DETECTION AND ENUMERATION OF H2S+ BACTERIA: APPLICATION TO SHEWANELLA PUTREFACIENS (CIP)

Malle, Pierre; Vallé, Michel; Demarque, P.; Eb, P.; Tailliez, R.

doi:10.1111/j.1745-4581.1998.tb00190.x

Cited by 4 publications

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results confm our previous findings (Malle et al 1998) with H,S+ flora and in particular Shewanella putrefaciens, which is very sensitive to additional NaC1 in the medium. In fact, Shewanellaputrefaciens does not grow on PCA and the microbial count noted on PCA-H,S 1.5% is probably in part accounted for by H,S+ flora in addition to Shewanella putrefaciens.…”

Section: Fig 2 Modeling Of the Mean Total Bacterial Counts As A Funsupporting

confidence: 92%

Optimization of the Enumeration of Total Aerobic Bacterial Flora in the Flesh of Seafish

Vallé¹,

Eb²,

Tailliez³

et al. 1998

Rapid Methods Automation Mic

Self Cite

View full text Add to dashboard Cite

The total aerobic flora of seafish flesh is weakly halophilic, and requires on average 1.38% NaCl according to statistical studies. Enumeration is optimal on tryptone soya agar or on NaCl supplemented plate count agar (‐H2S), incubated at 20 and 25C, respectively. Plate count agar (‐H2S) was selected because it can also be used for enumeration of hydrogen sulfide‐producing bacteria by degradation of sulfur‐containing proteins, which are abundant in fish The models employed are sigmoidal. The initial bioburden is too great for there to be a lag phase during storage in ice at 0C. The models show that when the total aerobic microflora count exceeds 100,000 cfu/g, whole or filleted fish stored on ice at 0C are unfit for consumption.

show abstract

Section: Fig 2 Modeling Of the Mean Total Bacterial Counts As A Funsupporting

confidence: 92%

Optimization of the Enumeration of Total Aerobic Bacterial Flora in the Flesh of Seafish

Vallé¹,

Eb²,

Tailliez³

et al. 1998

Rapid Methods Automation Mic

Self Cite

View full text Add to dashboard Cite

show abstract

“…Most effort has been devoted to chemical methods of characterizing freshness: assays of TVBN (total volatile basic nitrogen), TMA (trimethylamine),1 nucleotide derivatives using the H,2 K3 and K′4 ratios and assay of fatty acids 5. Other methods include spectroscopic,6 microbiological,7, 8 texture assessment,9 study of electrical properties10 and determination of biogenic amine composition 11. All these methods are informative but do not precisely characterize freshness and are not effective for all species.…”

Section: Introductionmentioning

confidence: 99%

Determination of volatile compounds to characterize fish spoilage using headspace/mass spectrometry and solid‐phase microextraction/gas chromatography/mass spectrometry

et al. 2005

View full text Add to dashboard Cite

Changes in volatile compounds were monitored in whiting (Merlangius merlangus), cod (Gadus morhua) and mackerel (Scomber scombrus) and related to spoilage. Data are presented from headspace/mass spectrometric (HS/MS) analysis and solid-phase microextraction/gas chromatographic/mass spectrometric (SPME/GC/MS) analysis at two time points (day 0 and day 10) during storage at 4 • C. HS/MS revealed 24 ions that could be used as markers of spoilage. SPME/GC/MS identified 86 compounds, 20 of which could perhaps be used to characterize freshness: principally alcohols, ketones, aldehydes and C 2 -C 11 esters. Compounds common to the three species studied appear to be generated by microbial degradation.

show abstract

New Method for Evaluating Bacterial Reduction of Trimethylamine N‐oxide and Its Application to Bacterial Populations in Fish Muscle

Vallé

Eb²,

Tailliez³

et al. 1999

Rapid Methods Automation Mic

View full text Add to dashboard Cite

Bacterial reduction of trimethylamine N‐oxide (TMAO) cannot be reliably determined try qualitative methods, which are unusable because of problems relating to incubation time, the indicator and the equilibrium of the redox potential. This makes it difficult to reproduce results. These problems have been observed with various semi‐agar media used in the evaluation of TMAO reduction. We propose a new and rapid method of quantitative evaluation by means of assay of trimethylamine (TMA) in a new TMA‐free culture medium. This methodology has been used to evaluate the TMAO‐reducing capacity of different endogenous and exogenous bacterial strains found in fish flesh (Aeromonas hydrophila, Alteromonas communis, Escherichia coli, Flavobacterium branchiophilum, Micrococcus sedentarius, Proteus mirabilis, Pseudomonas fluorescens, Pseudomonas nautica, Serratia marcescens, Shewanella putrefaciens, Vibrio parahaemolyticus). Unlike the qualitative methods, our method showed that all tested strains were able to reduce TMAO. Fish spoilage bacteria can form TMA under anaerobic conditions, as shown by tests using bacterial suspensions from fish (Helicolenus dactylopterus, Merlangus merlangus, Clupea harengus). Such tests can be used to assess fish spoilage.

show abstract

DETECTION AND ENUMERATION OF H₂S⁺ BACTERIA: APPLICATION TO SHEWANELLA PUTREFACIENS (CIP)

Cited by 4 publications

References 14 publications

Optimization of the Enumeration of Total Aerobic Bacterial Flora in the Flesh of Seafish

Optimization of the Enumeration of Total Aerobic Bacterial Flora in the Flesh of Seafish

Determination of volatile compounds to characterize fish spoilage using headspace/mass spectrometry and solid‐phase microextraction/gas chromatography/mass spectrometry

New Method for Evaluating Bacterial Reduction of Trimethylamine N‐oxide and Its Application to Bacterial Populations in Fish Muscle

Contact Info

Product

Resources

About