Detection and Evaluation of Viable but Non-culturable Escherichia coli O157:H7 Induced by Low Temperature with a BCAC-EMA-Rti-LAMP Assay in Chicken Without Enrichment

Hu, Chen; Zhao, Yuan Yang; Shu, Mei; Zhang, Tian Tian; Yan, Bin; Yan, Ge; Wu, Guo Ping

doi:10.1007/s12161-018-1377-9

Cited by 10 publications

(4 citation statements)

References 35 publications

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“…In future studies, SRCA can be combined with propidium monoazide (PMA) or ethidium monoazide (EMA), which can not only avoid false-positive results caused by dead bacteria but also detect the pathogenic microorganism in the viable but nonculturable (VBNC) state. , Then, the inactivation status of bacteria can be described, which will make up for the lack of distinguishing between the dead and living bacteria. SRCA detection also can be combined with computer software to create a microbial model so that quantitative microbial risk assessment can be carried out.…”

Section: Discussionmentioning

confidence: 99%

Development of the Saltatory Rolling Circle Amplification Assay for Rapid and Visual Detection of Alicyclobacillus acidoterrestris in Apple Juice

Yuan

Zhang

et al. 2020

J. Agric. Food Chem.

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A novel nucleic acid isothermal amplification method based on saltatory rolling circle amplification (SRCA) for rapid and visual detection of Alicyclobacillus acidoterrestris in apple juice was established. Fourteen A. acidoterrestris strains and 44 non-A. acidoterrestris strains were used to confirm the specificity. The sensitivity of SRCA was 4.5 × 10 1 CFU/mL by observing the white precipitate with the naked eye, while it was 4.5 × 10 0 CFU/mL by fluorescence visualization. The detection limit of SRCA in artificially inoculated apple juice was 7.1 × 10 1 and 7.1 × 10 0 CFU/mL via visualization of the white precipitate and fluorescence, respectively. Compared with the traditional PCR method, SRCA exhibited at least a 100-fold higher sensitivity and 100-fold lower detection limit. Seventy samples were investigated for A. acidoterrestris contamination, and the results showed 100% sensitivity, 97.01% specificity, and 97.14% accuracy compared with those by the conventional microbiological cultivation method. Overall, this method is a potentially useful tool for visual and rapid detection of A. acidoterrestris.

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Section: Discussionmentioning

confidence: 99%

Development of the Saltatory Rolling Circle Amplification Assay for Rapid and Visual Detection of Alicyclobacillus acidoterrestris in Apple Juice

Yuan

Zhang

et al. 2020

J. Agric. Food Chem.

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“…The reaction can be monitored in real-time with an inexpensive handheld device, leveraging the simple LAMP process for mobile diagnostics and point-of-need testing [ 81 ]. Since LAMP can amplify a target DNA up to 10 9 copies under isothermal conditions within tens of minutes, it has been successfully applied in detecting food borne pathogens such as E. coli in chicken meat [ 82 ], V. parahaemolyticus in seafood samples [ 83 ], or C. botulinum BoNT/A and BoNT/B genes in fish and honey [ 84 ].…”

Section: Portable Amplification Methodsmentioning

confidence: 99%

Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria

Vidić

Vizzini

Manzano

et al. 2019

Sensors

104

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Foodborne pathogenic bacteria present a crucial food safety issue. Conventional diagnostic methods are time-consuming and can be only performed on previously produced food. The advancing field of point-of-need diagnostic devices integrating molecular methods, biosensors, microfluidics, and nanomaterials offers new avenues for swift, low-cost detection of pathogens with high sensitivity and specificity. These analyses and screening of food items can be performed during all phases of production. This review presents major developments achieved in recent years in point-of-need diagnostics in land-based sector and sheds light on current challenges in achieving wider acceptance of portable devices in the food industry. Particular emphasis is placed on methods for testing nucleic acids, protocols for portable nucleic acid extraction and amplification, as well as on the means for low-cost detection and read-out signal amplification.

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“…To date, more than 100 species of microorganisms, most of which are pathogenic (e.g., Campylobacter , Salmonella , Listeria , Vibrio , Yersinia , and E.coli O157:H7) (Zhao, Zhong, Wei, Lin, & Ding, 2017), have been found to enter into the VBNC state in various food products, such as juices (Nicolo et al., 2011), dairy products (Barron, & Forsythe, 2007; Gunasekera, Sørensen, Attfield, Sørensen, & Veal, 2002), fruits and vegetables (Dinu, & Bach, 2011, 2013), poultry (Chen et al., 2019), and seafoods (Cao et al., 2019). During the processing, transport, and storage of food, the pathogens encounter various types of stressors from various procedures, such as cooking (high temperature), refrigeration (low temperature), fermentation and addition of acidic additives (low pH), and addition of salt (osmolarity) (Begley, & Hill, 2015).…”

Section: Introductionmentioning

confidence: 99%

The diagnostic tools for viable but nonculturable pathogens in the food industry: Current status and future prospects

Rui

Liao

Zhao

et al. 2021

Comp Rev Food Sci Food Safe

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Viable but nonculturable (VBNC) microorganisms have been recognized as pathogenic contaminants in foods and environments. The failure of VBNC cells to form the visible colonies hinders the ability to use conventional media for their detection. Efficient and rapid detection of pathogens in the VBNC state is a prerequisite to ensure the food safety and public health. Despite their nonculturability, VBNC cells have distinct characteristics, such as morphology, metabolism, chemical composition, and gene and protein expression, that have been used as the basis for the development of abundant diagnostic tools. This review covers the current status and advances in various approaches for examining microorganisms in the VBNC state, including but not limited to the methodological aspects, advantages, and drawbacks of each technique. Existing methods, such as direct viable count, SYTO/PI dual staining, and propidium monoazide quantitative polymerase chain reaction (PCR), as well as some techniques with potential to be applied in the future, such as digital PCR, enhanced‐surface Raman spectroscopy, and impedance‐based techniques, are summarized in depth. Finally, future prospects for the one‐step detection of VBNC bacteria are proposed and discussed. We believe that this review can provide more optional methods for researchers and promote the development of rapid, accurate detecting methods, and for inspectors, the diagnostic tools can provide data to undertake risk analysis of VBNC cells.

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Detection and Evaluation of Viable but Non-culturable Escherichia coli O157:H7 Induced by Low Temperature with a BCAC-EMA-Rti-LAMP Assay in Chicken Without Enrichment

Cited by 10 publications

References 35 publications

Development of the Saltatory Rolling Circle Amplification Assay for Rapid and Visual Detection of Alicyclobacillus acidoterrestris in Apple Juice

Development of the Saltatory Rolling Circle Amplification Assay for Rapid and Visual Detection of Alicyclobacillus acidoterrestris in Apple Juice

Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria

The diagnostic tools for viable but nonculturable pathogens in the food industry: Current status and future prospects

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