Detection and expression of Bovine papillomavirus in blood of healthy and papillomatosis-affected cattle

Silva, M. A. R.; Albuquerque, B M F De; Pontes, Nayara Elis Cabral; Coutinho, Luciana C.A.; Leitão, M. C. G.; Reis, Marcio C.; Castro, Roberto Soares de; Freitas, Antonio Carlos de

doi:10.4238/2013.february.28.31

Cited by 16 publications

(26 citation statements)

References 26 publications

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“…All these data suggest a viral activity in blood cells (Stocco dos Santos et al , 1998; Araldi et al , 2013). The presence of BPV DNA sequences in peripheral blood mononuclear cells (PBMCs) was also described in the literature in both bovines (Roperto et al , 2008; Silva et al , 2013a) and equines (Brandt et al , 2008, 2011), reinforcing our results. Following studies also showed the presence of BPV transcripts and the L1 capsid protein in PBMCs, demonstrating the productive infection in blood cells (Roperto et al.…”

Section: Breaking Paradigmssupporting

confidence: 90%

“…, 1998). However, that rate can be higher, once virus infection can be asymptomatic (Araldi et al , 2013; Silva et al , 2013a). Furthermore, the absence of epidemiological studies about BPV distribution could underestimate the real percentage of infected animals, representing a notorious difficulty in attempting to develop vaccines, once we do not know the prevalent virus types in each country.…”

Section: Bpv and Bovine Papillomatosis (Bp)mentioning

confidence: 99%

“…Viral latency is a characteristic shared by BPV (Bogaert et al , 2008b; Araldi et al , 2013; Silva et al , 2013a) and HPV (Maran et al , 1995; Astori et al , 1998; Forslund et al , 2004). However, the virus infection induces DNA damages and genomic instability (Stocco dos Santos, et al , 1998; Melo et al , 2011; Araldi et al , 2013; Campos et al , 2013; Melo et al , 2015).…”

Section: Equine Sarcoid: An Example Of Cross-infectionmentioning

confidence: 99%

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Papillomaviruses: a systematic review

et al. 2017

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In the last decades, a group of viruses has received great attention due to its relationship with cancer development and its wide distribution throughout the vertebrates: the papillomaviruses. In this article, we aim to review some of the most relevant reports concerning the use of bovines as an experimental model for studies related to papillomaviruses. Moreover, the obtained data contributes to the development of strategies against the clinical consequences of bovine papillomaviruses (BPV) that have led to drastic hazards to the herds. To overcome the problem, the vaccines that we have been developing involve recombinant DNA technology, aiming at prophylactic and therapeutic procedures. It is important to point out that these strategies can be used as models for innovative procedures against HPV, as this virus is the main causal agent of cervical cancer, the second most fatal cancer in women.

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Section: Breaking Paradigmssupporting

confidence: 90%

Section: Bpv and Bovine Papillomatosis (Bp)mentioning

confidence: 99%

Section: Equine Sarcoid: An Example Of Cross-infectionmentioning

confidence: 99%

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Papillomaviruses: a systematic review

et al. 2017

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“…HPV DNA is present in the blood of cervical and head and neck cancer patients; however, it is detectable also in the blood of healthy individuals (Cao et al., ; Chen et al., ; Jaberipour et al., ). Similarly, DNA and viral gene expression from different BPV types have been detected in the blood of both healthy and tumour‐bearing cattle and equids (van Dyk et al., ; Silva et al., ). However, the biological meaning of the presence of PVs in the blood is still poorly understood.…”

Section: Introductionmentioning

confidence: 97%

Felis catus papillomavirus type-2 but not type-1 is detectable and transcriptionally active in the blood of healthy cats

Altamura

Jebara

Cardeti

et al. 2017

Transbound Emerg Dis

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Papillomaviruses (PVs) are small DNA viruses that induce benign and/or malignant epithelial tumours in different species, including the domestic cat (Felis catus). To date, five F. catus papillomavirus genotypes have been identified (FcaPV-1 to FcaPV-5). FcaPV-1 is associated with skin and oral benign lesions, while FcaPV-2 infection is widely associated with feline squamous cell carcinomas. Several human and animal PVs have been found in body fluids such as peripheral blood; however, the presence of FcaPVs in non-epithelial tissues has not previously been investigated. The aim of this study was to assess the presence and gene expression of FcaPV-1 and FcaPV-2 in the blood of healthy cats. We detected FcaPV-2 DNA in 26 of 103 (25%) blood samples. Importantly, FcaPV-2 L1, E2, E6 and E7 genes were found to be expressed in 3 (25%), 11 (92%), 6 (50%) and 5 (42%) of the samples available for mRNA analysis, respectively. FcaPV-1 was not detected in any of the blood samples analysed here. The data obtained in this work suggest active and eventually productive infection of FcaPV-2 in the blood of healthy cats, implying a possible role in intra-individual spreading as well as in vertical and horizontal transmission.

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“…About 60% of Brazilian cattle herd are infected by BPV [2]. However, this percentage can be greater, since the virus can lead to asymptomatic infections [11,12].…”

Section: Introductionmentioning

confidence: 99%

Bovine papillomavirus productive infection in cell cultures: First evidences

Araldi¹,

Melo²,

Consonni³

et al. 2017

Virol Res Rev

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Bovine papillomavirus (BPV) is the etiological agent of bovine papillomatosis (BP), infectious disease, characterized by the presence of multiples papillomas that can regress spontaneously or progress to malignances. Although recognized as mutagen, BPV action following cancer initiation remains few explored, since studies about cancer progression and metastasis are based on cell cultures. The lack of attention to in vitro models is a reflection of the papillomavirus replication paradigm, which is dependent of epithelium cell differentiation. Since 2008, we have explored the potential of cell lines derived from BPV-infected neoplasms as model to study the oncogenic process. In this study, we described BPV productive infection in cell lines derived from cutaneous papilloma, fibropapilloma and esophageal carcinoma (EC) in which BPV DNA sequences were previously detected by PCR. Considering that the immunodetection of L1 capsid protein is the main evidence of productive infection, we analyzed the expression of this protein by immunofluorescence and flow cytometry. Results showed the immunodetection of L1 protein in cell lines derived from cutaneous papilloma, fibropapilloma and EC, but not in cells derived from BPV-free normal skin. We also observed the presence of spherical and electron-dense particles, with 41.02-61.94 nm diameter in cytoplasmic vesicles of cells in the sixth passage of cutaneous papilloma, fibropapilloma and EC, being compatible with the expected BPV morphology. Cells derived from BPV-free normal skin, in turn, showed membranous particles up to 75.00 nm not compatible with BPV morphology. These results suggest the BPV productive infection in cells lines derived from BPV-infected neoplasm, reinforcing that these cells are useful models to study the viral biology and pathogenesis.Correspondence to: Rita de Cassia Stocco, Genetics Laboratory, Butantan Institute, São Paulo, 05503-900, Brazil, Tel: +55 11 2627-9701; e-mail: rita. stocco@butantan.gov.br Highlights• Bovine papillomavirus (BPV) cause multiples papillomas that can regress or progress to malignances;• BPV action following cancer initiation remains few explored;• Identification of BPV L1 capsid protein and virion-like particles in cytoplasmic vesicles of cell lines derived from BPVinfected cutaneous papilloma, fibropapilloma and esophageal carcinoma;• Cell lines derived from BPV-infected neoplasm can be considered useful model to study the viral biology and pathology.

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Detection and expression of Bovine papillomavirus in blood of healthy and papillomatosis-affected cattle

Cited by 16 publications

References 26 publications

Papillomaviruses: a systematic review

Papillomaviruses: a systematic review

Felis catus papillomavirus type-2 but not type-1 is detectable and transcriptionally active in the blood of healthy cats

Bovine papillomavirus productive infection in cell cultures: First evidences

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