antigen and in 1 sample only microfilariae were detected. The animals, in which microfilariae D. immitis were detected, were hunting dogs, often taken for hunting out of Attiki region. Moreover, in 4 of the 900 samples (0,4%) microfilariae Dirofìlaria repens were found. According to these results, the prevalence of D. immitis infection of the dog in Attiki is low. Preventive measures such as chemoprophylaxis against dirofilariosis must be taken in order to keep the infection at low level in Attiki.

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Detection and Differentiation of Microfilariae in Canine Blood

Cited by 22 publications

References 12 publications

Comparative methodology for the detection and differentiation of circulating microfilariae of Dirofilaria immitis in the dog

Comparative methodology for the detection and differentiation of circulating microfilariae of Dirofilaria immitis in the dog

Zoonotic Helminth Diseases in Dogs and Dingoes Utilising Shared Resources in an Australian Aboriginal Community

The prevalence of canine dirofilariosis in the region of Attiki

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