Detection and Evaluation of Matrix Metalloproteinases Involved in Cruciate Ligament Disease in Dogs Using Multiplex Bead Technology

Breshears, Lee A.; Cook, James L.; Stoker, Aaron M.; Fox, D. B.

doi:10.1111/j.1532-950x.2010.00675.x

Cited by 17 publications

(15 citation statements)

References 43 publications

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“…For the xMAP assays, media samples were mixed with antichemokine, anticytokine, or anti‐MMP monoclonal antibody‐charged 5.6 µm polystyrene microspheres. Streptavidin‐phycoerythrin and a biotinylated polyclonal secondary antibody were added following overnight incubation at 4°C with the polystyrene microspheres . The median fluorescence intensity was used to determine the concentration (pg/ml) of each chemokine and cytokine.…”

Section: Methodsmentioning

confidence: 99%

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Transient expression of the diseased phenotype of osteoarthritic chondrocytes in engineered cartilage

Silverstein

Stoker

Ateshian

et al. 2016

Journal Orthopaedic Research

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Due to the degradation of osteoarthritic (OA) cartilage in post-traumatic OA (PTOA), these tissues are challenging to study and manipulate in vitro. In this study, chondrocytes isolated from either PTOA (meniscal-release (MR) model) or normal (contralateral limb) cartilage of canine knee joints were used to form micropellets to assess the maintenance of the OA chondrocyte phenotype in vitro. Media samples from the micropellet cultures were used to measure matrix metalloproteinase (MMP), chemokine, and cytokine concentrations. Significant differences in matrix synthesis were observed as a function of disease with OA chondrocytes generally synthesizing more extracellular matrix with increasing time in culture. No donor dependent differences were detected. Luminex multiplex analysis of pellet culture media showed disease and time-dependent differences in interleukin (IL)-8, keratinocyte chemoattractant (KC)-like protein, MMP-1, MMP-2, and MMP-3, which are differentially expressed in OA. This memory of their diseased phenotype persists for the first 2 weeks of culture. These results demonstrate the potential to use chondrocytes from an animal model of OA to study phenotype alterations during the progression and treatment of OA.

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Section: Methodsmentioning

confidence: 99%

“…Streptavidin-phycoerythrin and a biotinylated polyclonal secondary antibody were added following overnight incubation at 4˚C with the polystyrene microspheres. 31,32 The median fluorescence intensity was used to determine the concentration (pg/ml) of each chemokine and cytokine.…”

Section: Media Chemokine and Cytokine Analysismentioning

confidence: 99%

Transient expression of the diseased phenotype of osteoarthritic chondrocytes in engineered cartilage

Silverstein

Stoker

Ateshian

et al. 2016

Journal Orthopaedic Research

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“…Interestingly, urine biomarkers provided the largest number of differences between dysplastic and non‐dysplastic dogs in this study. This is highly attractive in that urine can easily be obtained in a non‐invasive manner, even by pet owners, such that clinical application is simple, safe, and effective means to obtain diagnosis …”

Section: Discussionmentioning

confidence: 99%

“…This is highly attractive in that urine can easily be obtained in a non-invasive manner, even by pet owners, such that clinical application is simple, safe, and effective means to obtain diagnosis. [22][23][24][25] Research investigating biomarkers for CHD fall into one of two categories-genetic or protein biomarkers. Genetic biomarkers are being studied to determine if DNA can be used to predict CHD in dogs.…”

Section: Discussionmentioning

confidence: 99%

Protein biomarkers in serum and urine for determining presence or absence of hip dysplasia in a canine model

Ahner

Stoker

Bozynski

et al. 2019

Journal Orthopaedic Research

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This study compares serum and urine concentrations of relevant protein biomarkers among adult dogs with or without radiographic canine hip dysplasia (CHD). Adult (≥2 years of age), client‐owned dogs (n = 74) radiographically categorized as having at least “good” hips (n = 49) or having “mild,” “moderate,” or “severe” hip dysplasia (n = 25) by the Orthopedic Foundation for Animals (OFA). Urine and serum samples were obtained from each dog at a single time‐point and processed and analyzed for relevant protein biomarkers. Urinary concentrations of CTX‐II (p < 0.001) and TIMP‐1 (p = 0.002) were significantly lower in dogs with CHD compared to dogs with no CHD. ROC curve analyses were successful in establishing a panel of four biomarkers (urinary CTX‐I and II, serum MMP‐9, and serum PIICP) with high discriminatory capability for the presence or absence of hip dysplasia in adult dogs (AUC = 0.89). Urine and serum biomarkers can distinguish adult dogs with radiographic CHD from those with no CHD with a sensitivity of 0.95 and specificity of 0.77 using ROC analysis with AUC 0.89. Clinical Significance: This finding suggests that this simple, minimally invasive diagnostic technique has potential for discriminating dysplastic dogs from dogs with normal hips, with possible translational application to humans based on similar etiopathogenesis. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 9999:1–5, 2019.

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“…2 Rupture of the CrCL, which most commonly results from degeneration of the CrCL over time, 4 leads to inflammation, pain, and osteoarthritis. 1,2,5,6 It is a leading cause of stifle joint lameness and is one of the most commonly diagnosed orthopedic conditions. 7,8 Prevalence of CrCL deficiency was reported as 2.55% across all breeds, 9 and management of CrCL-deficient dogs in the United States in 2003 was estimated to cost > $1 billion.…”

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confidence: 99%

Canine stifle joint biomechanics associated with tibial plateau leveling osteotomy predicted by use of a computer model

Brown

Bertocci

Marcellin‐Little

2014

ajvr

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Detection and Evaluation of Matrix Metalloproteinases Involved in Cruciate Ligament Disease in Dogs Using Multiplex Bead Technology

Cited by 17 publications

References 43 publications

Transient expression of the diseased phenotype of osteoarthritic chondrocytes in engineered cartilage

Transient expression of the diseased phenotype of osteoarthritic chondrocytes in engineered cartilage

Protein biomarkers in serum and urine for determining presence or absence of hip dysplasia in a canine model

Canine stifle joint biomechanics associated with tibial plateau leveling osteotomy predicted by use of a computer model

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