Detection and Genetic Characterization of Metallo-β-Lactamase IMP-1 and VIM-2 in Pseudomonas aeruginosa Strains From Different Hospitals in Kermanshah, Iran

Abstract

Background:Pseudomonas aeruginosais a frequent nosocomial pathogen that causes severe diseases in many settings. Carbapenems, including meropenem and imipenem, are effective antibiotics against this organism. However, the use of carbapenems has been hampered by the emergence of strains resistant to carbapenemsvia different mechanisms such as the production of metallo-β-lactamases (MBLs), which hydrolyze all carbapenems. Several kinds of MBLs have been reported, among them VIM and IMP types being the most clinically significant carbapenemases.Objectives:We aimed to determine the distribution of blaVIM-2 and blaIMP-1 transferable genes encoding MBLs in P. aeruginosa isolated from three academic hospitals in Kermanshah.Patients and Methods:From 22nd June to 22nd September 2012, 225 isolates of P. aeruginosa were collected. These isolates were tested for antibiotic susceptibility with the Kirby-Bauer disk-diffusion method, and the MBLs were assessed using the imipenem-EDTA double-disk synergy test. The isolates were investigated for blaVIM-2 and blaIMP-1 genes using polymerase chain reaction.Results:Among the 225 isolates, 33.7% (76/225) and 18.1% (41/225) were resistant to imipenem and meropenem, respectively. Of the 76 imipenem-resistant P. aeruginosa strains, 45 (59.2%) were positive for MBLs, 34 (75%) strains carried the blaIMP-1 gene, and 1 (2.2%) strain carried the blaVIM-2 gene.Conclusions:Our results showed that there was a high frequency of IMP-1 positive P. aeruginosa in the different wards of the hospitals.

Comparison Between Phenotypic and Genotypic Methods for the Detection of Metallo Beta Lactamases Producing Pseudomonas Aeruginosa

“…The imipenem disk diffusion screening divided 50 study isolates into two groups: 15 isolates (30%) of imipenem resistant and 35 isolates (70%) of imipenem sensitive P. aeruginosa. Similar results have been described by different authors [10,17,8,18,16] While, lower results were reported by [19] and higher results were published by. [20] So, it was noticed that the prevalence of IPM resistance varies greatly between and within countries and even between hospitals within a community and this could be attributed to the differences in antimicrobial usage and infection control practice in different localities.…”

Phenotypic and Molecular Detection of Metallo-Beta-Lactamase Genes Among Imipenem Resistant Pseudomonas aeruginosa and Acinetobacter baumannii Strains Isolated From Patients with Burn Injuries

“…In the current study, from 278 imipenem-resistant P. aeruginosa and 187 imipenem-resistant A. baumannii isolates, 178 and 85 isolates were MBL-producers, respectively. Large outbreaks by MBL-producing P. aeruginosa strains were described in hospitals in Greece, Italy and Korea (27 was not detected in the isolates. Also, in a similar study in India, 47% of A. baumannii isolates carried bla VIM and 0.9% of them harbored bla IMP (29).…”

“…The bla IMP and bla VIM -producing P. aeruginosa strains are reported worldwide (27). The rapid diagnosis of MBL isolates is helpful to select suitable options for antimicrobial therapy and prevent the spread of MBL strains.…”

Understanding the Epidemiology of Multi-Drug Resistant Gram-Negative Bacilli in the Middle East Using a One Health Approach

“…As for non baumannii species, only one study reported the detection of OXA-23 and SPM producing Acinetobacter nosocomialis in patients with blood infections (Pourabbas et al, 2016). In P. aeruginosa , MBLs were the most common carbapenemases including: IMP (IMP-1 and IMP-55) and VIM variants (VIM-1, VIM-2) (Abiri et al, 2015; Lari et al, 2015; Mirbagheri et al, 2015; Moosavian and Rahimzadeh, 2015; Azizi et al, 2016; Saffari et al, 2016; Kazeminezhad et al, 2017; Dogonchi et al, 2018; Pournajaf et al, 2018; Rostami et al, 2018). Only two studies reported the detection of OXA-23 and SPM-1 in Iranian clinical isolates of P. aeruginosa (Ostad Asadolah-Malayeri et al, 2016; Azimi et al, 2018).…”